The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
Open accessAbstract
Targeted DNA double-strand breaks have been shown to significantly increase the frequency and precision of genome editing. In the past two decades, several double-strand break technologies have been developed. CRISPR–Cas9 has quickly become the technology of choice for genome editing due to its simplicity, efficiency and versatility. Currently, genome editing in plants primarily relies on delivering double-strand break reagents in the form of DNA vectors. Here we report biolistic delivery of pre-assembled Cas9–gRNA ribonucleoproteins into maize embryo cells and regeneration of plants with both mutated and edited alleles. Using this method of delivery, we also demonstrate DNA- and selectable marker-free gene mutagenesis in maize and recovery of plants with mutated alleles at high frequencies. These results open new opportunities to accelerate breeding practices in a wide variety of crop species.
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Cites
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The new frontier of genome engineering with CRISPR-Cas9
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Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation
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“In these experiments, DNA vectors encoding ‘helper genes’—cell division promoting transcription factors (maize ovule developmental protein 2 ( ODP2 ) and maize Wuschel ( WUS ) 21 ), and selectable and visible marker genes ( MOPAT-DSRED fusion) were co-bombarded with the RNPs.”
Self-citationPrecision genome engineering and agriculture: opportunities and regulatory challenges
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Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq
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Cited by
Breeding crops to feed 10 billion
Hickey, L. T., N. Hafeez, A., Robinson, H., Jackson, S. A., Leal-Bertioli, S. C. M., Tester, M., Gao, C., Godwin, I. D., Hayes, B. J., & Wulff, B. B. H. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0152-9 · Cited by 1,046
Zhang, Y., Bai, Y., Wu, G., Zou, S., Chen, Y., Gao, C., & Tang, D. (2017) · The Plant Journal · doi:10.1111/tpj.13599 · Cited by 525
Enhanced rice salinity tolerance via CRISPR/Cas9-targeted mutagenesis of the OsRR22 gene
Zhang, A., Liu, Y., Wang, F., Li, T., Chen, Z., Kong, D., Bi, J., Zhang, F., Luo, X., Wang, J., et al. (2019) · Molecular Breeding · doi:10.1007/s11032-019-0954-y · Cited by 507
Genome editing in potato via CRISPR‐Cas9 ribonucleoprotein delivery
Andersson, M., Turesson, H., Olsson, N., Fält, A., Ohlsson, P., Gonzalez, M. N., Samuelsson, M., & Hofvander, P. (2018) · Physiologia Plantarum · doi:10.1111/ppl.12731 · Cited by 432
Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
Zhang, S., Shen, J., Li, D., & Cheng, Y. (2021) · Theranostics · doi:10.7150/thno.47007 · Cited by 430
Metadata
- Authors
- Svitashev, S., Schwartz, C., Lenderts, B., Young, J. K., & Mark Cigan, A.
- Venue
- Nature Communications
- Published
- 2016-11-16
- Volume
- 7
- Issue
- 1
- Pages
- 13274-13274
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2551575280
- Cited by
- 824
- References
- 9
- FWCI
- 33.86
- Type
- journal article
- Language
- en
- Open access
- gold
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- Svitashev, S., Schwartz, C., Lenderts, B., Young, J. K., & Mark Cigan, A. (2016). Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes. Nature Communications. https://doi.org/10.1038/ncomms13274
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