The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol
Abstract
We identify a cytochrome P450 gene (CYP6AE14) from cotton bollworm (Helicoverpa armigera), which permits this herbivore to tolerate otherwise inhibitory concentrations of the cotton metabolite, gossypol. CYP6AE14 is highly expressed in the midgut and its expression correlates with larval growth when gossypol is included in the diet. When larvae are fed plant material expressing double-stranded RNA (dsRNA) specific to CYP6AE14, levels of this transcript in the midgut decrease and larval growth is retarded. Both effects are more dramatic in the presence of gossypol. As a glutathione-S-transferase gene (GST1) is silenced in GST1 dsRNA-expressing plants, feeding insects plant material expressing dsRNA may be a general strategy to trigger RNA interference and could find applications in entomological research and field control of insect pests.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The potential of RNAi for pest control has first been demonstrated in 2007 for H. armigera and Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) (Baum et al., 2007;Mao et al., 2007).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Mao et al., 2007
Citations
Cites
Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Specific interference by ingested dsRNA
Timmons, L., & Fire, A. Z. (1998) · Nature · doi:10.1038/27579 · Cited by 1,938
Timmons, L., Court, D. L., & Fire, A. Z. (2001) · Gene · doi:10.1016/s0378-1119(00)00579-5 · Cited by 1,840
Genetic and Functional Diversification of Small RNA Pathways in Plants
Xie, Z., Johansen, L. K., Gustafson, A. M., Kasschau, K. D., Lellis, A. D., Zilberman, D., Jacobsen, S. E., & Carrington, J. C. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020104 · Cited by 1,569
Construct design for efficient, effective and high‐throughput gene silencing in plants
Wesley, S. V., Helliwell, C. A., Smith, N. A., Wang, M., Rouse, D. T., Liu, Q., Gooding, P. S., Singh, S. P., Abbott, D., Stoutjesdijk, P. A., et al. (2001) · The Plant Journal · doi:10.1046/j.1365-313x.2001.01105.x · Cited by 1,542
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
Tabara, H., Sarkissian, M., Kelly, W. G., Fleenor, J., Grishok, A., Timmons, L., Fire, A. Z., & Mello, C. C. (1999) · Cell · doi:10.1016/s0092-8674(00)81644-x · Cited by 1,307
Superoxide radical inhibits catalase.
Kono, Y., & Fridovich, I. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83842-5 · Cited by 1,254
Systemic RNAi in C. elegans Requires the Putative Transmembrane Protein SID-1
Winston, W. M., Molodowitch, C., & Hunter, C. P. (2002) · Science · doi:10.1126/science.1068836 · Cited by 849
Aravin, A. A., Nm, N., Tulin, A. V., Vagin, V. V., Rozovsky, Y. M., & Gvozdev, V. A. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00299-8 · Cited by 827
Economic, Ecological, Food Safety, and Social Consequences of the Deployment of Bt Transgenic Plants
Shelton, A. M., Zhao, J.-Z., & Roush, R. T. (2002) · Annual Review of Entomology · doi:10.1146/annurev.ento.47.091201.145309 · Cited by 794
Palauqui, J., Elmayan, T., Pollien, J., & Vaucheret, H. (1997) · The EMBO Journal · doi:10.1093/emboj/16.15.4738 · Cited by 780
Plant resistance towards insect herbivores: a dynamic interaction
Gatehouse, J. A. (2002) · New Phytologist · doi:10.1046/j.1469-8137.2002.00519.x · Cited by 704
RNAi in C. elegans : Soaking in the Genome Sequence
Tabara, H., Grishok, A., & Mello, C. C. (1998) · Science · doi:10.1126/science.282.5388.430 · Cited by 668
Huang, G., Allen, R., Davis, E., Baum, T. J., & HUSSEY, R. S. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604698103 · Cited by 633
Elucidation of the Small RNA Component of the Transcriptome
Lu, C., Tej, S. S., Luo, S. J., Haudenschild, C. D., Meyers, B. C., & Green, P. J. (2005) · Science · doi:10.1126/science.1114112 · Cited by 632
Systemic signalling in gene silencing
Voinnet, O., & Baulcombe, D. C. (1997) · Nature · doi:10.1038/39215 · Cited by 628
Identification of a Gene Associated with Bt Resistance in Heliothis virescens
Gahan, L. J., Gould, F. L., & Heckel, D. G. (2001) · Science · doi:10.1126/science.1060949 · Cited by 622
Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance
Niu, Q., Lin, S., Reyes, J. L., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1255 · Cited by 565
Bouché, N., Lauressergues, D., Gasciolli, V., et al. (2006) · The EMBO Journal · doi:10.1038/sj.emboj.7601217 · Cited by 516
Johansen, L. K., & Carrington, J. C. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.126.3.930 · Cited by 513
Successful herbivore attack due to metabolic diversion of a plant chemical defense
Wittstock, U., Agerbirk, N., Stauber, E. J., Olsen, C. E., Hippler, M., Mitchell‐Olds, T., Gershenzon, J., & Vogel, H. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0308007101 · Cited by 509
Turner, C. T., Davy, M. W., MacDiarmid, R. M., Plummer, K. M., Birch, N. P., & Newcomb, R. D. (2006) · Insect Molecular Biology · doi:10.1111/j.1365-2583.2006.00656.x · Cited by 346
Long-term regional suppression of pink bollworm by Bacillus thuringiensis cotton
Carrière, Y., Ellers-Kirk, C., Sisterson, M., Antilla, L., Whitlow, M., Dennehy, T. J., & Tabashnik, B. E. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0436708100 · Cited by 320
Cytochromes P450 of insects: the tip of the iceberg
Scott, J. G., & Wen, Z. (2001) · Pest Management Science · doi:10.1002/ps.354 · Cited by 318
Smith, L. M., Pontes, O., Searle, I. R., et al. (2007) · The Plant Cell · doi:10.1105/tpc.107.051540 · Cited by 286
Jasmonate and salicylate induce expression of herbivore cytochrome P450 genes
Li, X., Schuler, M. A., & Berenbaum, M. R. (2002) · Nature · doi:10.1038/nature01003 · Cited by 265
Double-Stranded RNA-Mediated Interference with Plant Virus Infection
Tenllado, F., & Díaz-Ruíz, J. R. (2001) · Journal of Virology · doi:10.1128/jvi.75.24.12288-12297.2001 · Cited by 249
Insect cytochromes P450: diversity, insecticide resistance and tolerance to plant toxins
Scott, J. G., Liu, N., & Wen, Z. (1998) · Comparative Biochemistry and Physiology Part C Pharmacology Toxicology and Endocrinology · doi:10.1016/s0742-8413(98)10035-x · Cited by 244
Cytochrome P450 monooxygenases and insecticide resistance in insects
Bergé, J., Feyereisen, R., & Amichot, M. (1998) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.1998.0321 · Cited by 229
Luo, P., Wang, Y., Wang, G., Essenberg, M., & Chen, X. (2001) · The Plant Journal · doi:10.1046/j.1365-313x.2001.01133.x · Cited by 160
Hemolin gene silencing by ds‐RNA injected into Cecropia pupae is lethal to next generation embryos
Bettencourt, R., Terenius, O., & Faye, I. (2002) · Insect Molecular Biology · doi:10.1046/j.1365-2583.2002.00334.x · Cited by 158
Sasabe, M., Wen, Z., Berenbaum, M. R., et al. (2004) · Gene · doi:10.1016/j.gene.2004.04.028 · Cited by 145
Du, L., Ge, F., Zhu, S., & Parajulee, M. N. (2004) · Journal of Economic Entomology · doi:10.1093/jee/97.4.1278 · Cited by 130
Eleftherianos, I., Marokházi, J., Millichap, P. J., et al. (2006) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2006.04.001 · Cited by 124
Waters, L. C., Zelhof, A. C., Shaw, B. J., et al. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.11.4855 · Cited by 122
Stipanovic, R. D., López, J. D., Dowd, M. K., Puckhaber, L. S., & Duke, S. E. (2006) · Journal of Chemical Ecology · doi:10.1007/s10886-006-9052-9 · Cited by 119
Targeted disruption of genes in the Bombyx mori sex pheromone biosynthetic pathway
Ohnishi, A., Hull, J. J., & Matsumoto, S. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0511270103 · Cited by 119
Plant allelochemicals differentially regulate Helicoverpa zea cytochrome P450 genes
Li, X., Berenbaum, M. R., & Schuler, M. A. (2002) · Insect Molecular Biology · doi:10.1046/j.1365-2583.2002.00341.x · Cited by 117
Mazumdar–Leighton, S., & Broadway, R. M. (2001) · Insect Biochemistry and Molecular Biology · doi:10.1016/s0965-1748(00)00168-5 · Cited by 114
Ranasinghe, C., & Hobbs, A. A. (1998) · Insect Biochemistry and Molecular Biology · doi:10.1016/s0965-1748(98)00045-9 · Cited by 91
Xiaoping, W., & Hobbs, A. A. (1995) · Insect Biochemistry and Molecular Biology · doi:10.1016/0965-1748(95)00033-r · Cited by 82
Tan, X., Liang, W., Liu, C.-J., et al. (2000) · Planta · doi:10.1007/s004250050055 · Cited by 64
Pesticide-metabolizing enzymes
Hodgson, E., Rose, R. L., Ryu, D., Falls, G., Blake, B. L., & Levi, P. E. (1995) · Toxicology Letters · doi:10.1016/0378-4274(95)03469-2 · Cited by 62
Li, W., Petersen, R. A., Schuler, M. A., et al. (2002) · Insect Molecular Biology · doi:10.1046/j.1365-2583.2002.00363.x · Cited by 61
Wang, S., Young, F., & Hickey, D. A. (1995) · Insect Biochemistry and Molecular Biology · doi:10.1016/0965-1748(95)00022-n · Cited by 56
Benedict, C. R., Martin, G. S., Liu, J., et al. (2004) · Phytochemistry · doi:10.1016/j.phytochem.2004.03.032 · Cited by 49
Li, W., Zangerl, A. R., Schuler, M. A., et al. (2004) · Insect Molecular Biology · doi:10.1111/j.0962-1075.2004.00518.x · Cited by 46
Preliminary studies on differential defense responses induced during plant communication
Jin, P., Li, Z. H., XIANG, H., et al. (2005) · Cell Research · doi:10.1038/sj.cr.7290285 · Cited by 24
Ranasinghe, C., & Hobbs, A. A. (1999) · Insect Biochemistry and Molecular Biology · doi:10.1016/s0965-1748(98)00118-0 · Cited by 15
Flagged first, then by citations
Cited by
Sorted by citations
Mechanisms of plant defense against insect herbivores
War, A. R., Paulraj, M. G., Ahmad, T., Buhroo, A. A., Hussain, B., Savarimuthu Ignacimuthu, S. J., & Sharma, H. C. (2012) · Plant Signaling & Behavior · doi:10.4161/psb.21663 · Cited by 2,214
Perspectives for nano-biotechnology enabled protection and nutrition of plants
Ghormade, V., Deshpande, M. V., & Paknikar, K. M. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.06.007 · Cited by 1,200
“…knockdown was reported in several insect orders, including Diptera, Coleoptera, Hymenoptera, Orthoptera, Blattodea, Lepidoptera and Hemiptera (Araujo et al., 2006; Baum et al., 2007; Huvenne and Smagghe, 2010;Mao et al., 2007; Timmons and Fire, 1998; Tomoyasu and Denell, 2004; Turner et al., 2006).”
InfluentialInsect resistance to Bt crops: lessons from the first billion acres
Tabashnik, B. E., Brévault, T., & Carrière, Y. (2013) · Nature Biotechnology · doi:10.1038/nbt.2597 · Cited by 1,086
Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: A review
Huvenne, H., & Smagghe, G. J. (2009) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2009.10.004 · Cited by 996
New Insights into Plant Responses to the Attack from Insect Herbivores
Wu, J., & Baldwin, I. T. (2010) · Annual Review of Genetics · doi:10.1146/annurev-genet-102209-163500 · Cited by 941
“Although the range of applicable herbivore species is unclear, expressing herbivore-targeting RNAi constructs in plants to silence the expression of particular genes in herbivores is an exciting new approach that would revolutionize the study of the coevolutionary dynamics of both partners in the plant-herbivore inter…”
Background
Metadata
- Authors
- Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Chen, Xiao-Ya
- Venue
- Nature Biotechnology
- Published
- 2007-11
- Volume
- 25
- Issue
- 11
- Pages
- 1307-1313
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2121440246
- Cited by
- 1,358
- References
- 50
- Citations by year
- 2026: 44 · 2025: 76 · 2024: 80 · 2023: 56 · 2022: 111 · 2021: 98 · 2020: 84 · 2019: 87 · 2018: 67 · 2017: 95 · 2016: 92 · 2015: 89 · 2014: 84 · 2013: 77 · 2012: 68
- FWCI
- 13.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Keywords
- Helicoverpa armigera, cytochrome P450 monooxygenase, dsRNA feeding, midgut gene expression, larval growth, insect pest control, plant-insect interactions
- MeSH terms
- Animals, Gossypium, Cytochrome P-450 Enzyme System, Digestion, Drug Resistance, Glutathione Transferase, Gossypol, Larva, Lepidoptera, Molecular Sequence Data, Pest Control, Biological, RNA, Double-Stranded, Nicotiana, Arabidopsis, RNA, Plant, Insect Proteins, Gene Silencing, Plants, Genetically Modified, RNA Interference, RNA, Small Interfering, Cytochrome P-450 Enzyme Inhibitors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review