The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Extracellular rigidity sensing by talin isoform-specific mechanical linkages
Open accessAbstract
The ability of cells to adhere and sense differences in tissue stiffness is crucial for organ development and function. The central mechanisms by which adherent cells detect extracellular matrix compliance, however, are still unknown. Using two single-molecule-calibrated biosensors that allow the analysis of a previously inaccessible but physiologically highly relevant force regime in cells, we demonstrate that the integrin activator talin establishes mechanical linkages following cell adhesion, which are indispensable for cells to probe tissue stiffness. Talin linkages are exposed to a range of piconewton forces and bear, on average, 7–10 pN during cell adhesion depending on their association with F-actin and vinculin. Disruption of talin’s mechanical engagement does not impair integrin activation and initial cell adhesion but prevents focal adhesion reinforcement and thus extracellular rigidity sensing. Intriguingly, talin mechanics are isoform specific so that expression of either talin-1 or talin-2 modulates extracellular rigidity sensing.
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Citations
Cites
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
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Environmental sensing through focal adhesions
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Single myosin molecule mechanics: piconewton forces and nanometre steps
Finer, J. T., Simmons, R. M., & Spudich, J. A. (1994) · Nature · doi:10.1038/368113a0 · Cited by 1,915
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Nanoscale architecture of integrin-based cell adhesions
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Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
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Self-citationStretching Single Talin Rod Molecules Activates Vinculin Binding
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Cited by
Integrins as biomechanical sensors of the microenvironment
Kechagia, J. Z., Ivaska, J., & Roca-Cusachs, P. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0134-2 · Cited by 1,311
Elosegui-Artola, A., Oria, R., Chen, Y., Kosmalska, A., Pérez-González, C., Castro, N., Zhu, C., Trepat, X., & Roca-Cusachs, P. (2016) · Nature Cell Biology · doi:10.1038/ncb3336 · Cited by 858
Talin – the master of integrin adhesions
Klapholz, B., & Brown, N. H. (2017) · Journal of Cell Science · doi:10.1242/jcs.190991 · Cited by 309
Talin as a mechanosensitive signaling hub
Goult, B. T., Yan, J., & Schwartz, M. A. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201808061 · Cited by 250
FAK Structure and Regulation by Membrane Interactions and Force in Focal Adhesions
Tapial Martínez, P., López Navajas, P., & Lietha, D. (2020) · Biomolecules · doi:10.3390/biom10020179 · Cited by 206
“5–10 pN [80,81] with peak forces reaching ~40 pN for molecules engaged with integrin receptors [82].”
Background
Metadata
- Authors
- Austen, K., Ringer, P., Mehlich, A., Chrostek-Grashoff, A., Kluger, C., Klingner, C., Sabass, B., Zent, R., Rief, M., & Grashoff, C.
- Venue
- Nature Cell Biology
- Published
- 2015-11-02
- Volume
- 17
- Issue
- 12
- Pages
- 1597-1606
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2188169307
- Cited by
- 346
- References
- 58
- FWCI
- 15.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Austen, K., Ringer, P., Mehlich, A., Chrostek-Grashoff, A., Kluger, C., Klingner, C., Sabass, B., Zent, R., Rief, M., & Grashoff, C. (2015). Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nature Cell Biology. https://doi.org/10.1038/ncb3268
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