The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation
Open accessAbstract
Focal adhesions undergo myosin-II-mediated maturation wherein they grow and change composition to modulate integrin signalling for cell migration, growth and differentiation. To determine how focal adhesion composition is affected by myosin II activity, we performed proteomic analysis of isolated focal adhesions and compared protein abundance in focal adhesions from cells with and without myosin II inhibition. We identified 905 focal adhesion proteins, 459 of which changed in abundance with myosin II inhibition, defining the myosin-II-responsive focal adhesion proteome. The abundance of 73% of the proteins in the myosin-II-responsive focal adhesion proteome was enhanced by contractility, including proteins involved in Rho-mediated focal adhesion maturation and endocytosis- and calpain-dependent focal adhesion disassembly. During myosin II inhibition, 27% of proteins in the myosin-II-responsive focal adhesion proteome, including proteins involved in Rac-mediated lamellipodial protrusion, were enriched in focal adhesions, establishing that focal adhesion protein recruitment is also negatively regulated by contractility. We focused on the Rac guanine nucleotide exchange factor β-Pix, documenting its role in the negative regulation of focal adhesion maturation and the promotion of lamellipodial protrusion and focal adhesion turnover to drive cell migration.
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Cites
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“Forces on tension-sensitive focal adhesion proteins drive conformational changes that unmask binding sites for non-tension-sensitive protein”
BackgroundThe final steps of integrin activation: the end game
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Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck
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Cited by
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Winograd-Katz, S. E., Fässler, R., Geiger, B., & Legate, K. R. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3769 · Cited by 623
Horton, E. R., Byron, A., Askari, J. A., Ng, D. H. J., Millon-Frémillon, A., Robertson, J., Koper, E. J., Paul, N. R., Warwood, S., Knight, D., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3257 · Cited by 543
Case, L. B., & Waterman, C. M. (2015) · Nature Cell Biology · doi:10.1038/ncb3191 · Cited by 520
Self-citationBurgstaller, G., Oehrle, B., Gerckens, M., White, E. S., Schiller, H. B., & Eickelberg, O. (2017) · European Respiratory Journal · doi:10.1183/13993003.01805-2016 · Cited by 482
Focal adhesions, stress fibers and mechanical tension
Burridge, K., & Guilluy, C. (2016) · Experimental Cell Research · doi:10.1016/j.yexcr.2015.10.029 · Cited by 409
Metadata
- Authors
- Kuo, J.-C., Han, X., Hsiao, C.-T., Yates III, J. R., & Waterman, C. M.
- Venue
- Nature Cell Biology
- Published
- 2011-03-20
- Volume
- 13
- Issue
- 4
- Pages
- 383-393
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W1988194926
- Cited by
- 591
- References
- 7
- FWCI
- 23.55
- Type
- journal article
- Language
- en
- Open access
- green
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- Kuo, J.-C., Han, X., Hsiao, C.-T., Yates III, J. R., & Waterman, C. M. (2011). Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nature Cell Biology. https://doi.org/10.1038/ncb2216
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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