The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fusion protein
Open accessAbstract
RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we report an efficient siRNA delivery approach that uses a peptide transduction domain–double-stranded RNA-binding domain (PTD-DRBD) fusion protein. DRBDs bind to siRNAs with high avidity, masking the siRNA's negative charge and allowing PTD-mediated cellular uptake. PTD-DRBD–delivered siRNA induced rapid RNAi in a large percentage of various primary and transformed cells, including T cells, human umbilical vein endothelial cells and human embryonic stem cells. We observed no cytotoxicity, minimal off-target transcriptional changes and no induction of innate immune responses. Thus, PTD-DRBD–mediated siRNA delivery allows efficient gene silencing in difficult-to-transfect primary cell types.
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Cites
Knocking down barriers: advances in siRNA delivery
Whitehead, K. A., Langer, R., & Anderson, D. G. (2009) · Nature Reviews Drug Discovery · doi:10.1038/nrd2742 · Cited by 3,115
Wadia, J. S., Stan, R. V., & Dowdy, S. F. (2004) · Nature Medicine · doi:10.1038/nm996 · Cited by 1,647
Self-citationDevelopment of a self-inactivating lentivirus vector
Miyoshi, H., Blömer, U., Takahashi, M., Gage, F. H., & Verma, I. M. (1998) · Journal of Virology · doi:10.1128/jvi.72.10.8150-8157.1998 · Cited by 1,196
Safran, M., Kim, W. Y., Kung, A. L., Horner, J. W., Depinho, R. A., & Kaelin, W. G. (2003) · Molecular Imaging · doi:10.1162/153535003322750637 · Cited by 134
Cited by
The delivery of therapeutic oligonucleotides
Juliano, R. L. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw236 · Cited by 854
Silencing disease genes in the laboratory and the clinic
Watts, J. K., & Corey, D. R. (2012) · The Journal of Pathology · doi:10.1002/path.2993 · Cited by 470
Demoor, M., Ollitrault, D., Gomez-Leduc, T., Bouyoucef, M., Hervieu, M., Fabre, H., Lafont, J., Denoix, J.-M., Audigié, F., Mallein-Gerin, F., et al. (2014) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2014.02.030 · Cited by 296
Versatile RNA Interference Nanoplatform for Systemic Delivery of RNAs
Choi, K. Y., Silvestre, O. F., Huang, X., Min, K. H., Howard, G. P., Hida, N., Jin, A. J., Carvajal, N., Lee, S. W., Hong, J.-I., et al. (2014) · ACS Nano · doi:10.1021/nn500085k · Cited by 104
Directing human embryonic stem cell differentiation by non-viral delivery of siRNA in 3D culture
Zoldan, J., Lytton-Jean, A. K. R., Karagiannis, E. D., Deiorio-Haggar, K., Bellan, L. M., Langer, R., & Anderson, D. G. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.06.057 · Cited by 47
Metadata
- Authors
- Eguchi, A., Meade, B. R., Chang, Y.-C., Fredrickson, C. T., Willert, K., Puri, N., & Dowdy, S. F.
- Venue
- Nature Biotechnology
- Published
- 2009-05-17
- Volume
- 27
- Issue
- 6
- Pages
- 567-571
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2084621409
- Cited by
- 340
- References
- 4
- FWCI
- 13.56
- Type
- journal article
- Language
- en
- Open access
- green
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- Eguchi, A., Meade, B. R., Chang, Y.-C., Fredrickson, C. T., Willert, K., Puri, N., & Dowdy, S. F. (2009). Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fusion protein. Nature Biotechnology. https://doi.org/10.1038/nbt.1541
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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