The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis
Open accessAbstract
During endocytosis, energy is invested to narrow the necks of cargo-containing plasma membrane invaginations to radii at which the opposing segments spontaneously coalesce, thereby leading to the detachment by scission of endocytic uptake carriers. In the clathrin pathway, dynamin uses mechanical energy from GTP hydrolysis to this effect, assisted by the BIN/amphiphysin/Rvs (BAR) domain-containing protein endophilin. Clathrin-independent endocytic events are often less reliant on dynamin, and whether in these cases BAR domain proteins such as endophilin contribute to scission has remained unexplored. Here we show, in human and other mammalian cell lines, that endophilin-A2 (endoA2) specifically and functionally associates with very early uptake structures that are induced by the bacterial Shiga and cholera toxins, which are both clathrin-independent endocytic cargoes. In controlled in vitro systems, endoA2 reshapes membranes before scission. Furthermore, we demonstrate that endoA2, dynamin and actin contribute in parallel to the scission of Shiga-toxin-induced tubules. Our results establish a novel function of endoA2 in clathrin-independent endocytosis. They document that distinct scission factors operate in an additive manner, and predict that specificity within a given uptake process arises from defined combinations of universal modules. Our findings highlight a previously unnoticed link between membrane scaffolding by endoA2 and pulling-force-driven dynamic scission.
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Self-citationEndophilin marks and controls a clathrin-independent endocytic pathway
Boucrot, E., Ferreira, A. P. A., Almeida-Souza, L., Debard, S., Vallis, Y., Howard, G., Bertot, L., Sauvonnet, N., & McMahon, H. T. (2015) · Nature · doi:10.1038/nature14067 · Cited by 542
Self-citationShiga toxins — from cell biology to biomedical applications
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Self-citationGTP-dependent twisting of dynamin implicates constriction and tension in membrane fission
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Mechanisms of clathrin-mediated endocytosis
Kaksonen, M., & Roux, A. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.132 · Cited by 1,698
Endophilin marks and controls a clathrin-independent endocytic pathway
Boucrot, E., Ferreira, A. P. A., Almeida-Souza, L., Debard, S., Vallis, Y., Howard, G., Bertot, L., Sauvonnet, N., & McMahon, H. T. (2015) · Nature · doi:10.1038/nature14067 · Cited by 542
Self-citationMembrane curvature in cell biology: An integration of molecular mechanisms
Jarsch, I. K., Daste, F., & Gallop, J. L. (2016) · Journal of Cell Biology · doi:10.1083/jcb.201604003 · Cited by 328
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Endocytosis in the context-dependent regulation of individual and collective cell properties
Sigismund, S., Lanzetti, L., Scita, G., & Di Fiore, P. P. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-021-00375-5 · Cited by 132
Metadata
- Authors
- Renard, H.-F., Simunovic, M., Lemière, J., Boucrot, E., Garcia-Castillo, M. D., Arumugam, S., Chambon, V., Lamaze, C., Wunder, C., Kenworthy, A. K., Schmidt, A. A., McMahon, H. T., Sykes, C., Bassereau, P., & Johannes, L.
- Venue
- Nature
- Published
- 2014-12-17
- Volume
- 517
- Issue
- 7535
- Pages
- 493-496
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2063116040
- Cited by
- 326
- References
- 44
- FWCI
- 14.37
- Type
- journal article
- Language
- en
- Open access
- green
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- Renard, H.-F., Simunovic, M., Lemière, J., Boucrot, E., Garcia-Castillo, M. D., Arumugam, S., Chambon, V., Lamaze, C., Wunder, C., Kenworthy, A. K., Schmidt, A. A., McMahon, H. T., Sykes, C., Bassereau, P., & Johannes, L. (2015). Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis. Nature. https://doi.org/10.1038/nature14064
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