Simultaneous modification of three homoeologs of Ta EDR 1 by genome editing enhances powdery mildew resistance in wheat
Open accessAbstract
Wheat (Triticum aestivum L.) incurs significant yield losses from powdery mildew, a major fungal disease caused by Blumeria graminis f. sp. tritici (Bgt). enhanced disease resistance1 (EDR1) plays a negative role in the defense response against powdery mildew in Arabidopsis thaliana; however, the edr1 mutant does not show constitutively activated defense responses. This makes EDR1 an ideal target for approaches using new genome-editing tools to improve resistance to powdery mildew. We cloned TaEDR1 from hexaploid wheat and found high similarity among the three homoeologs of EDR1. Knock-down of TaEDR1 by virus-induced gene silencing or RNA interference enhanced resistance to powdery mildew, indicating that TaEDR1 negatively regulates powdery mildew resistance in wheat. We used CRISPR/Cas9 technology to generate Taedr1 wheat plants by simultaneous modification of the three homoeologs of wheat EDR1. No off-target mutations were detected in the Taedr1 mutant plants. The Taedr1 plants were resistant to powdery mildew and did not show mildew-induced cell death. Our study represents the successful generation of a potentially valuable trait using genome-editing technology in wheat and provides germplasm for disease resistance breeding.
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CRISPR variants Cas12 and Cas13 offered improvements in specificity and reduced off-target effects compared to Cas9.
“The CRISPR variants Cas12 and Cas13 offered improvements in specificity and reduced off-target effects compared to Cas9 and proved to be a highly efficient gene editing tool with complementary properties and functionality to Cas9 (Zetsche et al., 2017; Zhang et al., 2017).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Zhang et al., 2017
The section on knocking out TaEDR1 with CRISPR/Cas9 reports no mutations at seven predicted off-target sites in its Cas9-edited wheat plants. Our copy does not address Cas12 or Cas13 or compare their specificity and off-target effects with Cas9 (searched: Cas12, Cas13, Cpf1, and complementary properties). (LLM, conf=0.78)
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- Authors
- Zhang, Y., Bai, Y., Wu, G., Zou, S., Chen, Y., Gao, C., & Tang, D.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Gao, Caixia
- Tang, Dingzhong
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2017-06-13
- Volume
- 91
- Issue
- 4
- Pages
- 714-724
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2612614002
- Cited by
- 525
- References
- 60
- Citations by year
- 2026: 35 · 2025: 59 · 2024: 66 · 2023: 75 · 2022: 100 · 2021: 67 · 2020: 59 · 2019: 37 · 2018: 19 · 2017: 6
- FWCI
- 32.56
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Chinese Academy of Sciences (XDB11020100, KFZD‐SW‐107)
- Ministry of Science and Technology of the People's Republic of China (2014DFA31540, 2015CB910202)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Powdery Mildew Fungal Diseases
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- homoeologs, wheat, powdery mildew resistance, Blumeria graminis f. sp. tritici, CRISPR/Cas9 genome editing, disease resistance breeding, virus-induced gene silencing, RNA interference
- MeSH terms
- Gene Editing, Amino Acid Sequence, Ascomycota, Plant Diseases, Polyploidy, Triticum, Sequence Alignment, Plant Leaves, Gene Silencing, Disease Resistance, Clustered Regularly Interspaced Short Palindromic Repeats
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