Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity
Abstract
Cell function depends on tissue rigidity, which cells probe by applying and transmitting forces to their extracellular matrix, and then transducing them into biochemical signals. Here we show that in response to matrix rigidity and density, force transmission and transduction are explained by the mechanical properties of the actin-talin-integrin-fibronectin clutch. We demonstrate that force transmission is regulated by a dynamic clutch mechanism, which unveils its fundamental biphasic force/rigidity relationship on talin depletion. Force transduction is triggered by talin unfolding above a stiffness threshold. Below this threshold, integrins unbind and release force before talin can unfold. Above the threshold, talin unfolds and binds to vinculin, leading to adhesion growth and YAP nuclear translocation. Matrix density, myosin contractility, integrin ligation and talin mechanical stability differently and nonlinearly regulate both force transmission and the transduction threshold. In all cases, coupling of talin unfolding dynamics to a theoretical clutch model quantitatively predicts cell response.
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ECM stiffness triggers mechanotransduction pathways that influence tumor cell behavior, including activation of YAP/TAZ, FAK, and MAPK pathways and modulation of gene expression associated with proliferation, EMT, and drug resistance.
“However, beyond acting as a physical barrier, ECM stiffness also triggers mechanotransduction pathways that profoundly influence tumor cell behavior [91,92]. Mechanical cues generated by a stiffened matrix are sensed through integrin-based focal adhesions and transmitted via cytoskeletal tension to activate intracellular signaling cascades, including Yes-associated protein/Transcriptional coactiv”
From Modeling Tumor Microenvironment Complexity In Vitro: Spheroids as Physiologically Relevant Tumor Models and Strategies for Their Analysis (2025) · cites this paper as [93]
The paper’s opening summary (p. 1) reports that matrix rigidity can trigger talin unfolding, adhesion growth, and YAP nuclear translocation; the Discussion (p. 9) likewise describes a rigidity threshold for YAP signaling. The study uses mouse embryonic fibroblasts, not tumor cells, and the cited passages do not establish activation of FAK or MAPK pathways or changes in genes associated with proliferation, EMT, or drug resistance. (LLM, conf=0.87)
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“(9) Destroy modules shorter than length lmin.”
MethodControl of Mechanotransduction by Molecular Clutch Dynamics
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“Recent experiments demonstrated that FAs grow on stiff substrates through the recruitment of integrins to the adhesion complex via talin unfolding (20, 21).”
BackgroundInfluentialMechanotransduction at the cell-matrix interface
Jansen, K. A., Atherton, P., & Ballestrem, C. (2017) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2017.07.027 · Cited by 271 · see citing works
Steps in Mechanotransduction Pathways that Control Cell Morphology
Wolfenson, H., Yang, B., & Sheetz, M. P. (2018) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021317-121245 · Cited by 262 · see citing works
“In recent studies (71), depletion of talin produced the predicted clutch behavior, but normal behavior required an increased number of bonds with rigidity.”
BackgroundMatrix viscoelasticity promotes liver cancer progression in the pre-cirrhotic liver
Fan, W., Adebowale, K., Váncza, L., Li, Y., Rabbi, M. F., Kunimoto, K., Chen, D., Mozes, G., Chiu, D. K.-C., Li, Y., et al. (2024) · Nature · doi:10.1038/s41586-023-06991-9 · Cited by 263 · see citing works
Different TCR-induced T lymphocyte responses are potentiated by stiffness with variable sensitivity
Saitakis, M., Dogniaux, S., Goudot, C., Bufi, N., Asnacios, S., Maurin, M., Randriamampita, C., Asnacios, A., & Hivroz, C. (2017) · eLife · doi:10.7554/elife.23190 · Cited by 253 · see citing works
“This might be due to the fact that force transduction depends on the mechanical properties of the actin–talin–integrin–ligand clutch which is triggered by talin unfolding above a stiffness threshold (Elosegui-Artola et al., 2016).”
BackgroundTalin as a mechanosensitive signaling hub
Goult, B. T., Yan, J., & Schwartz, M. A. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201808061 · Cited by 250 · see citing works
“The resultant tension on talin initially opens the R3 domain, which acts as a gatekeeper to adhesion assembly (Goult et al., 2013; Yao et al., 2014; Atherton et al., 2015; Elosegui-Artola et al., 2016).”
Optogenetic control of cellular forces and mechanotransduction
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Directed cell migration towards softer environments
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The Extracellular Matrix in Skin Inflammation and Infection
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Mechanical regulation of chromatin and transcription
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Receptor-mediated cell mechanosensing
Chen, Y., Ju, L. A., Rushdi, M. N., Ge, C., & Zhu, C. (2017) · Molecular Biology of the Cell · doi:10.1091/mbc.e17-04-0228 · Cited by 230 · see citing works
“…Sheetz, 2011; Wen et al., 2014) also depend on adhesion receptor–mediated mechanosensing, as evidenced by their sensitivity to substrate stiffness (Elosegui-Artola et al., 2016), which in population can even fulfill sophisticated tissue- and organ-level tasks like ECM remodeling (Cox and Erler,…”
BackgroundInfluentialStowers, R. S., Shcherbina, A., Israeli, J., Gruber, J. J., Chang, J., Nam, S., Rabiee, A., Teruel, M. N., Snyder, M. P., Kundaje, A. B., et al. (2019) · Nature Biomedical Engineering · doi:10.1038/s41551-019-0420-5 · Cited by 227 · see citing works
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“A recent study has shown the importance of talin integrin interactions in transmission of mechanical force in response to ECM rigidity (47).”
BackgroundERBB2 drives YAP activation and EMT-like processes during cardiac regeneration
Aharonov, A., Shakked, A., Umansky, K. B., Savidor, A., Genzelinakh, A., Kain, D., Lendengolts, D., Revach, O., Morikawa, Y., Dong, J., et al. (2020) · Nature Cell Biology · doi:10.1038/s41556-020-00588-4 · Cited by 214 · see citing works
“Likewise, analysis of the proteome revealed an elevation in several cytoplasmic membrane-bound and nuclear-envelope bound force sensors and transducers in OE hearts (Figure 4J) (Elosegui-artola et al., 2016).”
BackgroundMechanosensing through immunoreceptors
Zhu, C., Chen, W., Lou, J., Rittase, W., & Li, K. (2019) · Nature Immunology · doi:10.1038/s41590-019-0491-1 · Cited by 203 · see citing works
Nanopillar force measurements reveal actin-cap-mediated YAP mechanotransduction
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YAP and TAZ limit cytoskeletal and focal adhesion maturation to enable persistent cell motility
Mason, D. E., Collins, J. M., Dawahare, J. H., Nguyen, T. D., Lin, Y., Voytik‐Harbin, S. L., Zorlutuna, P., Yöder, M. C., & Boerckel, J. D. (2019) · The Journal of Cell Biology · doi:10.1083/jcb.201806065 · Cited by 189 · see citing works
“Migration is driven by actomyosin force generation, which coordinates focal adhesion (FA) formation, reinforcement, and disassembly (Chan and Odde, 2008; Elosegui-Artola et al., 2016; Wu et al., 2017).”
BackgroundExtracellular Matrices and Cancer-Associated Fibroblasts: Targets for Cancer Diagnosis and Therapy?
Belhabib, I., Zaghdoudi, S., Lac, C., Bousquet, C., & Jean, C. (2021) · Cancers · doi:10.3390/cancers13143466 · Cited by 196 · see citing works
“The increase in ECM stiffness induces in CAFs, an “outside-in” signaling via integrin engagement facilitating the formation of focal adhesions [133].”
BackgroundMesoscale physical principles of collective cell organization
Trepat, X., & Sahai, E. A. (2018) · Nature Physics · doi:10.1038/s41567-018-0194-9 · Cited by 188 · see citing works
Implant Fibrosis and the Underappreciated Role of Myofibroblasts in the Foreign Body Reaction
Noskovicova, N., Hinz, B., & Pakshir, P. (2021) · Cells · doi:10.3390/cells10071794 · Cited by 184 · see citing works
Mechanical force application to the nucleus regulates nucleocytoplasmic transport
Andreu, I., Granero-Moya, I., Chahare, N. R., Clein, K., Molina-Jordán, M., Beedle, A. E. M., Elósegui-Artola, A., Abenza, J. F., Rossetti, L., Trepat, X., et al. (2022) · Nature Cell Biology · doi:10.1038/s41556-022-00927-7 · Cited by 188 · see citing works
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Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1
Ringer, P., Weißl, A., Cost, A., Freikamp, A., Sabass, B., Mehlich, A., Tramier, M., Rief, M., & Grashoff, C. (2017) · Nature Methods · doi:10.1038/nmeth.4431 · Cited by 181 · see citing works
Herum, K. M., Lunde, I. G., McCulloch, A. D., & Christensen, G. (2017) · Journal of Clinical Medicine · doi:10.3390/jcm6050053 · Cited by 183 · see citing works
“Interestingly, it was recently shown that talin is necessary for sensing substrate stiffness [150].”
BackgroundExtracellular Matrix Remodeling in Chronic Liver Disease
Ortiz, C., Schierwagen, R., Schaefer, L., Klein, S., Trepat, X., & Trebicka, J. (2021) · Current Tissue Microenvironment Reports · doi:10.1007/s43152-021-00030-3 · Cited by 183 · see citing works
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Molecular clutch drives cell response to surface viscosity
Bennett, M., Cantini, M., Reboud, J., Cooper, J. M., Roca‐Cusachs, P., & Salmerón‐Sánchez, M. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1710653115 · Cited by 171 · see citing works
“Previous work has shown that VD1 acts as a dominant negative for the binding to talin of endogenous vinculin, preventing the stiffness response mediated by talin unfolding (17).”
BackgroundMethodInfluentialDesigning Hydrogels for 3D Cell Culture Using Dynamic Covalent Crosslinking
Rizwan, M., Baker, A. E. G., & Shoichet, M. S. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202100234 · Cited by 171 · see citing works
Luo, J., Walker, M., Xiao, Y., Donnelly, H., Dalby, M. J., & Salmerón‐Sánchez, M. (2021) · Bioactive Materials · doi:10.1016/j.bioactmat.2021.11.024 · Cited by 169 · see citing works
“The general view is that initial, nascent adhesions form independently of force and then mature in response to force applied either internally by actin structures coupled to integrins at nascent adhesions, or by any of the external factors through the ECM [149,156,167,168].”
BackgroundInfluentialIntegrin signaling: linking mechanical stimulation to skeletal muscle hypertrophy
Boppart, M. D., & Mahmassani, Z. S. (2019) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00009.2019 · Cited by 157 · see citing works
Cancer associated fibroblasts: is the force the path to the dark side?
Barbazán, J., & Vignjevic, D. M. (2018) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2018.09.002 · Cited by 158 · see citing works
Regulation of Hippo signaling by actin remodeling
Seo, J., & Kim, J. (2018) · BMB Reports · doi:10.5483/bmbrep.2018.51.3.012 · Cited by 159 · see citing works
“In this context, YAP/TAZ nuclear translocation is enhanced (21, 22).”
BackgroundA glance on the role of actin in osteogenic and adipogenic differentiation of mesenchymal stem cells
Khan, A. U., Qu, R., Fan, T., Ouyang, J., & Dai, J. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-01789-2 · Cited by 160 · see citing works
Actin-based force generation and cell adhesion in tissue morphogenesis
Clarke, D. N., & Martin, A. C. (2021) · Current Biology · doi:10.1016/j.cub.2021.03.031 · Cited by 159 · see citing works
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Mechanosensing through YAP controls T cell activation and metabolism
Meng, K., Majedi, F. S., Thauland, T. J., & Butte, M. J. (2020) · The Journal of Experimental Medicine · doi:10.1084/jem.20200053 · Cited by 144 · see citing works
Measuring mechanical stress in living tissues
Gómez‐González, M., Latorre, E., Arroyo, M., & Trepat, X. (2020) · Nature Reviews Physics · doi:10.1038/s42254-020-0184-6 · Cited by 147 · see citing works
Extracellular Matrix Cues Regulate Mechanosensing and Mechanotransduction of Cancer Cells
Mierke, C. T. (2024) · Cells · doi:10.3390/cells13010096 · Cited by 146 · see citing works
Material approaches to active tissue mechanics
Xi, W., Saw, T. B., Delacour, D., Lim, C. T., & Ladoux, B. (2018) · Nature Reviews Materials · doi:10.1038/s41578-018-0066-z · Cited by 145 · see citing works
Jaumouillé, V., Cartagena‐Rivera, A. X., & Waterman, C. M. (2019) · Nature Cell Biology · doi:10.1038/s41556-019-0414-2 · Cited by 140 · see citing works
Integrin diversity brings specificity in mechanotransduction
Seetharaman, S., & Etienne‐Manneville, S. (2018) · Biology of the Cell · doi:10.1111/boc.201700060 · Cited by 140 · see citing works
“In general, the key event leading to the recruitment of focal adhesion proteins is the mechanical stretching of the talin rod which activates vinculin binding to the vinculin binding sites (VBS) located in the carboxy-terminal domain of talin [del Rio et al., 2009; Elosegui-Artola et al., 2016].”
BackgroundIntegrin and cadherin clusters: A robust way to organize adhesions for cell mechanics
Changede, R., & Sheetz, M. P. (2016) · BioEssays · doi:10.1002/bies.201600123 · Cited by 130 · see citing works
“of behavior was observed only after talin depletion [116].”
BackgroundLive-cell super-resolved PAINT imaging of piconewton cellular traction forces
Brockman, J. M., Su, H., Blanchard, A. T., Duan, Y., Meyer, T. A., Quach, M. E., Glazier, R., Bazrafshan, A. S., Bender, R. L., Kellner, A. V., et al. (2020) · Nature Methods · doi:10.1038/s41592-020-0929-2 · Cited by 136 · see citing works
Mekhdjian, A. H., Kai, F., Rubashkin, M. G., Prahl, L. S., Przybyla, L., McGregor, A., Bell, E. S., Barnes, J. M., DuFort, C. C., Ou, G., et al. (2017) · Molecular Biology of the Cell · doi:10.1091/mbc.e16-09-0654 · Cited by 133 · see citing works
“We developed a modified motor-clutch model that includes force-dependent recruitment of adhesion components in response to substrate stiffness, similar to recently proposed models (Elosegui-Artola et al., 2014, 2016).”
BackgroundMethodArgentati, C., Morena, F., Tortorella, I., Bazzucchi, M., Porcellati, S., Emiliani, C., & Martino, S. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20215337 · Cited by 136 · see citing works
Lamellipodium is a myosin-independent mechanosensor
Oakes, P. W., Bidone, T. C., Beckham, Y., Skeeters, A. V., Juan, G. R. R.-S., Winter, S. P., Voth, G. A., & Gardel, M. L. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1715869115 · Cited by 133 · see citing works
“Conceptually, this framework is similar to the general motorclutch model (54) that has been suggested as a mechanism for understanding mechanosensitivity (23, 24, 26, 27).”
ResultExtracellular matrix type modulates mechanotransduction of stem cells
Stanton, A. E., Tong, X., & Yang, F. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.06.048 · Cited by 132 · see citing works
“To characterize YAP localization in a quantitative manner, we employed a method [21] which reports the ratio of nuclear YAP intensity vs.”
MethodAdjustable viscoelasticity allows for efficient collective cell migration
Barriga, E. H., & Mayor, R. (2018) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2018.05.027 · Cited by 130 · see citing works
d’Angelo, M., Benedetti, E., Tupone, M. G., Catanesi, M., Castelli, V., Antonosante, A., & Cimini, A. (2019) · Cells · doi:10.3390/cells8091036 · Cited by 132 · see citing works
Cell monolayers sense curvature by exploiting active mechanics and nuclear mechanoadaptation
Luciano, M., Xue, S.-L., Vos, W. H. D., Redondo‐Morata, L., Surin, M., Lafont, F., Hannezo, É., & Gabriele, S. (2021) · Nature Physics · doi:10.1038/s41567-021-01374-1 · Cited by 128 · see citing works
Actomyosin contractility-dependent matrix stretch and recoil induces rapid cell migration
Wang, W. Y., Davidson, C. D., Lin, D., & Baker, B. M. (2019) · Nature Communications · doi:10.1038/s41467-019-09121-0 · Cited by 126 · see citing works
Fiore, V. F., Wong, S. S., Tran, C., Tan, C., Xu, W., Sulchek, T., White, E. S., Hagood, J. S., & Barker, T. H. (2018) · JCI Insight · doi:10.1172/jci.insight.97597 · Cited by 121 · see citing works
Andreu, I., Falcones, B., Hurst, S., Chahare, N. R., Quiroga, X., Roux, A. L., Kechagia, Z., Beedle, A. E. M., Elósegui-Artola, A., Trepat, X., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-24383-3 · Cited by 121 · see citing works
Graham, D. M., Andersen, T., Sharek, L., Uzer, G., Rothenberg, K. E., Hoffman, B. D., Rubin, J. E., Balland, M., Bear, J. E., & Burridge, K. (2018) · The Journal of Cell Biology · doi:10.1083/jcb.201706097 · Cited by 121 · see citing works
“During the past few years, much has been learned about how tension exerted on the clutch affects the properties and interactions of components mediating adhesion (Elosegui-Artola et al., 2016).”
BackgroundPutra, V. D. L., Kilian, K. A., & Tate, M. L. K. (2023) · Communications Biology · doi:10.1038/s42003-022-04320-w · Cited by 121 · see citing works
The Plot Thickens: The Emerging Role of Matrix Viscosity in Cell Mechanotransduction
Cantini, M., Donnelly, H., Dalby, M. J., & Salmerón‐Sánchez, M. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201901259 · Cited by 116 · see citing works
Miller, A. E., Hu, P., & Barker, T. H. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.201901445 · Cited by 114 · see citing works
Single Molecule Force Measurements in Living Cells Reveal a Minimally Tensioned Integrin State
Chang, A. C., Mekhdjian, A. H., Morimatsu, M., Denisin, A. K., Pruitt, B. L., & Dunn, A. R. (2016) · ACS Nano · doi:10.1021/acsnano.6b03314 · Cited by 115 · see citing works
Extracellular fluid viscosity enhances liver cancer cell mechanosensing and migration
Gonzalez‐Molina, J., Zhang, X., Borghesan, M., da Silva, J. M., Awan, M., Fuller, B. J., Gavara, N., & Selden, C. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.05.058 · Cited by 115 · see citing works
“This model explains rigidity sensing considering the differences in integrin-ECM bond lifetime and force loading rates in soft and stiff substrates determining force transmission to the intracellular protein talin, which leads to adhesion reinforcement and mechanotransduction pathway activation [24].”
BackgroundMechanics in the nervous system: From development to disease
Pillai, E. K., & Franze, K. (2023) · Neuron · doi:10.1016/j.neuron.2023.10.005 · Cited by 116 · see citing works
“58 Furthermore, integrins form catch bonds with their ECM binding partners, 59 and the life-times of these bonds increase with the tensile force applied.”
Cao, X. D., Ban, E., Baker, B. M., Lin, Y., Burdick, J. A., Chen, C. S., & Shenoy, V. B. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1620486114 · Cited by 109 · see citing works
“It is well known that the bonds formed between integrins and ECM are highly dynamic (9, 20, 40).”
BackgroundInfluentialExtracellular matrix plasticity as a driver of cell spreading
Grolman, J. M., Weinand, P., & Mooney, D. J. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2008801117 · Cited by 113 · see citing works
“These cues include cell–cell interactions (5), soluble signaling molecules (6), and adhesion to the extracellular matrix (ECM) via transmembrane receptors (7, 8); the physical properties of the ECM, particularly its stiffness (9) and rate of stress relaxation, are known to regulate the ability of a variety of cell typ…”
BackgroundResultZhou, D. W., Lee, T. T., Weng, S., Fu, J., & Garcı́a, A. J. (2017) · Molecular Biology of the Cell · doi:10.1091/mbc.e17-02-0116 · Cited by 112 · see citing works
“Indeed, this is consistent with recent work showing that increases in substrate stiffness promote talin’s ability to unfold and bind vinculin to promote force transmission (Elosegui-Artola et al., 2016).”
BackgroundExtracellular Matrix Components and Mechanosensing Pathways in Health and Disease
Berdiaki, A., Neagu, M. T., Tzanakakis, P., Spyridaki, I., Pérez, S., & Nikitovic, D. (2024) · Biomolecules · doi:10.3390/biom14091186 · Cited by 116 · see citing works
Substrate mechanics controls adipogenesis through YAP phosphorylation by dictating cell spreading
Cruz, J. O. L., Nardone, G., Vrbský, J., Pompeiano, A., Perestrelo, A. R., Capradossi, F., Melajová, K., Filipenský, P., & Forte, G. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.03.009 · Cited by 110 · see citing works
“Hydrogels of di ff erent sti ff nesses were obtained by mixing di ff erent proportions of thrombin and fi brinogen according to previously published data [27], in order to produce compliant (300 Pa) and sti ff (5 kPa) hydrogels able to di ff erentially control YAP nuclear localization [56].”
Chirality Controls Mesenchymal Stem Cell Lineage Diversification through Mechanoresponses
Wei, Y. Q., Jiang, S., Si, M., Zhang, X., Liu, J., Wang, Z., Cao, C., Huang, J., Huang, H., Chen, L., et al. (2019) · Advanced Materials · doi:10.1002/adma.201900582 · Cited by 110 · see citing works
Hippo signalling during development
Davis, J. R., & Tapon, N. (2019) · Development · doi:10.1242/dev.167106 · Cited by 108 · see citing works
“When cells are cultured on soft substrates (<3 kPa) YAP/TAZ are cytoplasmic; however, their nuclear localisation increases when cultured on rigid substrates (>10 kPa) (Dupont et al., 2011; Elosegui-Artola et al., 2016).”
BackgroundTraction force microscopy – Measuring the forces exerted by cells
Lekka, M., Gnanachandran, K., Kubiak, A., Zieliński, T., & Zemła, J. (2021) · Micron · doi:10.1016/j.micron.2021.103138 · Cited by 108 · see citing works
Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable Effective Directed Cell Migration
Rothenberg, K. E., Scott, D. W., Christoforou, N., & Hoffman, B. D. (2018) · Biophysical Journal · doi:10.1016/j.bpj.2018.02.019 · Cited by 108 · see citing works
“Based on these results, it has been suggested that either talin, vinculin, or the interaction of talin and vinculin, is the key component of the molecular clutch or the crucial element in force-induced FA strengthening (15,25,30,45,47).”
BackgroundResultCell–extracellular matrix dynamics
Doyle, A. D., Nazari, S., & Yamada, K. M. (2021) · Physical Biology · doi:10.1088/1478-3975/ac4390 · Cited by 104 · see citing works
Actin polymerization downstream of integrins: signaling pathways and mechanotransduction
Romero, S., Clainche, C. L., & Gautreau, A. M. (2020) · Biochemical Journal · doi:10.1042/bcj20170719 · Cited by 105 · see citing works
Integrating Chemistry and Mechanics: The Forces Driving Axon Growth
Franze, K. (2020) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100818-125157 · Cited by 106 · see citing works
- Authors
- Elosegui-Artola, A., Oria, R., Chen, Y., Kosmalska, A., Pérez-González, C., Castro, N., Zhu, C., Trepat, X., & Roca-Cusachs, P.
- Affiliations
- Institute for Bioengineering of Catalonia (ES)
- Georgia Institute of Technology (US)
- Institució Catalana de Recerca i Estudis Avançats (ES)
- Venue
- Nature Cell Biology
- Published
- 2016-04-11
- Volume
- 18
- Issue
- 5
- Pages
- 540-548
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2327893020
- Cited by
- 858
- References
- 57
- Citations by year
- 2026: 72 · 2025: 101 · 2024: 87 · 2023: 93 · 2022: 103 · 2021: 93 · 2020: 96 · 2019: 71 · 2018: 67 · 2017: 60 · 2016: 15
- FWCI
- 48.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Dipòsit Digital de la Universitat de Barcelona (Universitat de Barcelona) (submittedVersion, public-domain)
- Funders
- National Institute of Allergy and Infectious Diseases (R01AI044902)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Keywords
- molecular clutch, matrix rigidity, force transmission, mechanotransduction, vinculin, YAP nuclear translocation, cell adhesion, extracellular matrix stiffness, myosin contractility, actin cytoskeleton, fibronectin
- MeSH terms
- YAP-Signaling Proteins, Animals, Biomechanical Phenomena, Cell Adhesion, Cell Nucleus, Cytoskeleton, Extracellular Matrix, Fibronectins, Phosphoproteins, Phosphorylation, Protein Binding, Integrins, Vinculin, Talin, Cell Cycle Proteins, Protein Transport, Stress Fibers, Mechanotransduction, Cellular, Adaptor Proteins, Signal Transducing, Mice, Focal Adhesion Protein-Tyrosine Kinases
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