Amplification of the Zfy and Zfx Genes for Sex Identification in Humans, Cattle, Sheep and Goats

Aasen, Eric; Medrano, Juan Fernando

doi:10.1038/nbt1290-1279

Cited by 323Genetic and Clinical Aspects of Sex Determination and Chromosomal AbnormalitiesPrenatal Screening and DiagnosticsAnimal Genetics and Reproduction

Amplification of the Zfy and Zfx Genes for Sex Identification in Humans, Cattle, Sheep and Goats

Aasen, Eric, Medrano, Juan Fernando·1990·Nature Biotechnology

DOI 10.1038/nbt1290-1279

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Abstract

We describe a quick and efficient method of determining the sex of DNA samples from humans, cattle, sheep, and goats. Using universal primers we have amplified 447 or 445 bp fragments from male or female genomic DNA corresponding to the ZFY or ZFX genes. Restriction fragment length polymorphism (RFLP) analysis of the fragments yielded specific banding patterns between the two sexes in these species. Horse, pig and rainbow trout fragments did not show RFLPs with the group of enzymes chosen for the other species. Use of this method would allow animal breeders to determine the sex of preimplantation embryos before transfer to recipient females. Human embryos at risk for sex-linked disorders could be sexed soon after in vitro fertilization (IVF) and options considered before implantation.

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The male sex of the muskox specimen was denoted by the presence in the assembly of the X and Y chromosome-specific genes ZFX and ZFY, and the Y chromosome-specific gene SRY.
“The male sex of our muskox specimen was denoted by the presence in our assembly of the X and Y chromosome-specific genes, ZFX (Acc KAL1286804) and ZFY (Acc KAL1286379)39, and the Y chromosome-specific gene, SRY (Acc KAL1286397).”
From Chromosomal-level reference genome assembly of muskox (Ovibos moschatus) from Banks Island in the Canadian Arctic, a resource for conservation genomics (2024) · cites this paper as [39]

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Authors: Aasen, E., & Medrano, J. F.
Affiliations: University of California, Davis (US)
ORCID: Medrano, Juan Fernando
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 1990-12
Volume: 8
Issue: 12
Pages: 1279-1281
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt1290-1279
OpenAlex: W2022126715
Cited by: 323
References: 21
Citations by year: 2026: 5 · 2025: 6 · 2024: 7 · 2023: 8 · 2022: 6 · 2021: 11 · 2020: 12 · 2019: 9 · 2018: 7 · 2017: 10 · 2016: 13 · 2015: 6 · 2014: 8 · 2013: 11 · 2012: 14
FWCI: 3.03
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (unspecified)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
Biotechnology(Faisalabad)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Prenatal Screening and Diagnostics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
Keywords: sex determination, ZFY gene, sex-linked genes, restriction fragment length polymorphism, in vitro fertilization, sex-linked disorders, cattle, sheep, goats, human, universal primers
MeSH terms: Animals, Base Sequence, Cattle, DNA, DNA-Binding Proteins, Female, Goats, Humans, Male, Molecular Sequence Data, Oligonucleotides, Polymorphism, Restriction Fragment Length, Sex Determination Analysis, Sheep, Transcription Factors, Polymerase Chain Reaction, Kruppel-Like Transcription Factors

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Amplification of the Zfy and Zfx Genes for Sex Identification in Humans, Cattle, Sheep and Goats

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