Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits
Abstract
As a final step in endocytosis, clathrin-coated pits must separate from the plasma membrane and move into the cytosol as a coated vesicle. Because these events involve minute movements that conventional light microscopy cannot resolve, they have not been observed directly and their dynamics remain unexplored. Here, we used evanescent field (EF) microscopy to observe single clathrin-coated pits or vesicles as they draw inwards from the plasma membrane and finally lose their coats. This inward movement occurred immediately after a brief burst of dynamin recruitment and was accompanied by transient actin assembly. Therefore, dynamin may provide the trigger and actin may provide the force for movement into the cytosol.
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Endocytosis typically requires force generation by active actin polymerization at the maturing endocytic pit.
“Endocytosis typically requires force generation by active actin polymerization at the maturing endocytic pit52–57.”
From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [57]
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- Authors
- Merrifield, C. J., Feldman, M. E., Wan, L., & Almers, W.
- Affiliations
- Oregon Health & Science University (US)
- Corresponding author
- Almers, Wolfhard
- ORCID
- Wan, Lei
- Venue
- Nature Cell Biology
- Published
- 2002-08-27
- Volume
- 4
- Issue
- 9
- Pages
- 691-698
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2024316397
- Cited by
- 691
- References
- 0
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 7 · 2023: 10 · 2022: 18 · 2021: 17 · 2020: 16 · 2019: 11 · 2018: 34 · 2017: 13 · 2016: 26 · 2015: 16 · 2014: 39 · 2013: 31 · 2012: 35
- FWCI
- 16.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Mental Health (MH60600, R01MH060600)
- National Institute of Diabetes and Digestive and Kidney Diseases (P01DK044239, DK44239)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Keywords
- clathrin-coated pits, coated vesicle formation, endocytosis, actin assembly, single-particle imaging, membrane fission
- MeSH terms
- Actins, Animals, Clathrin, Coated Pits, Cell-Membrane, Cytoskeleton, Endocytosis, Kinetics, Microscopy, Fluorescence, Models, Biological, Movement, 3T3 Cells, GTP Phosphohydrolases, Dynamins, Mice
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