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Cited by 520Cellular Mechanics and InteractionsCell Adhesion Molecules ResearchForce Microscopy Techniques and Applications

Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch

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Case, Lindsay B., Waterman, Clare M.·2015·Nature Cell Biology
DOI 10.1038/ncb3191PMC PMC6300998
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Abstract

During cell migration, the forces generated in the actin cytoskeleton are transmitted across transmembrane receptors to the extracellular matrix or other cells through a series of mechanosensitive, regulable protein-protein interactions termed the molecular clutch. In integrin-based focal adhesions, the proteins forming this linkage are organized into a conserved three-dimensional nano-architecture. Here we discuss how the physical interactions between the actin cytoskeleton and focal-adhesion-associated molecules mediate force transmission from the molecular clutch to the extracellular matrix.

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  • Tensile force generated by polarized F-actin polymerization allows for integrin activation and focal adhesion maturation, both of which are necessary for cellular adhesion and migration through extracellular matrices.

    “Tensile force generated by polarized F-actin polymerization allows for integrin activation and focal adhesion maturation, both of which are necessary for cellular adhesion and migration through extracellular matrices (Fig. 1b) [18, 19].”

    From HEM1 Actin Immunodysregulatory Disorder: Genotypes, Phenotypes, and Future Directions (2022) · cites this paper as [18]

    In “The actin cytoskeleton is a master regulator of FAs” (page not given), the paper links actin flow to local integrin activation and says focal-adhesion maturation requires tension. It does not establish that polarized F-actin polymerization is the sole source of that tension: it also names myosin contractility and extracellular pulling. Its discussion of migration describes other mechanisms, so the claim that integrin activation and focal-adhesion maturation are necessary for migration through extracellular matrices is too broad. (LLM, conf=0.87)

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Cited by

Sorted by citations

Authors
Case, L. B., & Waterman, C. M.
Affiliations
National Institutes of Health (US)
Corresponding author
Waterman, Clare M.
ORCID
Venue
Nature Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2015-06-29
Volume
17
Issue
8
Pages
955-963
ISSN
  • 1465-7392 (ISSN-L)
  • 1476-4679
DOI
10.1038/ncb3191
PMC
PMC6300998
OpenAlex
W2211191608
Cited by
520
References
3
Citations by year
2026: 30 · 2025: 48 · 2024: 43 · 2023: 56 · 2022: 58 · 2021: 47 · 2020: 55 · 2019: 41 · 2018: 46 · 2017: 57 · 2016: 32 · 2015: 7
FWCI
4.78
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health
  • National Heart, Lung, and Blood Institute (Z01HL005105)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Keywords
molecular clutch, actin cytoskeleton, cell adhesion, integrins, focal adhesions, force transmission, extracellular matrix, cell migration
MeSH terms
Actins, Animals, Cell Adhesion, Cell Movement, Humans, Actin Cytoskeleton, Pressure, Time Factors, Integrins, Extracellular Matrix Proteins, Focal Adhesions, Mechanotransduction, Cellular

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