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Cited by 797Cellular Mechanics and InteractionsCell Adhesion Molecules ResearchSkin and Cellular Biology Research

Actin and α-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner

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Choi, Colin K., Vicente-Manzanares, Miguel, Zareno, Jessica, Whitmore, Leanna A., Mogilner, Alex, Horwitz, Alan Rick·2008·Nature Cell Biology
DOI 10.1038/ncb1763PMC PMC2827253
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Abstract

Using two-colour imaging and high resolution TIRF microscopy, we investigated the assembly and maturation of nascent adhesions in migrating cells. We show that nascent adhesions assemble and are stable within the lamellipodium. The assembly is independent of myosin II but its rate is proportional to the protrusion rate and requires actin polymerization. At the lamellipodium back, the nascent adhesions either disassemble or mature through growth and elongation. Maturation occurs along an alpha-actinin-actin template that elongates centripetally from nascent adhesions. Alpha-Actinin mediates the formation of the template and organization of adhesions associated with actin filaments, suggesting that actin crosslinking has a major role in this process. Adhesion maturation also requires myosin II. Rescue of a myosin IIA knockdown with an actin-bound but motor-inhibited mutant of myosin IIA shows that the actin crosslinking function of myosin II mediates initial adhesion maturation. From these studies, we have developed a model for adhesion assembly that clarifies the relative contributions of myosin II and actin polymerization and organization.

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Cited by

Sorted by citations

Authors
Choi, C. K., Vicente-Manzanares, M., Zareno, J., Whitmore, L. A., Mogilner, A., & Horwitz, A. R.
Affiliations
  • University of Virginia (US)
  • University of California, Davis (US)
ORCID
Mogilner, Alex
Venue
Nature Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2008-08-17
Volume
10
Issue
9
Pages
1039-1050
ISSN
  • 1465-7392 (ISSN-L)
  • 1476-4679
DOI
10.1038/ncb1763
PMC
PMC2827253
OpenAlex
W1979038578
Cited by
797
References
1
Citations by year
2026: 20 · 2025: 25 · 2024: 30 · 2023: 33 · 2022: 37 · 2021: 48 · 2020: 35 · 2019: 45 · 2018: 37 · 2017: 55 · 2016: 39 · 2015: 48 · 2014: 66 · 2013: 67 · 2012: 61
FWCI
20.49
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (5u54gm064346-10)
  • National Institute of General Medical Sciences (U54-GM064346, R37GM023244, R01GM023244, GM 23244)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
Keywords
nascent adhesions, alpha-actinin, myosin II, myosin IIA, actin polymerization, actin cross-linking, lamellipodia, cell migration, cell protrusions, actin filament organization
MeSH terms
Actinin, Actins, Animals, Cell Adhesion, Cricetulus, Cross-Linking Reagents, Cricetinae, Humans, Models, Biological, Pseudopodia, CHO Cells, Cell Polarity, Nonmuscle Myosin Type IIA, Rats

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