Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

Cited by (1,000)

Items 901–1,000 of 1,000

  1. Target residence of Cas9: challenges and opportunities in genome editing
    Yi-Li Feng, Meng Wang, Anyong Xie · 2022 · Genome Instability & Disease
  2. Gene editing treatment strategies for retinitis pigmentosa assessed in Xenopus laevis carrying a mutant Rhodopsin allele
    Farhad Ghaseminejad, Beatrice M. Tam, Colette N. Chiu, Joanna M. Feehan, Orson L. Moritz · 2022 · Journal of Translational Genetics and Genomics
  3. Recent advances in the application of induced pluripotent stem cell technology to the study of myeloid malignancies
    Dharamveer Tatwavedi, Andrea Pellagatti, Jacqueline Boultwood · 2023 · Advances in Biological Regulation
  4. Advances in CRISPER/Cas system and genome editing technologies for the treatment of neurodegenerative diseases
    Shailendra Kumar Mishra, Hung‐Ming Chang, Ahmad A. Obaid, Sandeep Kumar Singh · 2024 · Elsevier eBooks
  5. Efficient CRISPR/Cas9 Knock‐in Approaches for Manipulation of Endogenous Genes in Human B Lymphoma Cells
    Laura A. Murray‐Nerger, Benjamin E. Gewurz · 2024 · Current Protocols
  6. Bioethical and practical aspects of experimentation with zebrafish embryos and adults
    Magda Regina Santiago, Mariana Sandes Félix · 2024 · Arquivos do Instituto Biológico
  7. CRISPR/Cas9 Ribonucleoprotein Delivery Enhanced by Lipo-Xenopeptide Carriers and Homology-Directed Repair Modulators: Insights from Reporter Cell Lines
    Xianjin Luo, Eric Weidinger, Tobias Burghardt, Miriam Höhn, Ernst Wagner · 2025 · International Journal of Molecular Sciences
  8. High-Frequency Generation of Homozygous/Biallelic Mutants via CRISPR/Cas9 Driven by AtKu70/80 Promoters
    Huihui Zhang, Chong Teng, Shanhua Lyu, Yinglun Fan · 2025 · International Journal of Molecular Sciences
  9. Genetic engineering as a tool for the generation of mouse models to understand disease phenotypes and gene function
    Ann‐Kathrin Baumgart, Marc Beyer · 2017 · Current Opinion in Biotechnology
  10. Modelling AKI in vitro: taking organoids to the next level
    Letizia De Chiara, Yun Fei Xia · 2022 · Kidney International
  11. Increase of c-FOS promoter transcriptional activity by the dual leucine zipper kinase
    Kyra-Alexandra Köster, Jorge Duque Escobar, Anja Fietkau, Regina Toledo, Elke Oetjen · 2023 · Naunyn-Schmiedeberg s Archives of Pharmacology
  12. DNA-dependent protein kinase inhibitors PI-103 and samotolisib augment CRISPR/Cas9 knock-in efficiency in human T cells
    Emina Džafo, Morteza Hafezi, Greta Maria Paola Giordano Attianese, Patrick Reichenbach, Stéphane Grillet, Hélène Garcia, Elisabetta Cribioli, Christel Voize, Stéphanie Tissot-Renaud, Romain Vuillefroy de Silly et al. · 2025 · Cytotherapy
  13. Recombinant Adeno-Associated Virus Vector Mediated Gene Editing in Proliferating and Polarized Cultures of Human Airway Epithelial Cells
    Soo Yeun Park, Zehua Feng, Soon Ho Choi, Xiujuan Zhang, Yinghua Tang, Grace N. Gasser, Donovan Richart, Feng Yuan, Jianming Qiu, John F. Engelhardt et al. · 2025 · Human Gene Therapy
  14. CRISPR-Cas9 HDR optimization: RAD52, denatured, and 5′-modified DNA templates in knock-in mice generation
    Boris V. Skryabin, Daniela Anne Braun, Helena Kaiser, Leonid Gubar, Birte Seeger, Tasneem Khanam, Anja Stegemann, Hermann Josef Pavenstädt, Timofey S. Rozhdestvensky · 2025 · iScience
  15. Experimental approaches to studying the nature and impact of splicing variation in zebrafish
    Maria-Cristina Keightley, Sebastian Markmiller, Christopher G. Love, John E.J. Rasko, Graham John Lieschke, Joan K. Heath · 2016 · Methods in cell biology
  16. OUP accepted manuscript
    Selma Souf · 2016 · Bioscience Horizons The International Journal of Student Research
  17. The transformational impact of site-specific DNA modifiers on biomedicine and agriculture
    Kathryn M. Polkoff, Jorge A. Piedrahita · 2018 · Animal Reproduction
  18. Development of Cellular Models to Study Efficiency and Safety of Gene Edition by Homologous Directed Recombination Using the CRISPR/Cas9 System
    Sabina Sánchez-Hernández, Araceli Aguilar-González, Beatriz Guijarro-Albaladejo, Noelia Maldonado‐Pérez, Iris Ramos-Hernández, Marina Cortijo-Gutierréz, Rosario Maria Sanchez-Martin, Karim Benabdellah, Francisco Martı́n · 2020 · Cells
  19. Computational Approaches for Designing Highly Specific and Efficient sgRNAs
    Jaspreet Kaur Dhanjal, Dhvani Sandip Vora, Navaneethan Radhakrishnan, Durai Sundar · 2021 · Methods in molecular biology
  20. Homologous Recombination-Enhancing Factors Identified by Comparative Transcriptomic Analyses of Pluripotent Stem Cell of Human and Common Marmoset
    Sho Yoshimatsu, Mayutaka Nakajima, Emi Qian, Tsukasa Sanosaka, Tsukika Sato, Hideyuki Okano · 2022 · Cells
  21. Targeted Gene Replacement in Plants Using CRISPR-Cas Technology
    Wei Zheng, Rukmini Mishra, Raj Kumar Joshi, Kaijun Zhao · 2022 · Springer eBooks
  22. Nebulous without white: annotated long-read genome assembly and CRISPR/Cas9 genome engineering in Drosophila nebulosa
    Christopher J. Sottolano, Nicole T. Revaitis, Anthony J. Geneva, Nir Yakoby · 2022 · G3 Genes Genomes Genetics
  23. Recent Advances in In Vivo Genome Editing Targeting Mammalian Preimplantation Embryos
    Masahiro Sato, Masato Ohtsuka, Emi Inada, Shingo Nakamura, Issei Saitoh, Shuji Takabayashi · 2022 · IntechOpen eBooks
  24. Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering
    Qianxin Wu, Yale S. Michaels, Tudor A. Fulga · 2023 · Methods in molecular biology
  25. Time-lapse imaging of primary cilium behavior with physiological expression of fluorescent ciliary proteins
    Ryota Nakazato, Hiroshi Otani, Faryal Ijaz, Koji Ikegami · 2023 · Methods in cell biology
  26. CATI: an efficient gene integration method for rodent and primate embryos by MMEJ suppression
    Hongyu Chen, Xingchen Liu, Lanxin Li, Qingtong Tan, Shiyan Li, Li Li, Chunyang Li, Jiqiang Fu, Yong Lu, Yan Wang et al. · 2023 · Genome biology
  27. An efficient evaluation system for factors affecting the genome editing efficiency in mouse
    Yusuke Sakai, Yuri Okabe, Gen Itai, Seiji Shiozawa · 2023 · EXPERIMENTAL ANIMALS
  28. Genetic Pest Management Technologies to Control Invasive Rodents.
    Dona Kanavy · 2018 · NCSU Libraries Repository (North Carolina State University Libraries)
  29. Maximizing the Efficacy of CRISPR/Cas Homology-Directed Repair Gene Targeting
    Terry S. Elton, Md. Ismail Hossain, Jessika Carvajal‐Moreno, Xinyi Wang, Dalton J. Skaggs, Jack C. Yalowich · 2022 · IntechOpen eBooks
  30. “Ancestralization” of human pluripotent stem cells by multiplexed precise genome editing
    Stephan Riesenberg, Tomislav Maričić, Svante Pääbo · 2018 · bioRxiv (Cold Spring Harbor Laboratory)
  31. Age-related cardiopathies gene editing
    Moshi Song, Juan Carlos Izpisúa Belmonte, Guanghui Liu · 2019 · Aging
  32. Generating Nonmosaic Mutants in Xenopus Using CRISPR–Cas in Oocytes
    Sang‐Wook Cha · 2021 · Cold Spring Harbor Protocols
  33. Rational design of Cas9 ribonucleoprotein with a “gRNA-shRNA” for multidimensional genome manipulation and enhanced homology-directed repair
    Jie Qiao, Wenli Sun, Wenhao Yin, Lixin Ma, Yi Liu · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
  34. Comprehensive analysis of end-modified long dsDNA donors in CRISPR-mediated endogenous tagging
    Rioka Takagi, Shoji Hata, Chiharu Tei, Akira Mabuchi, Ryosuke Anzai, Masamitsu Fukuyama, Shohei Yamamoto, Takumi Chinen, Atsushi Toyoda, Daiju Kitagawa · 2024 · bioRxiv (Cold Spring Harbor Laboratory)
  35. Current status and perspectives of gene therapy in the treatment of haematological cancers
    Yuhan Sun · 2023 · Theoretical and Natural Science
  36. A Recombinase-Mediated Cassette Exchange Platform for a Triple Independent Inducible Expression System for Human Pluripotent Stem Cells
    Roberto Castro-Gutiérrez, Ankita Arora, Katherine F. Vaeth, J. Matthew Taliaferro, Holger A. Russ · 2025 · Cells
  37. Knockout of Ligase4 gene enhances homology‐directed repair efficiency in Plutella xylostella
    Faisal Munir, Uroosa Zaheer, Sheng Lin, Anam Noreen Abbas, Mubashir Tariq, Jianying Liao, Qingxuan Qiao, Yanpeng Chang, Yussuf Mohamed Salum, Ibrahim Adams et al. · 2025 · Insect Science
  38. CRISPR/Cas9-integrated nanocarriers for cancer gene therapy: Mechanistic insights, challenges, and clinical translation
    Abhay Thakur, Rahul Sharma, Rohit Sharma, Kamal Jeet, Amar Deepak · 2025 · Journal of Drug Delivery Science and Technology
  39. Therapeutic Gene Editing: DNA Repair Pathways, Emerging Editors, and Clinical Progress
    Li‐Kuang Tsai, Youcai Xiong, Dongqi Liu, Dongshan Yang, Jifeng Zhang, Jie Xu, Renzhi Han, YiChen Chen · 2026 · iNew Medicine
  40. Circumvention of acquired resistance to topoisomerase II-targeted anticancer agents in HL-60 leukemia cells by prevention of intronic polyadenylation
    Xinyu Zhao, Sydney R Cassel, Sarah Eaton, Junan Li, Xinyi Wang, Terry S. Elton, Jack C. Yalowich · 2026 · Journal of Pharmacology and Experimental Therapeutics
  41. Gene-Edited Cell Models to Study Chronic Wasting Disease
    Simrika Thapa, Cristóbal Marrero-Winkens, Waqas Tahir, Maria Immaculata Arifin, Sabine Gilch, Hermann M. Schätzl · 2022 · Viruses
  42. Increasing Knockin Efficiency in Mouse Zygotes by Transient Hypothermia
    Amine Bouchareb, Daniel Biggs, Samy Alghadban, Christopher Preece, Benjamin Davies · 2024 · The CRISPR Journal
  43. Engineering single-cycle MeV vector for CRISPR-Cas9 gene editing
    Ramya Rallabandi, Brenna Sharp, Spencer Majerus, Austin Royster, Sarrianna Hoffer, Mia Ikeda, Patricia Devaux · 2024 · Molecular Therapy — Methods & Clinical Development
  44. The powers and perils of bug editing
    Helena Marcolla Araujo · 2024 · Insect Biochemistry and Molecular Biology
  45. 3209 – ENHANCING KNOCK-IN EFFICIENCY IN HSCs BY MODULATING THE DNA DAMAGE RESPONSE VIA ATM INHIBITION
    Munkh‐Erdene Natsagdorj, Hiromasa Hara, Kohei Iida, Yuji Kashiwakura, Tsukasa Ohmori, Yutaka Hanazono, Fumio Nakahara, Sandra Pinho, Luigi Naldini, Tsukasa Ohmori et al. · 2025 · Experimental Hematology
  46. HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects
    Yu Shirai, Jonchee A. Kao, Tarun Kumar, Naoki Matsuda, Ryoya Nakagawa, Osamu Nureki, Cassandra G. M. Extavour, Takaaki Daimon · 2026 · Communications Biology
  47. The future of pediatric gene therapy: CRISPR-Cas9, AI, and personalized medicine
    Yinping Pan, Jian Ding, W Wang, Dexin Xu, Jihuan Fan, Wenbin Zhang · 2026 · Pediatric Research
  48. Donor-complementary prime editing enables precise kilobase and library-compatible DNA insertions
    Yunzheng Fang, Jingyao Tang, Jiawei Xi, Binfeng Yang, Feng Zhang, Lingbo Wang · 2026 · Nature Biotechnology
  49. Progress in Gene Editing Transgenesis Genome Manipulation in Mosquitoes
    Justin M. Overcash, Zach N. Adelman · 2016 · Advances in insect physiology
  50. Genome engineering in human pluripotent stem cells
    Udit Parekh, Marianna Yusupova, Prashant Mali · 2017 · Current Opinion in Chemical Engineering
  51. CRISPR Highlights and Transition of Cas9 into a Genome Editing Tool
    Thomas Bentin · 2018 · Royal Society of Chemistry eBooks
  52. Research progress on non-viral vectors of CRISPR/Cas9 system
    Qi Liu, Zhanzhan Zhang, Yang Liu · 2019 · Scientia Sinica Chimica
  53. DNA templates with blocked long 3ʹ end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci
    Saptaparni Bandyopadhyay, Joseph Douglass, Sebastian Kapell, Nazimuddin Khan, Fabiana Feitosa-Suntheimer, Jenny A. Klein, Jasmine Temple, Jayce Brown-Culbertson, Alexander H. Tavares, Mohsan Saeed et al. · 2021 · G3 Genes Genomes Genetics
  54. High-throughput navigation of the sequence space
    Alaksh Choudhury · 2022 · Elsevier eBooks
  55. Genome Editing in Human Pluripotent Stem Cells
    Liuhong Cai, Yoon-Young Jang, Zhaohui Ye · 2016 · Stem cell biology and regenerative medicine
  56. Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing
    Syed Mukhtar Ahmed · 2022 · Methods in molecular biology
  57. CRISPR Technology - Recent Advances
    Yuan‐Chuan Chen · 2022 · IntechOpen eBooks
  58. Genome Editing and Pathological Cardiac Hypertrophy
    Takao Kato · 2022 · Advances in experimental medicine and biology
  59. Genome Editing: A Review of the Challenges and Approaches
    Dimple Sharma, Harmanpreet Kaur, Harsimran Kaur Kapoor, Rajat Sharma, Harpreet Kaur, Mohd Kyum · 2022 · Springer eBooks
  60. Cadmium chloride down-regulates the expression of Rad51 in HC11 cells and reduces knock-in efficiency
    Ga-Yeon Kim, Man‐Jong Kang · 2023 · Journal of Animal Reproduciton and Biotechnology
  61. Gene Therapy and Gene Editing
    Niloofar Bazazzadegan, Mandana Hasanzad, Seyedeh Sedigheh Abedini · 2024 · Springer eBooks
  62. Possible Application of GONAD/i-GONAD, a Novel In Vivo Genome Editing Technology, to Generate Genome-edited Large Experimental Animals
    Satoshi Watanabe, Shuji Takabayashi, Kazunori Morohoshi, Shingo Nakamura, Masahiro Sato · 2024 · Nature Cell and Science
  63. Synergistic effect of asciminib with reduced doses of ponatinib in human Ph + myeloid leukemia with the T315M mutation
    Thao Nguyen, Daisuke Harama, Minori Tamai, Keiko Kagami, Chiaki Komatsu, Shin Kasai, Koshi Akahane, Kumiko Goi, Takeshi Inukai · 2025 · International Journal of Hematology
  64. CRISPR/Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells
    Jianping Zhang, Amanda Neises, Tao Cheng, Xiao‐Bing Zhang · 2015 · SpringerBriefs in stem cells
  65. Using Engineered Nucleases to Create HIV-Resistant Cells
    George N. Llewellyn, Colin M. Exline, Nathalia G. Holt, Paula M. Cannon · 2016 · Advances in experimental medicine and biology
  66. Genome-Editing Technology in CRISPR/Cas System: How to Increase Knock-In Efficiency in Mouse Zygotes
    Takuro Horii, Izuho Hatada · 2016 · Springer eBooks
  67. Strategy for applying CRISPR/Cas9 gene editing technology in neuroscience
    QiPeng ZHANG, Anyang Sun · 2017 · Chinese Science Bulletin (Chinese Version)
  68. CRISPR/Cas9系统: 一种强大的在体基因治疗工具
    大力 李, 明耀 刘, 立人 王, 晓辉 张 · 2017 · Scientia Sinica Vitae
  69. CRISPR/Cas9-Mediated Genome Nano-Surgery: An Update
    Kinan Mokbel, Kefah Mokbel · 2017 · Biochemistry & Molecular Biology Journal
  70. Engineering of Human-Induced Pluripotent Stem Cells for Precise Disease Modeling
    Paweł Lisowski · 2018 · Springer eBooks
  71. MultiSite Gateway Technology Is Useful for Donor DNA Plasmid Construction in CRISPR/Cas9-Mediated Knock-In System
    Takeshi Yasuda, Katsushi Tajima · 2019 · IntechOpen eBooks
  72. Targeting the diapause response of Aedes albopictus via CRISPR/Cas9-mediated genome editing to control the spread of mosquito-borne disease
    Zachory M. Park, Amy M. Lyndaker · 2018 · BIOS
  73. CRISPR-based Technologies for Genome Engineering: Properties, Current Improvements and Applications in Medicine
    Sylvain Geny, Elaheh Sadat Hosseini, Jean‐Paul Concordet, Carine Giovannangeli · 2019 · Royal Society of Chemistry eBooks
  74. Research and applications of genetics-based methods for pest control
    Weiyi He, Wei Chen, Minsheng You, Jie Yang, Xuejiao Xu · 2019 · Scientia Sinica Vitae
  75. Histone deacetylases inhibitor and RAD51 recombinase increase transcription activator-like effector nucleases-mediated homologous recombination on the bovine β-casein gene locus
    Da Som Park, Se Eun Kim, Deog‐Bon Koo, Man‐Jong Kang · 2019 · Asian-Australasian Journal of Animal Sciences
  76. USE OF GENOME EDITING TECHNOLOGIES: ACHIEVEMENTS AND FURURE PROSPECTS
    А. А. Мохов, А. А. Чапленко, A.N. Yavorskiy · 2019 · Journal Biomed
  77. Characterization of Substitution Mutations of eIF4G Gene Generated through Adenine Base Editors in Rice
    Yaiphabi Kumam, C Rajadurai, K. K. Kumar, S. Varanavasiappan, M Raveendran, S. Manonmani, Chandrasekhar Gopalakrishnan, Loganathan Arul, Eswaran Kokiladevi, D. Sudhakar · 2021 · Madras Agricultural Journal
  78. Delivery Methods for CRISPR/Cas Reagents
    Sidra Ashraf, Nayla Munawar, Muhammad Kashif Zahoor, Amer Jamil, Masooma Hammad, Abdul Ghaffar, Aftab Ahmad · 2022 · Springer eBooks
  79. Efficient Large DNA Fragment Knock-in by Long dsDNA with 3′-Overhangs Mediated CRISPR Knock-in (LOCK) in Mammalian Cells
    Wenjie Han, Haojun Liang, Jianqiang Bao · 2023 · BIO-PROTOCOL
  80. CRISPR-Cas9 in gene therapy: much control on breaking, little control on repairing
    Kaveh Daneshvar · 2015
  81. Modulation of Genome Editing Outcomes by Cell Cycle Control of Cas9 Expression
    Yuping Huang, Caitlin McCann, Andrey Samsonov, Dmitry Malkov, Gregory D. Davis, Qingzhou Ji · 2017 · bioRxiv (Cold Spring Harbor Laboratory)
  82. “Global Transcriptional Response to CRISPR/CAS9-AAV6 Based Genome Editing” Matches Transcriptional Response to Specific Small Molecule Perturbations
    Akpéli V. Nordor, Martin J. Aryee, Geoffrey Siwo · 2018 · bioRxiv (Cold Spring Harbor Laboratory)
  83. A CyclinB2-Cas9 fusion promotes the homology-directed repair of double-strand breaks
    Manuel M. Vicente, Afonso Mendes, M. Jason de la Cruz, José R. Vicente, Vasco M. Barreto · 2019 · bioRxiv (Cold Spring Harbor Laboratory)
  84. Therapeutic approaches and development of genomic diagnostic tools for Usher syndrome
    Carla Fuster García · 2020 · RiuNet (Universitat Politècnica de València)
  85. Nonhomologous DNA End Joining in Mammalian Cells
    Ujjayinee Ray, Sathees C. Raghavan · 2022 · Elsevier eBooks
  86. Inhibiting POLQ-mediated alternative NHEJ enhances CRISPR/Cas9 mediated precise genome editing in CHO cells
    Chuanjie Wang, Ming Wang, Mengmeng Zhang, Yao Wang, Xinying Li, Chenghua Liu, Rongrong Fan, Yuanqiang Zheng, Beifen Shen, Zhaolin Sun et al. · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
  87. Gene editing approaches of microsatellite disorders : shortening expanded repeats
    Lucie Poggi · 2020 · theses.fr (ABES)
  88. A bulk cell heterozygous knock-in strategy for targeted protein degradation
    Beibei Liu, Chao Qi, Tomoharu Kanie · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
  89. Barriers to Effective Genome Editing of Haematopoietic Stem Cells
    Steven J. Howe, Michael D. Milsom · 2016 · Current Stem Cell Reports
  90. Recent Advances in the Enabling Technologies for Synthetic Biology
    Yinhua Lü, Xiaoli Xue, Lei LI, Zhongjun Qin, Weihong Jiang · 2015 · Scientia Sinica Vitae
  91. GMO Acceptance in the World and Issues for the Overcoming of Restrictions
    Palmiro Poltronieri · 2016 · Elsevier eBooks
  92. Generation of Tissue-Specific Mouse Models to Analyze HDAC Functions
    Astrid Hagelkrüys, Mirjam Andrea Moser, Christian Seiser · 2016 · Methods in molecular biology
  93. CRISPR ‐Cas Systems for the Study of Immune Function
    S. William Pelletier · 2016 · Wiley eBooks
  94. Precision engineering of the chicken genome for disease control
    Arjun Challagulla · 2016
  95. Enhancing Neuronogenesis and Counteracting Neuropathogenic Gene Haploinsufficiencies by RNA Gene Activation
    Antonello Mallamaci · 2017 · Advances in experimental medicine and biology
  96. The potential of Genome-editing techniques
    Tsukasa Ohmori · 2015 · Japanese Journal of Thrombosis and Hemostasis
  97. PRO136ALA CTLA4 Mutation
    C.N.A. Palmer · 2017 · Digital Access to Scholarship at Harvard (DASH) (Harvard University)
  98. Development of Site-Specific CFTR Gene Integration Tools for Testing Gene Editing in Pig Cells
    Liang Yang · 2017 · TSpace (University of Toronto)
  99. CRISPR/Cas9技术在生物医学领域的非病毒及病毒载体
    舟 秦, 可 门, 珽 徐, 治尧 何, 于全 魏, 阳 杨 · 2017 · Scientia Sinica Vitae
  100. The role of wound healing in oral health
    Marcela M. Fernandez-Gutierrez · 2018