The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Human pluripotent stem cells recurrently acquire and expand dominant negative P53 mutations
Open accessAbstract
Human pluripotent stem cells (hPS cells) can self-renew indefinitely, making them an attractive source for regenerative therapies. This expansion potential has been linked with the acquisition of large copy number variants that provide mutated cells with a growth advantage in culture. The nature, extent and functional effects of other acquired genome sequence mutations in cultured hPS cells are not known. Here we sequence the protein-coding genes (exomes) of 140 independent human embryonic stem cell (hES cell) lines, including 26 lines prepared for potential clinical use. We then apply computational strategies for identifying mutations present in a subset of cells in each hES cell line. Although such mosaic mutations were generally rare, we identified five unrelated hES cell lines that carried six mutations in the TP53 gene that encodes the tumour suppressor P53. The TP53 mutations we observed are dominant negative and are the mutations most commonly seen in human cancers. We found that the TP53 mutant allelic fraction increased with passage number under standard culture conditions, suggesting that the P53 mutations confer selective advantage. We then mined published RNA sequencing data from 117 hPS cell lines, and observed another nine TP53 mutations, all resulting in coding changes in the DNA-binding domain of P53. In three lines, the allelic fraction exceeded 50%, suggesting additional selective advantage resulting from the loss of heterozygosity at the TP53 locus. As the acquisition and expansion of cancer-associated mutations in hPS cells may go unnoticed during most applications, we suggest that careful genetic characterization of hPS cells and their differentiated derivatives be carried out before clinical use.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“and six specific variants in TP53 have been identified in several hPSC lines.165”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [165]
In the citing paper
“For pluripotent stem cells—embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs)—there appears to be a selection advantage for mutations of some oncogenes, in particular for TP53 and BCOR, which can become fixed in culture74,75.”
From How to design preclinical studies in nanomedicine and cell therapy to maximize the prospects of clinical translation (2018) · cites this paper as [74]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Robinson, J. T., Thorvaldsdóttir, H., Winckler, W., Guttman, M., Lander, E. S., Getz, G., & Mesirov, J. P. (2011) · Nature Biotechnology · doi:10.1038/nbt.1754 · Cited by 15,390
Kim, D., Pertea, G., Trapnell, C., Pimentel, H., Kelley, R., & Salzberg, S. L. (2013) · Genome Biology · doi:10.1186/gb-2013-14-4-r36 · Cited by 12,668
A framework for variation discovery and genotyping using next-generation DNA sequencing data
DePristo, M. A., Banks, E., Poplin, R. E., Garimella, K. V., Maguire, J., Hartl, C., Philippakis, A., del Angel, G., Rivas, M. A., Hanna, M., et al. (2011) · Nature Genetics · doi:10.1038/ng.806 · Cited by 11,457
Analysis of protein-coding genetic variation in 60,706 humans
Exome Aggregation Consortium, Lek, M., Karczewski, K. J., Minikel, E. V., Samocha, K. E., Banks, E., Fennell, T., O’Donnell-Luria, A. H., Ware, J. S., Hill, A. J., et al. (2016) · Nature · doi:10.1038/nature19057 · Cited by 9,587
Self-citationdbSNP: the NCBI database of genetic variation
Sherry, S. T. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.1.308 · Cited by 7,502
Vogelstein, B., Lane, D., & Levine, A. J. (2000) · Nature · doi:10.1038/35042675 · Cited by 6,607 · cited 1×
“(15) not recoverable after thaw (3) unavailable (9)”
BackgroundAge-related clonal hematopoiesis associated with adverse outcomes
Jaiswal, S., Fontanillas, P., Flannick, J., Manning, A., Grauman, P. V., Mar, B. G., Lindsley, R. C., Mermel, C. H., Burtt, N., Chavez, A., et al. (2014) · New England Journal of Medicine · doi:10.1056/nejmoa1408617 · Cited by 4,841
Clonal hematopoiesis and blood-cancer risk inferred from blood DNA sequence
Genovese, G., Kähler, A. K., Handsaker, R. E., Lindberg, J., Rose, S. A., Bakhoum, S. F., Chambert, K., Mick, E., Neale, B. M., Fromer, M., et al. (2014) · New England Journal of Medicine · doi:10.1056/nejmoa1409405 · Cited by 3,531
Self-citationHigh-throughput droplet digital PCR system for absolute quantitation of DNA copy number
Hindson, B. J., Ness, K. D., Masquelier, D. A., Belgrader, P., Heredia, N. J., Makarewicz, A. J., Bright, I. J., Lucero, M. Y., Hiddessen, A. L., Legler, T. C., et al. (2011) · Analytical Chemistry · doi:10.1021/ac202028g · Cited by 2,792
Crystal Structure of a p53 Tumor Suppressor-DNA Complex: Understanding Tumorigenic Mutations
Cho, Y., Gorina, S., Jeffrey, P. D., & Pavletich, N. P. (1994) · Science · doi:10.1126/science.8023157 · Cited by 2,559
Flagged first, then by citations
Cited by
Sorted by citations
Items 301–400 of 505
Martín, Á., Mercader, A., Beltrán, D., Mifsud, A., Nohales, M., Pardiñas, M. L., Ortega-Jaén, D., & Santos, M. J. D. L. (2024) · Human Reproduction · doi:10.1093/humrep/deae009 · Cited by 9
Potential Applications of Induced Pluripotent Stem Cells for Cardiovascular Diseases
Wang, X., Han, Z., Yu, Y., Xu, Z., Cai, B., & Yuan, Y. (2018) · Current Drug Targets · doi:10.2174/1389450120666181211164147 · Cited by 9
Transplanted stem cells survive a long time: do they make you sick?
Maguire, G. (2019) · Journal of the Royal Society of Medicine · doi:10.1177/0141076819851657 · Cited by 9
Leigh, R. S., Ruskoaho, H. J., & Kaynak, B. L. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02632-1 · Cited by 9
Fortschegger, K., Husa, A.-M., Schinnerl, D., Nebral, K., & Strehl, S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22147576 · Cited by 9
Precision genome-editing with CRISPR/Cas9 in human induced pluripotent stem cellsPreprint
Budde, J., Martinez, R. J., Hsu, S., Wen, N., Chen, J., Coppola, G., Goate, A., Cruchaga, C., & Karch, C. M. M. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/187377 · Cited by 9
Exploring the Tumor-Associated Risk of Mesenchymal Stem Cell Therapy in Veterinary Medicine
Jeung, S.-Y., Kim, S., Ah, J.-G., Seo, S., Jan, U., Lee, H., & Lee, J. I. (2024) · Animals · doi:10.3390/ani14070994 · Cited by 8
Nityanandam, A., Patton, M. H., Bayazitov, I. T., Newman, K. D., Thomas, K. T., & Zakharenko, S. S. (2025) · STAR Protocols · doi:10.1016/j.xpro.2025.103630 · Cited by 8
Huang, T., Radley, A., Yanagida, A., Ren, Z., Carlisle, F. A., Tahajjodi, S. S., Kim, D.-W., O’Neill, P., Clarke, J., Lancaster, M. A., et al. (2025) · Cell stem cell · doi:10.1016/j.stem.2025.02.002 · Cited by 8
Hypoimmune stem cells and islets: hype or a true breakthrough in diabetes treatment?
Shalaby, K. E., & Abdelalim, E. M. (2025) · Cellular & Molecular Biology Letters · doi:10.1186/s11658-025-00786-8 · Cited by 10
Xia, L., Qin, J., & Ye, S. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04826-0 · Cited by 10
Ye, M., Li, X., Chen, L., Mo, S., Liu, J., Huang, T., Luo, F., & Zhang, J. (2023) · Cancers · doi:10.3390/cancers15030592 · Cited by 10
The origins of human pluripotent stem cells: the road from a cancer to regenerative medicine
Andrews, P. W. (2024) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-024-00865-8 · Cited by 8
p53 Is Active in Human Amniotic Fluid Stem Cells
Rodrigues, M., Antonucci, I., Elabd, S., Kancherla, S., Marchisio, M., Blattner, C., & Stuppia, L. (2018) · Stem Cells and Development · doi:10.1089/scd.2017.0254 · Cited by 8
Xeno‐Free Reprogramming of Peripheral Blood Mononuclear Erythroblasts on Laminin‐521
Skorik, C., Mullin, N. K., Shi, M. A., Zhang, Y., Hunter, P., Tang, Y., Hilton, B. M., & Schlaeger, T. M. (2020) · Current Protocols in Stem Cell Biology · doi:10.1002/cpsc.103 · Cited by 8
Wu, J., & Barbaric, I. (2021) · Developmental Biology · doi:10.1016/j.ydbio.2021.03.025 · Cited by 8
Li, Q., Huang, Y., Xu, J., Mao, F., Zhou, B. O., Sun, L., Basinski, B. W., Aksu, M., Liu, J., Dou, Y., et al. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.10.002 · Cited by 8
Genomic Stability Testing of Pluripotent Stem Cells
McIntire, E. M., Taapken, S. M., Leonhard, K., & Larson, A. L. (2020) · Current Protocols in Stem Cell Biology · doi:10.1002/cpsc.107 · Cited by 10
Stavish, D., Price, C. J., Gelezauskaite, G., Leonhard, K. A., Taapken, S. M., McIntire, E. M., Laing, O., James, B. M., Riley, J. J., Zerbib, J., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.09.21.558777 · Cited by 8
Kim, Y., Kang, B., Kweon, S., Oh, S., Kim, D., Gil, D., Lee, H., Kim, J.-H., Ju, J. H., Roh, T.-Y., et al. (2024) · Experimental and Molecular Medicine · doi:10.1038/s12276-024-01334-8 · Cited by 8
Sarel-Gallily, R., Gunapala, K. M., & Benvenisty, N. (2025) · Stem Cell Reports · doi:10.1016/j.stemcr.2025.102471 · Cited by 7
Self-citationCell competition eliminates aneuploid human pluripotent stem cells
Ya, A., Deng, C., & Godek, K. M. (2025) · Stem Cell Reports · doi:10.1016/j.stemcr.2025.102506 · Cited by 7
iSCORE-PD: an isogenic stem cell collection to research Parkinson’s disease
Busquets, O., Li, H., Syed, K. M., Jerez, P. Á., Dunnack, J., Bu, R. L., Verma, Y., Pangilinan, G. R., Martin, A., Straub, J., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-74355-8 · Cited by 9
Manipulating cell fate while confronting reproducibility concerns
Osterloh, J. M., & Mullane, K. (2018) · Biochemical Pharmacology · doi:10.1016/j.bcp.2018.01.016 · Cited by 7
Donada, A., Basso‐Valentina, F., Arkoun, B., Monte-Mór, B. D. C. R., Plo, I., & Raslová, H. (2020) · Stem Cell Research · doi:10.1016/j.scr.2020.102060 · Cited by 7
de Lamotte, J. D., Perrier, A. L., Martinat, C., & Nicoleau, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22147524 · Cited by 7
Wnt signaling and the regulation of pluripotency
Sonavane, P. R., & Willert, K. (2023) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2023.01.002 · Cited by 8
Kim, J., Kwon, E., Kim, Y.-J., Kim, D., Shin, Y.-Z., Gil, D., Kim, J., Shin, H. D., Kim, L. H., Lee, M., et al. (2024) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-023-05060-8 · Cited by 6
Oscillatory Dynamics of p53-Mdm2 Circuit in Response to DNA Damage Caused by Ionizing Radiation
Zhang, Y., Liu, H., Li, Z., Miao, Z., & Zhou, J. (2019) · IEEE Transactions on Computational Biology and Bioinformatics · doi:10.1109/tcbb.2019.2899574 · Cited by 7
Montero, S. P., Bowling, S., Perez‐Carrasco, R., & Rodríguez, T. A. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.02.28.482311 · Cited by 7
Bai, X. J., Liu, T., Li, C., Qiu, C., Ge, X., Gou, H., Cai, H., Yang, L., Wei, S., Yang, W., et al. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04347-w · Cited by 6
p53 safeguards chemical reprogramming of human somatic cells toward pluripotency
Cheng, L., Wang, Y., Yang, Z., Cao, J., Peng, F., Lan, T., Cheng, R., Liu, T., Xia, Z., Guan, J., et al. (2026) · Cell · doi:10.1016/j.cell.2026.03.038 · Cited by 6
Modeling Maturity Onset Diabetes of the Young in Pluripotent Stem Cells: Challenges and Achievements
Braverman-Gross, C., & Benvenisty, N. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.622940 · Cited by 7
Self-citationCharacterizing the Genetic Stability of Human Naïve and Primed Pluripotent Stem Cells
Baker, D., & Barbaric, I. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1908-7_17 · Cited by 6
Optimizing CRISPR/Cas9 Editing of Repetitive Single Nucleotide Variants
Usher, I., Ligammari, L., Ahrabi, S., Hepburn, E., Connolly, C., Bond, G. L., Flanagan, A. M., & Cottone, L. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.932434 · Cited by 6
Single-cell mass cytometry analysis reveals stem cell heterogeneity
Meharwade, T., Joumier, L., Parisotto, M., & Malleshaiah, M. K. (2022) · Methods · doi:10.1016/j.ymeth.2022.09.009 · Cited by 6
Compromised Mitotic Fidelity in Human Pluripotent Stem Cells
Milagre, I., Pereira, C. A., & Oliveira, R. A. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241511933 · Cited by 6
Smela, M. P., Pepe, V., Lubbe, S. J., Kiskinis, E., & Church, G. M. (2024) · Stem Cell Reports · doi:10.1016/j.stemcr.2024.08.004 · Cited by 5
Quality control towards the application of induced pluripotent stem cells
Lin, K., & Xiao, A. (2017) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2017.07.006 · Cited by 7
A new approach to assess drug sensitivity in cells for novel drug discovery
Mazzarella, L., & Curigliano, G. (2018) · Expert Opinion on Drug Discovery · doi:10.1080/17460441.2018.1437136 · Cited by 6
Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools
Long, Y., & Cech, T. R. (2021) · Methods · doi:10.1016/j.ymeth.2021.01.002 · Cited by 6
Bialecka, M., Montilla‐Rojo, J., Roelen, B. A. J., Gillis, A., Looijenga, L. H. J., & Salvatori, D. C. F. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23094680 · Cited by 6
p53 Tumor Suppressor: Functional Regulation and Role in Gene Therapy
Farooq, Z., Wani, S., Ragunathrao, V. A. B., Kochhar, R. K., & Anwar, M. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.105029 · Cited by 7
Human Pluripotent Stem Cells: Genetic Instability or Stability?
Andrews, P. W. (2021) · Regenerative Medicine · doi:10.2217/rme-2021-0013 · Cited by 6
Cha, Y., Leblanc, P. R., & Kim, K. (2025) · Cell stem cell · doi:10.1016/j.stem.2025.05.005 · Cited by 5
Best Practice for Cell Banking Techniques Used in Cell Cultivated Products
Food Standards Agency, Strauss, B., Kleinsorge, B., & Lotfian, P. (2025) · FSA research and evidence. · doi:10.46756/001c.144239 · Cited by 5
Luo, Y., Xu, Z., & Li, Z. (2025) · FASEB BioAdvances · doi:10.1096/fba.2025-00126 · Cited by 5
Why in vivo models of disease remain indispensable
Bogani, D., Hooper, K. M., & Sansom, O. J. (2026) · Disease Models & Mechanisms · doi:10.1242/dmm.052997 · Cited by 5
Vidman, S. R., Ma, Y. H. E., Fullenkamp, N., & Plant, G. W. (2024) · Neural Regeneration Research · doi:10.4103/nrr.nrr-d-24-00901 · Cited by 6
Verheijen, B. M. (2019) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2019.00277 · Cited by 5
RetractedRath, P., Krämer, P., Biggs, D., Preece, C., Hortin, N., Diaz, R., Perez‐Alcantara, M., Li, X., Bolard, A., Beer, N. L., et al. (2025) · Stem Cells · doi:10.1093/stmcls/sxae092 · Cited by 7
Sebastian, S., Hourd, P. C., Chandra, A., Williams, D., & Medcalf, N. (2019) · Regenerative Medicine · doi:10.2217/rme-2018-0081 · Cited by 5
Chromosomal Instability and Karyotype Correction in Human Induced Pluripotent Stem Cells
Tv, N., Кашеварова, А. А., & Лебедев, И. Н. (2019) · Russian Journal of Genetics · doi:10.1134/s1022795419100090 · Cited by 5
Toward a better definition of hematopoietic progenitors suitable for B cell differentiation
Dubois, F., Gaignerie, A., Flippe, L., Heslan, J., Tesson, L., Chesneau, M., Haspot, F., Conchon, S., David, L., & Brouard, S. (2020) · PLoS ONE · doi:10.1371/journal.pone.0243769 · Cited by 5
Basinski, B. W., Balikov, D. A., Aksu, M., Li, Q., & Rao, R. C. (2021) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2021.01.001 · Cited by 5
Biological insights from the whole genome analysis of human embryonic stem cellsPreprint
Merkle, F. T., Ghosh, S. D., Genovese, G., Handsaker, R. E., Kashin, S., Karczewski, K. J., O’Dushlaine, C. T., Pato, C. N., Pato, M. T., MacArthur, D. G., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.10.26.337352 · Cited by 5
Self-citationZhang, H., del Barrio, J. L. A., Miguel, M. P. D., & Alió, J. L. (2025) · Experimental Eye Research · doi:10.1016/j.exer.2025.110631 · Cited by 4
Bell, M., & Krasnodembskaya, A. D. (2025) · Stem Cells Translational Medicine · doi:10.1093/stcltm/szaf040 · Cited by 4
Price, J. (2020) · The MIT Press eBooks · doi:10.7551/mitpress/10837.001.0001 · Cited by 4
Chen, Q., Zheng, J.-Y., & Wang, D.-C. (2025) · World Journal of Stem Cells · doi:10.4252/wjsc.v17.i2.103599 · Cited by 4
Single cell transcriptional perturbome in pluripotent stem cell models
Balmas, E., Ratto, M. L., Snijders, K. E., Becca, S., Liaci, C., Ricca, I., Merlo, G. R., Calogero, R., Alessandrì, L., Mendjan, S., et al. (2025) · Molecular Systems Biology · doi:10.1038/s44320-025-00172-8 · Cited by 4
Transcriptional Factors Mediated Reprogramming to Pluripotency
Fatima, N., Rahman, M. S. U., Qasim, M., Ashfaq, U. A., Ahmed, U., & Masoud, M. S. (2023) · Current Stem Cell Research & Therapy · doi:10.2174/1574888x18666230417084518 · Cited by 5
Mesfin, F. M., Manohar, K., Shelley, W. C., Brokaw, J. P., Liu, J., Ma, M., & Markel, T. A. (2023) · Seminars in Pediatric Surgery · doi:10.1016/j.sempedsurg.2023.151311 · Cited by 4
The Role of ATP-Binding Cassette Proteins in Stem Cell Pluripotency
Saini, P., Anugula, S., & Fong, Y. W. (2023) · Biomedicines · doi:10.3390/biomedicines11071868 · Cited by 4
Goyer, M., Désaulniers-Langevin, C., Sonn, A., Nehmo, G. M., Lisi, V., Benabdallah, B., Raynal, N. J. ‐., & Beausejour, C. M. (2024) · Cells · doi:10.3390/cells13100849 · Cited by 4
Zhang, Y., He, X., Ge, Z., Wang, B., Ni, M., & Cai, G. (2024) · Tissue and Cell · doi:10.1016/j.tice.2024.102499 · Cited by 4
Heart regeneration in mouse and human: a bioengineering perspective
Fine, B. M., & Vunjak‐Novakovic, G. (2020) · Current Opinion in Physiology · doi:10.1016/j.cophys.2020.01.004 · Cited by 4
ARHGDIA Confers Selective Advantage to Dissociated Human Pluripotent Stem Cells
Riggs, M. J., Sheridan, S. D., & Rao, R. R. (2021) · Stem Cells and Development · doi:10.1089/scd.2021.0079 · Cited by 4
CRISPR-Cas Gene Perturbation and Editing in Human Induced Pluripotent Stem Cells
van Essen, M. J., Riepsaame, J., & Jacob, J. (2021) · The CRISPR Journal · doi:10.1089/crispr.2021.0063 · Cited by 4
Assessing Cell Competition in Human Pluripotent Stem Cell (hPSC) Cultures
Price, C. J., & Barbaric, I. (2022) · Current Protocols · doi:10.1002/cpz1.435 · Cited by 4
Characterization of breakpoint regions of large structural autosomal mosaic events
Machiela, M. J., Jessop, L., Zhou, W., Yeager, M., & Chanock, S. J. (2017) · Human Molecular Genetics · doi:10.1093/hmg/ddx324 · Cited by 4
Le passage à l’échelle industrielle de la production de cellules souches à usage thérapeutique
Ardaillou, R., Jarry, B., Stoltz, J., Han, Z., Caen, J., Jarry, B., Jean-Emile, L., Daugéras, B., Bost, P., Ardaillou, R., et al. (2017) · Bulletin de l Académie Nationale de Médecine · doi:10.1016/s0001-4079(19)30420-0 · Cited by 4
Tutak, F. N., & Annaç, E. E. (2021) · Annals of Medicine and Surgery · doi:10.1016/j.amsu.2021.102861 · Cited by 4
Cell Therapy in Acute and Chronic Liver Disease
Paganelli, M. (2019) · Springer eBooks · doi:10.1007/978-3-319-96400-3_43 · Cited by 4
Stuppia, L., Cozene, B., & Antonucci, I. (2019) · Brain Circulation · doi:10.4103/bc.bc_35_19 · Cited by 4
Lee, J.-M., Lee, C., Seol, B., Jung, C. K., Kim, Y., Kang, D., Yu, H., Hong, Y., Song, C. L., Cho, Y. S., et al. (2025) · Experimental & Molecular Medicine · doi:10.1038/s12276-025-01439-8 · Cited by 4
Keshet, G., Barbaric, I., & Benvenisty, N. (2025) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2025.115743 · Cited by 4
Self-citationToward standardized iPSC testing: Insights from a multi-year international Quality Assessment Round
Hägg, A., Wood, R., Mochizuki, A. L., Abberton, K. M., Abranches, E., Álvarez-Palomo, B., Baptista, R., Barbosa, R. A. Q., BARRY, J. E. S., Carvalho, A. B., et al. (2026) · Stem Cell Reports · doi:10.1016/j.stemcr.2026.102857 · Cited by 3
Computational Analysis of Aneuploidy in Pluripotent Stem Cells
Weissbein, U. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9224-9_18 · Cited by 3
Genomic stability of mouse spermatogonial stem cells in vitro
Chuma, S., Kanatsu‐Shinohara, M., Katanaya, A., Hosokawa, M., & Shinohara, T. (2021) · Scientific Reports · doi:10.1038/s41598-021-03658-1 · Cited by 4
Lei, Y., Duong, M. C., Krivec, N., Janssens, C., Regin, M., Huyghebaert, A., de Deckersberg, E. C., Sermon, K., Delbany, D. A., & Spits, C. (2025) · Cell Proliferation · doi:10.1111/cpr.13813 · Cited by 3
Immunogenicity of autologous and allogeneic human primary cholangiocyte organoid cellular therapies
Petrus-Reurer, S., Lei, W., Tysoe, O., Mairesse, M., Baez‐Ortega, A., Jones, J. L., Tan, T., Rehakova, S., Mahbubani, K. T. A., Brodie, C. R., et al. (2025) · Cell Reports Medicine · doi:10.1016/j.xcrm.2025.102205 · Cited by 4
Jung, J., & Benvenisty, N. (2025) · Stem Cell Reports · doi:10.1016/j.stemcr.2025.102643 · Cited by 3
Self-citationNakashima, Y., Nahar, S., Miyagi‐Shiohira, C., Kinjo, T., Kobayashi, N., Saitoh, I., Watanabe, M., Fujita, J., & Noguchi, H. (2019) · Stem Cells International · doi:10.1155/2019/7274057 · Cited by 3
Suer, J. A. L., Sease, R., Hawkins, F. J., & Ryan, A. L. (2021) · European Respiratory Society eBooks · doi:10.1183/2312508x.10010120 · Cited by 3
Villarejo, L. G., Gerding, W. M., Bachmann, L., Hardt, L. H. I., Bormann, S., Nguyen, H. P., & Müller, T. (2024) · Cells · doi:10.3390/cells13060507 · Cited by 4
Assaying Cell Cycle Progression via Flow Cytometry in CRISPR/Cas9-Treated Cells
Geisinger, J. M., & Stearns, T. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1538-6_14 · Cited by 3
Sinha, S., Barbosa, K., Cheng, K., Leiserson, M. D. M., Wilson, D. M., Ryan, B. M., Ronai, Z. A., Lee, J. S., Deshpande, A. J., & Ruppin, E. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/407767 · Cited by 3
Garipler, G., Lu, C., Morrissey, A., López-Zepeda, L. S., Vidal, S. E., Aydın, B., Stadtfeld, M., Ohler, U., Mahony, S., Sanjana, N. E., et al. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.11.23.391771 · Cited by 3
Izadifar, M., Massumi, M., Prentice, K., Oussenko, T., Li, B., Elbaz, J., Puri, M., Wheeler, M. B., & Nagy, A. (2024) · Stem cell research & therapeutics · doi:10.1186/s13287-024-04059-7 · Cited by 2
Cost-effective non-additive GWAS across 2329 diseases in 500,349 individuals
Molotkov, I., Kurki, M., FinnGen, Palotie, A. V., Daly, M. J., & Artomov, M. (2025) · Nature Communications · doi:10.1038/s41467-025-67277-4 · Cited by 2
Zhang, K., Na, T., Jia, C., Yuan, X., Guo, M., Yang, X., Li, M., Jia, W., Bai, Z., Lu, J., et al. (2026) · Stem Cell Research & Therapy · doi:10.1186/s13287-026-04975-w · Cited by 3
Huo, Z., Tu, J. F., Yang, W., Huang, M.-F., Wang, R., Chu, C.-W., Xu, A., Yu, Y. T., Tavakol, T. N., Kizilbash, M., et al. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.111435 · Cited by 2
Stem Cell-Derived Insulin-Producing β Cells to Treat Diabetes
Harb, G., Poh, Y., & Pagliuca, F. W. (2017) · Current Transplantation Reports · doi:10.1007/s40472-017-0161-x · Cited by 2
Tutak, F. N., Doğan, F., & Annaç, E. E. (2021) · Burns · doi:10.1016/j.burns.2021.10.008 · Cited by 2
Replication-associated DNA damage in induced pluripotent stem cells
Vallabhaneni, H., & Hursh, D. A. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-99892-5.00023-2 · Cited by 2
Genome Editing of Pluripotent Stem Cells for Adoptive and Regenerative Cell Therapies
Blassberg, R. A. (2022) · GEN Biotechnology · doi:10.1089/genbio.2021.0010 · Cited by 3
Safety Issues Related to Pluripotent Stem Cell-Based Therapies: Tumour Risk
Hillenius, S., Montilla‐Rojo, J., Eleveld, T. F., Salvatori, D. C. F., & Looijenga, L. H. J. (2023) · Springer eBooks · doi:10.1007/978-3-031-41943-0_18 · Cited by 2
Kim, Y., Kim, S. M., Go, Y., Kim, H. S., Park, S., Kang, Y. P., & Cha, H.-J. (2024) · Biomaterials Research · doi:10.34133/bmr.0093 · Cited by 2
Emborg, M. E., Gambardella, J. C., Zhang, A., & Federoff, H. J. (2024) · Handbook of clinical neurology · doi:10.1016/b978-0-323-90120-8.00010-1 · Cited by 2
Metadata
- Authors
- Merkle, F. T., Ghosh, S., Kamitaki, N., Mitchell, J., Avior, Y., Mello, C., Kashin, S., Mekhoubad, S., Ilic, D., Charlton, M., Saphier, G., Handsaker, R. E., Genovese, G., Bar, S., Benvenisty, N., McCarroll, S. A., & Eggan, K.
- Affiliations
- Broad Institute (US)
- Hebrew University of Jerusalem (IL)
- Harvard University (US)
- Corresponding author
- McCarroll, Steven A.
- Eggan, Kevin
- ORCID
- Venue
- Nature
- Published
- 2017-04-26
- Volume
- 545
- Issue
- 7653
- Pages
- 229-233
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC5427175
- OpenAlex
- W2608500349
- Cited by
- 506
- References
- 44
- Citations by year
- 2026: 38 · 2025: 44 · 2024: 50 · 2023: 42 · 2022: 56 · 2021: 68 · 2020: 65 · 2019: 54 · 2018: 50 · 2017: 35
- FWCI
- 22.89
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- Research Portal (King's College London) (submittedVersion)
- Apollo (University of Cambridge) (submittedVersion)
- Apollo
- Funders
- Wellcome Trust
- Academy of Medical Sciences (SBF001\1016)
- Rosetrees Trust (M108-F1)
- National Institutes of Health (5K99NS08371)
- Medical Research Council (MC_PC_12009, MR/P501967/1)
- National Heart, Lung, and Blood Institute (R01HL109525)
- National Human Genome Research Institute (R01HG006855)
- National Institute of General Medical Sciences (P01GM099117)
- National Institute of Neurological Disorders and Stroke (K99NS083713)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- human pluripotent stem cells, human embryonic stem cells, induced pluripotent stem cells, TP53 mutation, dominant-negative p53, somatic mosaicism, copy number variation, clonal expansion, tumour suppressor, DNA-binding domain, stem cell culture conditions, exome sequencing, RNA sequencing
- MeSH terms
- Human Embryonic Stem Cells, Protein Domains, Alleles, Cell Count, Cell Differentiation, Cell Division, Cell Line, DNA, DNA Mutational Analysis, Genes, Dominant, Humans, Mosaicism, Mutation, Neoplasms, Selection, Genetic, Genes, p53, Tumor Suppressor Protein p53, Loss of Heterozygosity, Induced Pluripotent Stem Cells, Exome
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
