The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol
Abstract
We identify a cytochrome P450 gene (CYP6AE14) from cotton bollworm (Helicoverpa armigera), which permits this herbivore to tolerate otherwise inhibitory concentrations of the cotton metabolite, gossypol. CYP6AE14 is highly expressed in the midgut and its expression correlates with larval growth when gossypol is included in the diet. When larvae are fed plant material expressing double-stranded RNA (dsRNA) specific to CYP6AE14, levels of this transcript in the midgut decrease and larval growth is retarded. Both effects are more dramatic in the presence of gossypol. As a glutathione-S-transferase gene (GST1) is silenced in GST1 dsRNA-expressing plants, feeding insects plant material expressing dsRNA may be a general strategy to trigger RNA interference and could find applications in entomological research and field control of insect pests.
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“The potential of RNAi for pest control has first been demonstrated in 2007 for H. armigera and Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) (Baum et al., 2007;Mao et al., 2007).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Mao et al., 2007
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Cites
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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
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Specific interference by ingested dsRNA
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Construct design for efficient, effective and high‐throughput gene silencing in plants
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The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
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Superoxide radical inhibits catalase.
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Mechanisms of plant defense against insect herbivores
War, A. R., Paulraj, M. G., Ahmad, T., Buhroo, A. A., Hussain, B., Savarimuthu Ignacimuthu, S. J., & Sharma, H. C. (2012) · Plant Signaling & Behavior · doi:10.4161/psb.21663 · Cited by 2,214
Perspectives for nano-biotechnology enabled protection and nutrition of plants
Ghormade, V., Deshpande, M. V., & Paknikar, K. M. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.06.007 · Cited by 1,200
“…knockdown was reported in several insect orders, including Diptera, Coleoptera, Hymenoptera, Orthoptera, Blattodea, Lepidoptera and Hemiptera (Araujo et al., 2006; Baum et al., 2007; Huvenne and Smagghe, 2010;Mao et al., 2007; Timmons and Fire, 1998; Tomoyasu and Denell, 2004; Turner et al., 2006).”
InfluentialInsect resistance to Bt crops: lessons from the first billion acres
Tabashnik, B. E., Brévault, T., & Carrière, Y. (2013) · Nature Biotechnology · doi:10.1038/nbt.2597 · Cited by 1,086
Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: A review
Huvenne, H., & Smagghe, G. J. (2009) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2009.10.004 · Cited by 996
New Insights into Plant Responses to the Attack from Insect Herbivores
Wu, J., & Baldwin, I. T. (2010) · Annual Review of Genetics · doi:10.1146/annurev-genet-102209-163500 · Cited by 941
“Although the range of applicable herbivore species is unclear, expressing herbivore-targeting RNAi constructs in plants to silence the expression of particular genes in herbivores is an exciting new approach that would revolutionize the study of the coevolutionary dynamics of both partners in the plant-herbivore inter…”
Background
Metadata
- Authors
- Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Chen, Xiao-Ya
- Venue
- Nature Biotechnology
- Published
- 2007-11
- Volume
- 25
- Issue
- 11
- Pages
- 1307-1313
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2121440246
- Cited by
- 1,358
- References
- 50
- Citations by year
- 2026: 44 · 2025: 76 · 2024: 80 · 2023: 56 · 2022: 111 · 2021: 98 · 2020: 84 · 2019: 87 · 2018: 67 · 2017: 95 · 2016: 92 · 2015: 89 · 2014: 84 · 2013: 77 · 2012: 68
- FWCI
- 13.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Keywords
- Helicoverpa armigera, cytochrome P450 monooxygenase, dsRNA feeding, midgut gene expression, larval growth, insect pest control, plant-insect interactions
- MeSH terms
- Animals, Gossypium, Cytochrome P-450 Enzyme System, Digestion, Drug Resistance, Glutathione Transferase, Gossypol, Larva, Lepidoptera, Molecular Sequence Data, Pest Control, Biological, RNA, Double-Stranded, Nicotiana, Arabidopsis, RNA, Plant, Insect Proteins, Gene Silencing, Plants, Genetically Modified, RNA Interference, RNA, Small Interfering, Cytochrome P-450 Enzyme Inhibitors
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