The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
A small molecule that directs differentiation of human ESCs into the pancreatic lineage
Open accessAbstract
Stepwise differentiation from embryonic stem cells (ESCs) to functional insulin-secreting beta cells will identify key steps in beta-cell development and may yet prove useful for transplantation therapy for diabetics. An essential step in this schema is the generation of pancreatic progenitors--cells that express Pdx1 and produce all the cell types of the pancreas. High-content chemical screening identified a small molecule, (-)-indolactam V, that induces differentiation of a substantial number of Pdx1-expressing cells from human ESCs. The Pdx1-expressing cells express other pancreatic markers and contribute to endocrine, exocrine and duct cells, in vitro and in vivo. Further analyses showed that (-)-indolactam V works specifically at one stage of pancreatic development, inducing pancreatic progenitors from definitive endoderm. This study describes a chemical screening platform to investigate human ESC differentiation and demonstrates the generation of a cell population that is a key milepost on the path to making beta cells.
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Cites
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Metadata
- Authors
- Chen, S., Borowiak, M., Fox, J. L., Maehr, R., Osafune, K., Davidow, L., Lam, K., Peng, L. F., Schreiber, S. L., Rubin, L. L., & Melton, D.
- Venue
- Nature Chemical Biology
- Published
- 2009-03-15
- Volume
- 5
- Issue
- 4
- Pages
- 258-265
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- OpenAlex
- W2169191544
- Cited by
- 478
- References
- 43
- FWCI
- 57.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Chen, S., Borowiak, M., Fox, J. L., Maehr, R., Osafune, K., Davidow, L., Lam, K., Peng, L. F., Schreiber, S. L., Rubin, L. L., & Melton, D. (2009). A small molecule that directs differentiation of human ESCs into the pancreatic lineage. Nature Chemical Biology. https://doi.org/10.1038/nchembio.154
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
