Focusing on clathrin-mediated endocytosis.
Abstract
Investigations into the mechanisms which regulate entry of integral membrane proteins, and associated ligands, into the cell through vesicular carriers (endocytosis) have greatly benefited from the application of live-cell imaging. Several excellent recent reviews have detailed specific aspects of endocytosis, such as entry of particular cargo, or the different routes of internalization. The aim of the present review is to highlight how advances in live-cell fluorescence microscopy have affected the study of clathrin-mediated endocytosis. The last decade has seen a tremendous increase in the development and dissemination of methods for imaging endocytosis in live cells, and this has been followed by a dramatic shift in the way this critical cellular pathway is studied and understood. The present review begins with a description of the technical advances which have permitted new types of experiment to be performed, as well as potential pitfalls of these new technologies. Subsequently, advances in the understanding of three key endocytic proteins will be addressed: clathrin, dynamin and AP-2 (adaptor protein 2). Although great strides have clearly been made in these areas in recent years, as is often the case, each answer has bred numerous questions. Furthermore, several examples are highlighted where, because of seemingly minor differences in experimental systems, what appear at first to be very similar studies have, at times, yielded vastly differing results and conclusions. Thus this is an exceedingly exciting time to study endocytosis, and this area serves as a clear demonstration of the power of applying live-cell imaging to answer fundamental biological questions.
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Nano-delivery systems of size 100-200 nm can be easily accommodated in transport vesicles for receptor-mediated internalization and vesicular transport.
“Receptor-mediated internalization of cargo followed by vesicular transport is a suitable option as nano-delivery systems of size 100–200 nm range can be easily accommodated in these transport vesicles”
From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Rappoport, 2008
Clathrin-mediated endocytosis happens in all mammalian cells.
“Clathrin-mediated endocytosis happens in all mammalian cells.”
From Nanotechnology: A Promising Approach for Delivery of Neuroprotective Drugs (2020) · cites this paper as Rappoport, 2008
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Chen, J., Feng, X., & Dong, M. (2015) · Europe PMC (PubMed Central) · Cited by 15 · see citing works
Otake, H., Goto, R., Ogata, F., Isaka, T., Kawasaki, N., Kobayakawa, S., Matsunaga, T., & Nagai, N. (2021) · International Journal of Nanomedicine · doi:10.2147/ijn.s317046 · Cited by 15 · see citing works
- Authors
- Rappoport, J. Z.
- Affiliations
- University of Birmingham (GB)
- Corresponding author
- Rappoport, Joshua Z.
- ORCID
- Rappoport, Joshua Z.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 2008-05-28
- Volume
- 412
- Issue
- 3
- Pages
- 415-423
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W2012515487
- Cited by
- 172
- References
- 103
- Citations by year
- 2025: 4 · 2024: 8 · 2023: 5 · 2022: 6 · 2021: 6 · 2020: 14 · 2019: 6 · 2018: 5 · 2017: 16 · 2016: 11 · 2015: 10 · 2014: 12 · 2013: 9 · 2012: 14
- FWCI
- 1.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical Journal (publishedVersion)
- PubMed (publishedVersion)
- University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Keywords
- clathrin-mediated endocytosis, live-cell fluorescence microscopy, clathrin, dynamin, vesicle trafficking, endocytic proteins
- MeSH terms
- Animals, Clathrin, Endocytosis, Humans, Microscopy, Fluorescence, Protein Transport, Clathrin-Coated Vesicles, Adaptor Protein Complex 2, Dynamins
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