The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly
Open accessAbstract
Stress granules (SGs) and processing bodies (PBs) are microscopically visible ribonucleoprotein granules that cooperatively regulate the translation and decay of messenger RNA. Using an RNA-mediated interference-based screen, we identify 101 human genes required for SG assembly, 39 genes required for PB assembly, and 31 genes required for coordinate SG and PB assembly. Although 51 genes encode proteins involved in mRNA translation, splicing and transcription, most are not obviously associated with RNA metabolism. We find that several components of the hexosamine biosynthetic pathway, which reversibly modifies proteins with O-linked N-acetylglucosamine (O-GlcNAc) in response to stress, are required for SG and PB assembly. O-GlcNAc-modified proteins are prominent components of SGs but not PBs, and include RACK1 (receptor for activated C kinase 1), prohibitin-2, glyceraldehyde-3-phosphate dehydrogenase and numerous ribosomal proteins. Our results suggest that O-GlcNAc modification of the translational machinery is required for aggregation of untranslated messenger ribonucleoproteins into SGs. The lack of enzymes of the hexosamine biosynthetic pathway in budding yeast may contribute to differences between mammalian SGs and related yeast EGP (eIF4E, 4G and Pab1 containing) bodies.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Stress granules and processing bodies are dynamically linked sites of mRNP remodeling
Kedersha, N., Stoecklin, G., Ayodele, M., Yacono, P., Lykke-Andersen, J., Fritzler, M. J., Scheuner, D., Kaufman, R. J., Golan, D. E., & Anderson, P. (2005) · The Journal of Cell Biology · doi:10.1083/jcb.200502088 · Cited by 1,490
Self-citationDecapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies
Sheth, U., & Parker, R. (2003) · Science · doi:10.1126/science.1082320 · Cited by 1,365
P bodies and the control of mRNA translation and degradation
Parker, R., & Sheth, U. (2007) · Molecular Cell · doi:10.1016/j.molcel.2007.02.011 · Cited by 1,335
Stress granules: the Tao of RNA triage
Anderson, P., & Kedersha, N. (2008) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.12.003 · Cited by 1,116
Self-citationStress Granule Assembly Is Mediated by Prion-like Aggregation of TIA-1
Gilks, N., Kedersha, N., Ayodele, M., Shen, L., Stoecklin, G., Dember, L. M., & Anderson, P. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-08-0715 · Cited by 996
Self-citationAnderson, P., & Kedersha, N. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200512082 · Cited by 984
Self-citationP bodies: at the crossroads of post-transcriptional pathways
Eulalio, A., Behm-Ansmant, I., & Izaurralde, E. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2080 · Cited by 936
Dynamic Shuttling of Tia-1 Accompanies the Recruitment of mRNA to Mammalian Stress Granules
Kedersha, N., Cho, M. R., Li, W., Yacono, P. W., Chen, S., Gilks, N., Golan, D. E., & Anderson, P. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.6.1257 · Cited by 814
Self-citationP-body formation is a consequence, not the cause, of RNA-mediated gene silencing
Eulalio, A., Behm-Ansmant, I., Schweizer, D., & Izaurralde, E. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.00128-07 · Cited by 710
Mammalian Stress Granules and Processing Bodies
Kedersha, N., & Anderson, P. (2007) · Methods in Enzymology · doi:10.1016/s0076-6879(07)31005-7 · Cited by 662
Self-citation
Cited by
Hart, G. W., Slawson, C., Ramirez-Correa, G., & Lagerlof, O. (2011) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060608-102511 · Cited by 1,317
G3BP1 is a tunable switch that triggers phase separation to assemble stress granules
Yang, P., Mathieu, C., Kolaitis, R.-M., Zhang, P., Messing, J., Yurtsever, U., Yang, Z., Wu, J., Li, Y., Pan, Q., et al. (2020) · Cell · doi:10.1016/j.cell.2020.03.046 · Cited by 1,249
Stress granules and neurodegeneration
Wolozin, B., & Ivanov, P. (2019) · Nature Reviews Neuroscience · doi:10.1038/s41583-019-0222-5 · Cited by 810
Distinct stages in stress granule assembly and disassembly
Wheeler, J. R., Matheny, T., Jain, S., Abrisch, R., & Parker, R. (2016) · eLife · doi:10.7554/elife.18413 · Cited by 804
Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization
Sanders, D. W., Kedersha, N., Lee, D. S. W., Strom, A. R., Drake, V., Riback, J. A., Bracha, D., Eeftens, J. M., Iwanicki, A., Wang, A., et al. (2020) · Cell · doi:10.1016/j.cell.2020.03.050 · Cited by 773
Self-citation
Metadata
- Authors
- Ohn, T., Kedersha, N., Hickman, T., Tisdale, S., & Anderson, P.
- Venue
- Nature Cell Biology
- Published
- 2008-09-14
- Volume
- 10
- Issue
- 10
- Pages
- 1224-1231
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2014241716
- Cited by
- 414
- References
- 35
- FWCI
- 8.56
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly. https://app.cochat.ai/papers/10.1038/ncb1783
- The paper
- Ohn, T., Kedersha, N., Hickman, T., Tisdale, S., & Anderson, P. (2008). A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly. Nature Cell Biology. https://doi.org/10.1038/ncb1783
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
