The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Differential requirement for the dual functions of β-catenin in embryonic stem cell self-renewal and germ layer formation
Open accessAbstract
Canonical Wnt signalling has been implicated in mouse and human embryonic stem cell (ESC) maintenance; however, its requirement is controversial. β-catenin is the key component in this highly conserved Wnt pathway, acting as a transcriptional transactivator. However, β-catenin has additional roles at the plasma membrane regulating cell–cell adhesion, complicating the analyses of cells/tissues lacking β-catenin. We report here the generation of a Ctnnb1 (β-catenin)-deficient mouse ESC (mESC) line and show that self-renewal is maintained in the absence of β-catenin. Cell adhesion is partially rescued by plakoglobin upregulation, but fails to be maintained during differentiation. When differentiated as aggregates, wild-type mESCs form descendants of all three germ layers, whereas mesendodermal germ layer formation and neuronal differentiation are defective in Ctnnb1-deficient mESCs. A Tcf/Lef-signalling-defective β-catenin variant, which re-establishes cadherin-mediated cell adhesion, rescues definitive endoderm and neuroepithelial formation, indicating that the β-catenin cell-adhesion function is more important than its signalling function for these processes.
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The ground state of embryonic stem cell self-renewal
Ying, Q.-L., Wray, J., Nichols, J., Batlle-Morera, L., Doble, B., Woodgett, J., Cohen, P., & Smith, A. (2008) · Nature · doi:10.1038/nature06968 · Cited by 3,442
Mao, J., Wang, J., Liu, B., Pan, W., Farr, G. H., Flynn, C., Yuan, H., Takada, S., Kimelman, D., Li, L., et al. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00224-6 · Cited by 839 · cited 1×
“Ligand-binding results in inhibition of the destruction complex composed out of Adenomatosis polyposis coli, Axin, Casein kinase 1 α (CK1 α ) and the Glycogen synthase kinase 3 β (GSK3 β ) 13, 14 .”
Apc modulates embryonic stem-cell differentiation by controlling the dosage of β-catenin signaling
Kielman, M. F., Rindapää, M., Gaspar, C., van Poppel, N., Breukel, C., van Leeuwen, S., Taketo, M. M., Roberts, S., Smits, R., & Fodde, R. (2002) · Nature Genetics · doi:10.1038/ng1045 · Cited by 362
Cited by
Kriks, S., Shim, J.-W., Piao, J., Ganat, Y. M., Wakeman, D. R., Xie, Z., Carrillo-Reid, L., Auyeung, G., Antonacci, C., Buch, A., et al. (2011) · Nature · doi:10.1038/nature10648 · Cited by 1,907
The many faces and functions of β‐catenin
Valenta, T., Hausmann, G., & Basler, K. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.150 · Cited by 1,598
Wnt signaling in development and tissue homeostasis
Steinhart, Z., & Angers, S. (2018) · Development · doi:10.1242/dev.146589 · Cited by 821
Mitotic and mitogenic Wnt signalling
Niehrs, C., & Acebron, S. P. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.124 · Cited by 305
Maintaining embryonic stem cell pluripotency with Wnt signaling
Sokol, S. Y. (2011) · Development · doi:10.1242/dev.066209 · Cited by 257
Metadata
- Authors
- Lyashenko, N., Winter, M., Migliorini, D., Biechele, T., Moon, R. T., & Hartmann, C.
- Venue
- Nature Cell Biology
- Published
- 2011-06-19
- Volume
- 13
- Issue
- 7
- Pages
- 753-761
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2041472984
- Cited by
- 241
- References
- 3
- FWCI
- 10.70
- Type
- journal article
- Language
- en
- Open access
- green
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