The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR–Cas9 library
Open accessAbstract
CRISPR–Cas9 screens have been widely adopted to analyze coding-gene functions, but high-throughput screening of non-coding elements using this method is more challenging because indels caused by a single cut in non-coding regions are unlikely to produce a functional knockout. A high-throughput method to produce deletions of non-coding DNA is needed. We report a high-throughput genomic deletion strategy to screen for functional long non-coding RNAs (lncRNAs) that is based on a lentiviral paired-guide RNA (pgRNA) library. Applying our screening method, we identified 51 lncRNAs that can positively or negatively regulate human cancer cell growth. We validated 9 of 51 lncRNA hits using CRISPR–Cas9-mediated genomic deletion, functional rescue, CRISPR activation or inhibition and gene-expression profiling. Our high-throughput pgRNA genome deletion method will enable rapid identification of functional mammalian non-coding elements.
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Cited by
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
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CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells
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Towards a complete map of the human long non-coding RNA transcriptome
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Self-citation
Metadata
- Authors
- Zhu, S., Li, W., Liu, J., Chen, C.-H., Liao, Q., Xu, P., Xu, H., Xiao, T., Cao, Z., Peng, J., Yuan, P., Brown, M., Liu, X. S., & Wei, W.
- Venue
- Nature Biotechnology
- Published
- 2016-10-31
- Volume
- 34
- Issue
- 12
- Pages
- 1279-1286
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2544093984
- Cited by
- 425
- References
- 51
- FWCI
- 24.53
- Type
- journal article
- Language
- en
- Open access
- green
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- Zhu, S., Li, W., Liu, J., Chen, C.-H., Liao, Q., Xu, P., Xu, H., Xiao, T., Cao, Z., Peng, J., Yuan, P., Brown, M., Liu, X. S., & Wei, W. (2016). Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR–Cas9 library. Nature Biotechnology. https://doi.org/10.1038/nbt.3715
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