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Cited by 580

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

Structure of mammalian respiratory complex I

Open access
Zhu, Jiapeng, Vinothkumar, Kutti R., Hirst, Judy·2016·Nature
DOI 10.1038/nature19095
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Abstract

Complex I (NADH:ubiquinone oxidoreductase), one of the largest membrane-bound enzymes in the cell, powers ATP synthesis in mammalian mitochondria by using the reducing potential of NADH to drive protons across the inner mitochondrial membrane. Mammalian complex I (ref. 1) contains 45 subunits, comprising 14 core subunits that house the catalytic machinery (and are conserved from bacteria to humans) and a mammalian-specific cohort of 31 supernumerary subunits. Knowledge of the structures and functions of the supernumerary subunits is fragmentary. Here we describe a 4.2-Å resolution single-particle electron cryomicroscopy structure of complex I from Bos taurus. We have located and modelled all 45 subunits, including the 31 supernumerary subunits, to provide the entire structure of the mammalian complex. Computational sorting of the particles identified different structural classes, related by subtle domain movements, which reveal conformationally dynamic regions and match biochemical descriptions of the ‘active-to-de-active’ enzyme transition that occurs during hypoxia. Our structures therefore provide a foundation for understanding complex I assembly and the effects of mutations that cause clinically relevant complex I dysfunctions, give insights into the structural and functional roles of the supernumerary subunits and reveal new information on the mechanism and regulation of catalysis.

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Metadata

Authors
Zhu, J., Vinothkumar, K. R., & Hirst, J.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2016-08
Volume
536
Issue
7616
Pages
354-358
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature19095
OpenAlex
W2512187837
Cited by
580
References
45
FWCI
31.90
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Structure of mammalian respiratory complex I. https://app.cochat.ai/papers/10.1038/nature19095
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Zhu, J., Vinothkumar, K. R., & Hirst, J. (2016). Structure of mammalian respiratory complex I. Nature. https://doi.org/10.1038/nature19095

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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