A highly specific SpCas9 variant is identified by in vivo screening in yeast

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CRISPR-Cas9 and beyond: what’s next in plant genome engineering

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Approaches to Enhance Precise CRISPR/Cas9-Mediated Genome Editing

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Engineering synthetic auxotrophs for growth-coupled directed protein evolution

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Guide-specific loss of efficiency and off-target reduction with Cas9 variants

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Innovations in CRISPR technology

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Human Organoids as an Emerging Tool for Genome Screenings

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Isolation of gene-edited cells via knock-in of short glycophosphatidylinositol-anchored epitope tags

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BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors

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Applications of CRISPR Genome Editing to Advance the Next Generation of Adoptive Cell Therapies for Cancer

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An optimized SpCas9 high-fidelity variant for direct protein delivery

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Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNAGln-processing system in human cells

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Massively parallel CRISPRi assays reveal concealed thermodynamic determinants of dCas12a binding

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Engineering of Cas12a nuclease variants with enhanced genome-editing specificity

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Engineered TnpB genome editors for plants and human cells identified by ribonucleoprotein mutational scanning

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Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations

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CoCas9 is a compact nuclease from the human microbiome for efficient and precise genome editing

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Human cellular models of medium spiny neuron development and Huntington disease

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(R)evolution-on-a-chip

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The MyLO CRISPR-Cas9 toolkit: a markerless yeast localization and overexpression CRISPR-Cas9 toolkit

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Current landscape of gene‐editing technology in biomedicine: Applications, advantages, challenges, and perspectives

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Unraveling the future of genomics: CRISPR, single-cell omics, and the applications in cancer and immunology

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The Improvement of CRISPR-Cas9 System With Ubiquitin-Associated Domain Fusion for Efficient Plant Genome Editing

Zheng, X., Qi, C., Yang, L., Quan, Q., Liu, B., Zhong, Z., Tang, X. D., Fan, T., Zhou, J., & Zhang, Y. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00621 · Cited by 22

Generation of mutant pigs by lipofection-mediated genome editing in embryos

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Massively parallel evaluation and computational prediction of the activities and specificities of 17 small Cas9s

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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife

Meaker, G. A., Hair, E. J., & Gorochowski, T. E. (2020) · Synthetic Biology · doi:10.1093/synbio/ysaa021 · Cited by 22

Synthetic Virus-Derived Nanosystems (SVNs) for Delivery and Precision Docking of Large Multifunctional DNA Circuitry in Mammalian Cells

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High-fidelity KKH variant of Staphylococcus aureus Cas9 nucleases with improved base mismatch discrimination

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Understanding the Potential of Genome Editing in Parkinson’s Disease

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Genetic Databases and Gene Editing Tools for Enhancing Crop Resistance against Abiotic Stress

Joshi, A. B., Yang, S.-Y., Song, H.-G., Min, J., & Lee, J.-H. (2023) · Biology · doi:10.3390/biology12111400 · Cited by 21

Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces

Wang, J., Wang, K., Deng, Z., Zhong, Z., Sun, G., Mei, Q., Zhou, F., Deng, Z., & Sun, Y. (2024) · Nature Communications · doi:10.1038/s41467-024-49987-3 · Cited by 20

The application of CRISPR/Cas9–based genome-wide screening to disease research

Chen, X., Zheng, M., Lin, S., Huang, M., Chen, S., & Chen, S. (2024) · Molecular and Cellular Probes · doi:10.1016/j.mcp.2024.102004 · Cited by 21

Cas9HF1 enhanced specificity in Ustilago maydis

Zuo, W., Depotter, J. R. L., & Doehlemann, G. (2020) · Fungal Biology · doi:10.1016/j.funbio.2020.02.006 · Cited by 20

Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1) to nick the target DNA strand

Fan, R., Chai, Z., Xing, S., Chen, K., Qiu, F., Chai, T., Qiu, J., Zhang, Z., Zhang, H., & Gao, C. (2020) · Science China Life Sciences · doi:10.1007/s11427-020-1722-0 · Cited by 20

Resection and repair of a Cas9 double-strand break at CTG trinucleotide repeats induces local and extensive chromosomal deletions

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Characterization of Brevibacillus laterosporus Cas9 (BlatCas9) for Mammalian Genome Editing

Gao, N., Zhang, C., Hu, Z., Li, M., Wei, J., Wang, Y., & Liu, H. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.583164 · Cited by 22

Advancements in CRISPR‐Mediated Multiplex Genome Editing: Transforming Plant Breeding for Crop Improvement and Polygenic Trait Engineering

Kumar, U., Dwivedi, D., & Das, U. (2025) · Biotechnology Journal · doi:10.1002/biot.70148 · Cited by 21

Site-specific genome editing in treatment of inherited diseases: possibility, progress, and perspectives

Huang, C., Li, Q., & Li, J. (2022) · Medical Review · doi:10.1515/mr-2022-0029 · Cited by 20

Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants

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Advancing CRISPR/Cas gene editing with machine learning

Fong, J. H. C., & Wong, A. S. L. (2023) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2023.100477 · Cited by 21

Blossom of CRISPR technologies and applications in disease treatment

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CRISPR genome engineering for retinal diseases

Kantor, A., McClements, M. E., Peddle, C. F., Fry, L. E., Salman, A., Cehajic‐Kapetanovic, J., Xue, K., & MacLaren, R. E. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.024 · Cited by 19

In vivo liver targeted genome editing as therapeutic approach: progresses and challenges

Simoni, C., Barbon, E., Muro, A. F., & Cantore, A. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2024.1458037 · Cited by 20

Methods for the directed evolution of biomolecular interactions

Xie, V. C., Styles, M. J., & Dickinson, B. C. (2022) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2022.01.001 · Cited by 19

The application of CRISPR /Cas mediated gene editing in synthetic biology: Challenges and optimizations

Li, W., Huang, C., & Chen, J. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.890155 · Cited by 22

Utilizing directed evolution to interrogate and optimize CRISPR/Cas guide RNA scaffolds

Bush, K. B., Corsi, G. I., Yan, A. C., Haynes, K., Layzer, J. M., Zhou, J. H., Llanga, T., Gorodkin, J., & Sullenger, B. A. (2023) · Cell chemical biology · doi:10.1016/j.chembiol.2023.06.007 · Cited by 18

CRISPR/Cas9 as a Mutagenic Factor

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Next-generation CRISPR technology for genome, epigenome and mitochondrial editing

Lau, C., Liang, Q., & Zhu, H. (2024) · Transgenic Research · doi:10.1007/s11248-024-00404-x · Cited by 19

The Expanding Class 2 CRISPR Toolbox: Diversity, Applicability, and Targeting Drawbacks

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A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo

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CRISPR/Cas in Grapevine Genome Editing: The Best Is Yet to Come

Ren, C., Mohamed, M. S. M., Aini, N., Kuang, Y., & Liang, Z. (2024) · Horticulturae · doi:10.3390/horticulturae10090965 · Cited by 18

Engineered PsCas9 enables therapeutic genome editing in mouse liver with lipid nanoparticles

Degtev, D., Bravo, J. P. K., Emmanouilidi, A., Zdravković, A., Choong, O. K., Touza, J. L., Selfjord, N., Weisheit, I., Francescatto, M., Akçakaya, P., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-53418-8 · Cited by 19

Innovative Therapies for Cystic Fibrosis: The Road from Treatment to Cure

Cabrini, G. (2018) · Molecular Diagnosis & Therapy · doi:10.1007/s40291-018-0372-6 · Cited by 17

A pipeline for characterization of novel Cas9 orthologs

Karvelis, T., Young, J. K., & Šikšnys, V. (2018) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/bs.mie.2018.10.021 · Cited by 17

Genetically Engineering the Nervous System with CRISPR-Cas

Sandoval, A., Elahi, H., & Ploski, J. E. (2020) · eNeuro · doi:10.1523/eneuro.0419-19.2020 · Cited by 18

Perfecting Targeting in CRISPR

Zhang, H., Li, T., Sun, Y., & Yang, H. (2021) · Annual Review of Genetics · doi:10.1146/annurev-genet-071719-030438 · Cited by 18

Quantitative assessment of engineered Cas9 variants for target specificity enhancement by single-molecule reaction pathway analysis

Bak, S. Y., Jung, Y., Park, J., Sung, K., Jang, H., Bae, S., & Kim, S. K. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab858 · Cited by 17

Decrypting the mechanistic basis of CRISPR/Cas9 protein

Panda, G., & Ray, A. (2022) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2022.05.001 · Cited by 17

Updates and Applications of CRISPR/Cas Technology in Plants

Vidya, N., & Arun, M. (2023) · Journal of Plant Biology · doi:10.1007/s12374-023-09383-8 · Cited by 18

Correcting inborn errors of immunity: From viral mediated gene addition to gene editing

Castiello, M. C., Ferrari, S., & VILLA, A. M. (2023) · Seminars in Immunology · doi:10.1016/j.smim.2023.101731 · Cited by 16

Overcoming the Limitations of CRISPR-Cas9 Systems in Saccharomyces cerevisiae: Off-Target Effects, Epigenome, and Mitochondrial Editing

Sato, G. E., & Kuroda, K. (2023) · Microorganisms · doi:10.3390/microorganisms11041040 · Cited by 16

Precision in Action: The Role of Clustered Regularly Interspaced Short Palindromic Repeats/Cas in Gene Therapies

Banda, A., Impomeni, O., Singh, A., Baloch, A. R., Hu, W., & Jaijyan, D. K. (2024) · Vaccines · doi:10.3390/vaccines12060636 · Cited by 16

SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity

Cerchione, D. M., Loveluck, K., Tillotson, E. L., Harbinski, F., DaSilva, J., Kelley, C. P., Keston-Smith, E., Fernández, C. A., Myer, V. E., Jayaram, H., et al. (2020) · PLoS ONE · doi:10.1371/journal.pone.0231716 · Cited by 16

Rewriting CFTR to cure cystic fibrosis

Maule, G., Ensinck, M. M., Bulcaen, M., & Carlon, M. S. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2020.12.018 · Cited by 17

Genome editing for primary immunodeficiencies: A therapeutic perspective on Wiskott-Aldrich syndrome

Naseem, A., Steinberg, Z., & Cavazza, A. (2022) · Frontiers in Immunology · doi:10.3389/fimmu.2022.966084 · Cited by 16

Improving the on-target activity of high-fidelity Cas9 editors by combining rational design and random mutagenesis

Spasskaya, D. S., Davletshin, A. I., Bachurin, S. S., Tutyaeva, V. V., Garbuz, D. G., & Karpov, D. S. (2023) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-023-12469-5 · Cited by 16

Generation of genetically modified mice using SpCas9-NG engineered nuclease

Fujii, W., Ito, H., Kanke, T., Ikeda, A., Sugiura, K., & Naito, K. (2019) · Scientific Reports · doi:10.1038/s41598-019-49394-5 · Cited by 16

Enhancing cell‐based therapies with synthetic gene circuits responsive to molecular stimuli

Galvan, S., Teixeira, A. P., & Fussenegger, M. (2024) · Biotechnology and Bioengineering · doi:10.1002/bit.28770 · Cited by 16

Evolution-aided engineering of plant specialized metabolism

Irfan, M., Chavez, B. G., Rizzo, P., D’Auria, J. C., & Moghe, G. D. (2021) · aBIOTECH · doi:10.1007/s42994-021-00052-3 · Cited by 15

Recent Updates on Corticosteroid-Induced Neuropsychiatric Disorders and Theranostic Advancements through Gene Editing Tools

Singh, M., Agarwal, V., Jindal, D., Pancham, P., Agarwal, S., Mani, S., Tiwari, R. K., Das, K., Alghamdi, B. S. A., Abujamel, T. S., et al. (2023) · Diagnostics · doi:10.3390/diagnostics13030337 · Cited by 15

Genome editing with natural and engineered CjCas9 orthologs

Gao, S., Wang, Y., Qi, T., Wei, J., Hu, Z., Liu, J., Sun, S., Liu, H., & Wang, Y. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2023.01.029 · Cited by 16

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

Lu, C., Pang, D., Li, M., Yuan, H., Yu, T., Huang, P., Li, J., Chen, X., Jiao, H., Xie, Z., et al. (2019) · Cells · doi:10.3390/cells8020113 · Cited by 15

AAV-mediated FOXG1 gene editing in human Rett primary cells

Croci, S., Carriero, M. L., Capitani, K., Daga, S., Donati, F., Papa, F. T., Frullanti, E., Lopergolo, D., Lamacchia, V., Tita, R., et al. (2020) · European Journal of Human Genetics · doi:10.1038/s41431-020-0652-6 · Cited by 15

Eukaryotic-driven directed evolution of Cas9 nucleases

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Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9

Okada, K., Aoki, K., Tabei, T., Sugio, K., Imai, K., Bonkohara, Y., & Kamachi, Y. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac100 · Cited by 14

Structural basis of Cas9 DNA interrogation with a 5′ truncated sgRNA

Kiernan, K. A., Kwon, J., Merrill, B. J., & Simonović, M. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae1164 · Cited by 13

Gene Editing Therapies Targeting Lipid Metabolism for Cardiovascular Disease: Tools, Delivery Strategies, and Clinical Progress

Ren, Z., Zhou, J., Yang, D., Guo, Y., Zhang, J., Xu, J., & Chen, Y. E. (2026) · Cells · doi:10.3390/cells15020134 · Cited by 13

Non-Viral CRISPR carriers: transient delivery with lasting effects

Lummerstorfer, M., & Lächelt, U. (2026) · Drug Delivery · doi:10.1080/10717544.2026.2614125 · Cited by 14

CRISPR/Cas9: an overview of recent developments and applications in cancer research

Devi, S. N., Rana, R., Malik, P., & Ganguly, N. K. (2024) · International Journal of Surgery · doi:10.1097/js9.0000000000001081 · Cited by 14

Progress in Research and Prospects for Application of Precision Gene-Editing Technology Based on CRISPR–Cas9 in the Genetic Improvement of Sheep and Goats

Lu, Z., Zhang, L., Mu, Q., Liu, J., Chen, Y., Wang, H., Zhang, Y., Su, R., Wang, R., Wang, Z., et al. (2024) · Agriculture · doi:10.3390/agriculture14030487 · Cited by 13

Editing microbes to mitigate enteric methane emissions in livestock

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Light-directed evolution of dynamic, multi-state, and computational protein functionalities

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