CoChat
Sign Up
Cited by 224

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

PtdIns(3,4,5)P3 binding is necessary for WAVE2-induced formation of lamellipodia

Oikawa, Tsukasa, Yamaguchi, Hideki, Itoh, Toshiki, Kato, Masayoshi, Ijuin, Takeshi, Yamazaki, Daisuke, Suetsugu, Shiro, Takenawa, Tadaomi·2004·Nature Cell Biology
DOI 10.1038/ncb1125
Open this paper in CoChat — free account

Abstract

Polarized cell movement is triggered by the development of a PtdIns(3,4,5)P(3) gradient at the membrane, which is followed by rearrangement of the actin cytoskeleton. The WASP family verprolin homologous protein (WAVE) is essential for lamellipodium formation at the leading edge by activating the Arp2/3 complex downstream of Rac GTPase. Here, we report that WAVE2 binds to PtdIns(3,4,5)P(3) through its basic domain. The amino-terminal portion of WAVE2, which includes the PtdIns(3,4,5)P(3)-binding sequence, was localized at the leading edge of lamellipodia induced by an active form of Rac (RacDA) or by treatment with platelet-derived growth factor (PDGF). Production of PtdIns(3,4,5)P(3) at the cell membrane by myristoylated phosphatidylinositol-3-OH kinase (PI(3)K) is sufficient to recruit WAVE2 in the presence of dominant-negative Rac and latrunculin, demonstrating that PtdIns(3,4,5)P(3) alone is able to recruit WAVE2. Expression of a full-length mutant of WAVE2 that lacks the lipid-binding activity inhibited proper formation of lamellipodia induced by RacDA. These results suggest that one of the products of PI(3)K, PtdIns(3,4,5)P(3), recruits WAVE2 to the polarized membrane and that this recruitment is essential for lamellipodium formation at the leading edge.

Overview

Integrity signals

Registry

Crossref · 10.1038/ncb1125

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 29

  1. Rickert, P., Weiner, O.D., Wang, F., Bourne, H.R. & Servant, G. Leukocytes navigate by compass: roles of PI(3)Kgamma and its lipid products. Trends Cell Biol. 10, 466–473 (2000). · DOI
  2. Insall, R.H. & Weiner, O.D. PIP3, PIP2, and cell movement — similar messages, different meanings? Dev. Cell 1, 743–747 (2001). · DOI
  3. Chung, C.Y., Funamoto, S. & Firtel, R.A. Signaling pathways controlling cell polarity and chemotaxis. Trends Biochem. Sci. 26, 557–566 (2001). · DOI
  4. Pollard, T.D. & Borisy, G.G. Cellular motility driven by assembly and disassembly of actin filaments. Cell 112, 453–465 (2003). · DOI
  5. Nobes, C.D. & Hall, A. Rho GTPases control polarity, protrusion, and adhesion during cell movement. J. Cell Biol. 144, 1235–1244 (1999). · DOI
  6. Ridley, A.J. Rho GTPases and cell migration. J. Cell Sci. 114, 2713–2722 (2001). · DOI
  7. Hall, A. Rho GTPases and the actin cytoskeleton. Science 279, 509–514 (1998). · DOI
  8. Prehoda, K.E., Scott, J.A., Mullins, R.D. & Lim, W.A. Integration of multiple signals through cooperative regulation of the N-WASP-Arp2/3 complex. Science 290, 801–806 (2000). · DOI
  9. Rohatgi, R., Ho, H.Y. & Kirschner, M.W. Mechanism of N-WASP activation by CDC42 and phosphatidylinositol 4, 5-bisphosphate. J. Cell Biol. 150, 1299–1310 (2000). · DOI
  10. Miki, H., Suetsugu, S. & Takenawa, T. WAVE, a novel WASP-family protein involved in actin reorganization induced by Rac. EMBO J. 17, 6932–6941 (1998). · DOI
  11. Miki, H., Yamaguchi, H., Suetsugu, S. & Takenawa, T. IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling. Nature 408, 732–735 (2000). · DOI
  12. Suetsugu, S., Miki, H. & Takenawa, T. Identification of two human WAVE/SCAR homologues as general actin regulatory molecules which associate with the Arp2/3 complex. Biochem. Biophys. Res. Commun. 260, 296–302 (1999). · DOI · Paper page
  13. Yamazaki, D. et al. WAVE2 is required for directed cell migration and cardiovascular development. Nature 424, 452–456 (2003). · DOI
  14. Yan, C. et al. WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motility. EMBO J. 22, 3602–3612 (2003). · DOI · Paper page
  15. Suetsugu, S., Yamazaki, D., Kurisu, S. & Takenawa, T. Differential roles of WAVE1 and WAVE2 in dorsal and peripheral ruffle formation for fibroblast cell migration. Dev. Cell 5, 595–609 (2003). · DOI
  16. Takenawa, T. & Itoh, T. Phosphoinositides, key molecules for regulation of actin cytoskeletal organization and membrane traffic from the plasma membrane. Biochim. Biophys. Acta 1533, 190–206 (2001). · DOI
  17. Cross, G.H. et al. A new quantitative optical biosensor for protein characterisation. Biosens. Bioelectron. 19, 383–390 (2003). · DOI
  18. Benard, V., Bohl, B.P. & Bokoch, G.M. Characterization of rac and cdc42 activation in chemoattractant-stimulated human neutrophils using a novel assay for active GTPases. J. Biol. Chem. 274, 13198–13204 (1999). · DOI
  19. Weiner, O.D. Regulation of cell polarity during eukaryotic chemotaxis: the chemotactic compass. Curr. Opin. Cell Biol. 14, 196–202 (2002). · DOI
  20. Wang, F. et al. Lipid products of PI(3)Ks maintain persistent cell polarity and directed motility in neutrophils. Nature Cell Biol. 4, 513–518 (2002). · DOI
  21. Weiner, O.D. et al. A PtdInsP(3)- and Rho GTPase-mediated positive feedback loop regulates neutrophil polarity. Nature Cell Biol. 4, 509–513 (2002). · DOI
  22. Kitamura, T. et al. Insulin-induced phosphorylation and activation of cyclic nucleotide phosphodiesterase 3B by the serine-threonine kinase Akt. Mol. Cell Biol. 19, 6286–6296 (1999). · DOI
  23. Tengholm, A. & Meyer, T. A PI3-kinase signaling code for insulin-triggered insertion of glucose transporters into the plasma membrane. Curr. Biol. 12, 1871–1876 (2002). · DOI
  24. Srinivasan, S. et al. Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis. J. Cell Biol. 160, 375–385 (2003). · DOI
  25. Servant, G. et al. Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science 287, 1037–1040 (2000). · DOI
  26. Iijima, M. & Devreotes, P. Tumor suppressor PTEN mediates sensing of chemoattractant gradients. Cell 109, 599–610 (2002). · DOI
  27. Funamoto, S., Meili, R., Lee, S., Parry, L. & Firtel, R.A. Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis. Cell 109, 611–623 (2002). · DOI
  28. Eden, S., Rohatgi, R., Podtelejnikov, A.V., Mann, M. & Kirschner, M.W. Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck. Nature 418, 790–793 (2002). · DOI · Paper page
  29. Steffen, A. et al. Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation. EMBO J. 23, 749–759 (2004). · DOI

Cited by

Sorted by citations

Metadata

Authors
Oikawa, T., Yamaguchi, H., Itoh, T., Kato, M., Ijuin, T., Yamazaki, D., Suetsugu, S., & Takenawa, T.
Venue
Nature Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2004-04-25
Volume
6
Issue
5
Pages
420-426
ISSN
  • 1465-7392
  • 1476-4679
DOI
10.1038/ncb1125
Cited by
224
References
1
Language
en
Licence
www.springer.com/tdm (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review