The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
DNA processing is not required for ATM-mediated telomere damage response after TRF2 deletion
Abstract
Telomere attrition and other forms of telomere damage can activate the ATM kinase pathway. What generates the DNA damage signal at mammalian chromosome ends or at other double-strand breaks is not known. Telomere dysfunction is often accompanied by disappearance of the 3' telomeric overhang, raising the possibility that DNA degradation could generate the structure that signals. Here we address these issues by studying telomere structure after conditional deletion of mouse TRF2, the protective factor at telomeres. Upon removal of TRF2 from TRF2(F/-) p53-/- mouse embryo fibroblasts, a telomere damage response is observed at most chromosome ends. As expected, the telomeres lose the 3' overhang and are processed by the non-homologous end-joining pathway. Non-homologous end joining of telomeres was abrogated in DNA ligase IV-deficient (Lig4-/-) cells. Unexpectedly, the telomeres of TRF2-/- Lig4-/- p53-/- cells persisted in a free state without undergoing detectable DNA degradation. Notably, the telomeres retained their 3' overhangs, but they were recognized as sites of DNA damage, accumulating the DNA damage response factors 53BP1 and gamma-H2AX, and activating the ATM kinase. Thus, activation of the ATM kinase pathway at chromosome ends does not require overhang degradation or other overt DNA processing.
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In the citing paper
“By contrast, when telomeres are compromised through the loss of TRF2, their repair is carried out by c-NHEJ80–82.”
From Telomeres in cancer: tumour suppression and genome instability (2017) · cites this paper as [81]
In the citing paper
“Similarly, acute telomere deprotection by inactivation of the shelterin protein TRF2 leads to robust telomeric ATM signaling, NHEJ-dependent telomere end-to-end fusion, and rapid cell cycle arrest [71,95].”
From Mechanisms and Regulation of Cellular Senescence (2021) · cites this paper as [95]
In the citing paper
“TRF2 is critical for telomere end protection and can facilitate formation of the t-loop conformation.”
From Telomeres and telomerase in cancer (2010) · cites this paper as [4]
In the citing paper
“Disruption of TRF2 through overexpression of a dominant-negative form or through deletion in TRF2-knockout mouse embryo fibroblasts leads to loss of the protective capped structure, characterized by processing of the 3# overhang and ligation of chromosome ends (3,4).”
From Telomeres and telomerase in cancer (2010) · cites this paper as [4]
Citations
Cites
A biomarker that identifies senescent human cells in culture and in aging skin in vivo.
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DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation
Bakkenist, C. J., & Kastan, M. B. (2003) · Nature · doi:10.1038/nature01368 · Cited by 3,466
A DNA damage checkpoint response in telomere-initiated senescence
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Sensing DNA Damage Through ATRIP Recognition of RPA-ssDNA Complexes
Zou, L., & Elledge, S. J. (2003) · Science · doi:10.1126/science.1083430 · Cited by 2,693
Mammalian Telomeres End in a Large Duplex Loop
Griffith, J. D., Comeau, L., Rosenfield, S., Stansel, R. M., Bianchi, A., Moss, H., & de Lange, T. (1999) · Cell · doi:10.1016/s0092-8674(00)80760-6 · Cited by 2,447
Tumor spectrum analysis in p53-mutant mice
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TRF2 Protects Human Telomeres from End-to-End Fusions
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DNA Damage Foci at Dysfunctional Telomeres
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Cited by
Sorted by citations
Shelterin: the protein complex that shapes and safeguards human telomeres
de Lange, T. (2005) · Genes & Development · doi:10.1101/gad.1346005 · Cited by 3,056
How Shelterin Protects Mammalian Telomeres
Palm, W., & de Lange, T. (2008) · Annual Review of Genetics · doi:10.1146/annurev.genet.41.110306.130350 · Cited by 1,802
The multifaceted roles of PARP1 in DNA repair and chromatin remodelling
Ray Chaudhuri, A., & Nussenzweig, A. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.53 · Cited by 1,786
Living on a break: cellular senescence as a DNA-damage response
d'Adda di Fagagna, F. (2008) · Nature Reviews Cancer · doi:10.1038/nrc2440 · Cited by 1,170
Telomeres: protecting chromosomes against genome instability
O’Sullivan, R. J., & Karlseder, J. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2848 · Cited by 1,043
Metadata
- Authors
- Celli, G. B., & de Lange, T.
- Affiliations
- NYU Langone Health (US)
- Venue
- Nature Cell Biology
- Published
- 2005-06-19
- Volume
- 7
- Issue
- 7
- Pages
- 712-718
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2035389897
- Semantic Scholar
- f4e3c6cbe3167cb507269beefcc7ad01ad45332d
- Cited by
- 623
- References
- 36
- Citations by year
- 2026: 9 · 2025: 14 · 2024: 28 · 2023: 19 · 2022: 16 · 2021: 27 · 2020: 26 · 2019: 20 · 2018: 22 · 2017: 37 · 2016: 24 · 2015: 37 · 2014: 41 · 2013: 37 · 2012: 41
- FWCI
- 20.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM49046-12)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- ATM kinase, TRF2, telomere shortening, non-homologous end joining, DNA ligase IV, DNA damage signaling, 53BP1, γH2AX, telomere dysfunction, p53-deficient cells
- MeSH terms
- Tumor Suppressor p53-Binding Protein 1, DNA Ligase ATP, Animals, Cells, Cultured, Chromosomal Proteins, Non-Histone, Crosses, Genetic, DNA, DNA Damage, DNA-Binding Proteins, Embryo, Mammalian, Fibroblasts, Histones, Mice, Transgenic, Phosphoproteins, Phosphorylation, DNA Ligases, Transfection, Tumor Suppressor Protein p53, Telomere, Protein Serine-Threonine Kinases, Gene Deletion, In Situ Hybridization, Fluorescence, Mice, Knockout, Cell Cycle Proteins, rap1 GTP-Binding Proteins, Tumor Suppressor Proteins, Telomeric Repeat Binding Protein 1, Telomeric Repeat Binding Protein 2, Intracellular Signaling Peptides and Proteins, Mice, Ataxia Telangiectasia Mutated Proteins, Checkpoint Kinase 2
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