Fate of micelles and quantum dots in cells
Abstract
Micelles and quantum dots have been used as experimental drug delivery systems and imaging tools both in vitro and in vivo. Investigations of their fate at the subcellular level require different surface-core modifications. Among the most common modifications are those with fluorescent probes, dense-core metals or radionucleids. Cellular fate of several fluorescent probes incorporated into poly(caprolactone)-b-copolymer micelles (PCL-b-PEO) was followed by confocal microscopy, and colloidal gold incorporated in poly 4-vinyl pyridine-PEO micelles were developed to explore micelle fate by electron microscopy. More recently, we have examined quantum dots (QDs) as the next-generation-labels for cells and nanoparticulate drug carriers amenable both to confocal and electron microscopic analyses. Effects of QDs at the cellular and subcellular levels and their integrity were studied. Results from different studies suggest that size, charge and surface manipulations of QDs may play a role in their subcellular distribution. Examples of pharmacological agents incorporated into block copolymer micelles, administered or attached to QD surfaces show how the final biological outcome (e.g. cell death, proliferation or differentiation) depends on physical properties of these nanoparticles.
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Ghezzi, M., Pescina, S., Padula, C., Santi, P., Del Favero, E., Cantù, L., & Nicoli, S. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.02.031 · Cited by 1,135 · see citing works
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- Authors
- Maysinger, D., Lovrić, J., Eisenberg, A., & Savić, R.
- Affiliations
- University of Zagreb (HR)
- McGill University (CA)
- Venue
- European Journal of Pharmaceutics and Biopharmaceutics
- Publisher
- Elsevier BV
- Published
- 2007-03
- Volume
- 65
- Issue
- 3
- Pages
- 270-281
- ISSN
- 0939-6411 (ISSN-L)
- OpenAlex
- W1964960898
- Cited by
- 160
- References
- 136
- Citations by year
- 2025: 4 · 2024: 7 · 2023: 8 · 2022: 3 · 2021: 4 · 2020: 6 · 2019: 3 · 2018: 2 · 2017: 6 · 2016: 8 · 2015: 5 · 2014: 6 · 2013: 12 · 2012: 14
- FWCI
- 0.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Pharmaceutics and Biopharmaceutics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Canadian Institutes of Health Research
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Materials Science › Materials Chemistry › Quantum Dots Synthesis And Properties
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- micelles, quantum dots, subcellular localization, block copolymer micelles, gold nanoparticles, fluorescent probes, radionuclides, nanoparticle surface modification, nanoparticle drug carriers, cell death, cell proliferation, cell differentiation
- MeSH terms
- Animals, Cell Membrane, Cells, Cultured, Drug Carriers, Endocytosis, Ethylene Oxide, Fluorescent Dyes, Humans, Lactones, Micelles, Microscopy, Electron, Polyvinyls, Staining and Labeling, Cell Death, Reactive Oxygen Species, Gold Colloid, Oxidative Stress, Microscopy, Confocal, Fluorescence Resonance Energy Transfer, Quantum Dots
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