The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer
Open accessAbstract
Comprehensive analyses of cancer genomes promise to inform prognoses and precise cancer treatments. A major barrier, however, is inaccessibility of metastatic tissue. A potential solution is to characterize circulating tumor cells (CTCs), but this requires overcoming the challenges of isolating rare cells and sequencing low-input material. Here we report an integrated process to isolate, qualify and sequence whole exomes of CTCs with high fidelity using a census-based sequencing strategy. Power calculations suggest that mapping of >99.995% of the standard exome is possible in CTCs. We validated our process in two patients with prostate cancer, including one for whom we sequenced CTCs, a lymph node metastasis and nine cores of the primary tumor. Fifty-one of 73 CTC mutations (70%) were present in matched tissue. Moreover, we identified 10 early trunk and 56 metastatic trunk mutations in the non-CTC tumor samples and found 90% and 73% of these mutations, respectively, in CTC exomes. This study establishes a foundation for CTC genomics in the clinic.
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Cites
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Scalable whole-exome sequencing of cell-free DNA reveals high concordance with metastatic tumors
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Self-citationGenome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
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Metadata
- Authors
- Lohr, J. G., Adalsteinsson, V. A., Cibulskis, K., Choudhury, A. D., Rosenberg, M., Cruz-Gordillo, P., Francis, J. M., Zhang, C.-Z., Shalek, A. K., Satija, R., Trombetta, J. J., Lu, D., Tallapragada, N., Tahirova, N., Kim, S., Blumenstiel, B., Sougnez, C., Lowe, A., Wong, B., Auclair, D., Van Allen, E. M., Nakabayashi, M., Lis, R. T., Lee, G.-S. M., Li, T., Chabot, M. S., Ly, A., Taplin, M.-E., Clancy, T. E., Loda, M., Regev, A., Meyerson, M., Hahn, W. C., Kantoff, P. W., Golub, T. R., Getz, G., Boehm, J. S., & Love, J. C.
- Venue
- Nature Biotechnology
- Published
- 2014-04-20
- Volume
- 32
- Issue
- 5
- Pages
- 479-484
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2120227082
- Cited by
- 535
- References
- 14
- FWCI
- 24.59
- Type
- journal article
- Language
- en
- Open access
- green
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- Lohr, J. G., Adalsteinsson, V. A., Cibulskis, K., Choudhury, A. D., Rosenberg, M., Cruz-Gordillo, P., Francis, J. M., Zhang, C.-Z., Shalek, A. K., Satija, R., Trombetta, J. J., Lu, D., Tallapragada, N., Tahirova, N., Kim, S., Blumenstiel, B., Sougnez, C., Lowe, A., Wong, B., . . . Love, J. C. (2014). Whole-exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer. Nature Biotechnology. https://doi.org/10.1038/nbt.2892
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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