Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that Have Undergone Cre-Mediated Recombination
Open accessAbstract
Conditional alleles containing LoxP recombination sites, in conjunction with Cre recombinase delivered by a variety of means, allows for spatial and temporal control of gene expression in mouse models. Here we describe a mouse strain in which a luciferase (Luc) cDNA, preceded by a LoxP-stop-LoxP (L-S-L) cassette, was introduced into the ubiquitously expressed ROSA26 locus. Mouse embryo fibroblasts derived from this strain expressed luciferase after Cre-mediated recombination in vitro. ROSA26 L-S-L-Luc/+ mice expressed luciferase in a diffuse or liver-restricted pattern, as determined by noninvasive, bioluminescent imaging, when crossed to transgenic mice in which Cre was under the control of a zygotically expressed (EIIA-Cre), or a liver-restricted (albumin-Cre), promoter, respectively. Organ-specific luciferase expression was also seen after intraparenchymal administration of an adenovirus encoding Cre. The ROSA26 L-S-L-Luc/+ strain should be useful for characterizing Cre mouse strains and for following the fate of cells that have undergone Cre-mediated recombination in vivo.
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The FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J mouse line contains a luciferase open reading frame inserted into the ROSA26 locus.
“The FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J mouse line contains a luciferase open reading frame inserted into the ROSA26 locus.23”
From Adeno-Associated Virus Serotype-Specific Inverted Terminal Repeat Sequence Role in Vector Transgene Expression (2020) · cites this paper as [23]
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- Authors
- Safran, M., Kim, W. Y., Kung, A. L., Horner, J. W., Depinho, R. A., & Kaelin, W. G.
- Affiliations
- Brigham and Women's Hospital (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Kaelin, William G.
- Venue
- Molecular Imaging
- Publisher
- SAGE Publications
- Published
- 2003-10-01
- Volume
- 2
- Issue
- 4
- Pages
- 297-302
- ISSN
- 1535-3508 (ISSN-L)
- 1536-0121
- OpenAlex
- W2125319175
- Cited by
- 134
- References
- 0
- Citations by year
- 2025: 2 · 2024: 6 · 2023: 4 · 2022: 2 · 2021: 5 · 2020: 9 · 2019: 7 · 2018: 11 · 2017: 7 · 2016: 6 · 2015: 6 · 2014: 9 · 2013: 11 · 2012: 9
- FWCI
- 1.15
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Molecular Imaging (publishedVersion)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- Cre-mediated recombination, conditional alleles, Rosa26 locus, luciferase reporter, bioluminescence imaging, non-invasive imaging, cell fate tracking
- MeSH terms
- Adenoviridae, Albumins, Animals, Brain, Fibroblasts, Genetic Engineering, Kidney, Liver, Luciferases, Luminescent Measurements, Mice, Transgenic, Organ Specificity, Promoter Regions, Genetic, Recombination, Genetic, Viral Proteins, Genes, Reporter, Integrases, Mice
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