The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Five colour variants of bright luminescent protein for real-time multicolour bioimaging
Open accessAbstract
Luminescence imaging has gained attention as a promising bio-imaging modality in situations where fluorescence imaging cannot be applied. However, wider application to multicolour and dynamic imaging is limited by the lack of bright luminescent proteins with emissions across the visible spectrum. Here we report five new spectral variants of the bright luminescent protein, enhanced Nano-lantern (eNL), made by concatenation of the brightest luciferase, NanoLuc, with various colour hues of fluorescent proteins. eNLs allow five-colour live-cell imaging, as well as detection of single protein complexes and even single molecules. We also develop an eNL-based Ca 2+ indicator with a 500% signal change, which can image spontaneous Ca 2+ dynamics in cardiomyocyte and neural cell models. These eNL probes facilitate not only multicolour imaging in living cells but also sensitive imaging of a wide repertoire of proteins, even at very low expression levels.
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Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004) · Nature Biotechnology · doi:10.1038/nbt1037 · Cited by 4,619
Grimm, D., Lee, J. S., Wang, L., Desai, T., Akache, B., Storm, T. A., & Kay, M. A. (2008) · Journal of Virology · doi:10.1128/jvi.00254-08 · Cited by 696
Molecular model for a complete clathrin lattice from electron cryomicroscopy
Fotin, A., Cheng, Y., Sliz, P., Grigorieff, N., Harrison, S. C., Kirchhausen, T., & Walz, T. (2004) · Nature · doi:10.1038/nature03079 · Cited by 541
Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano
Horikawa, K., Yamada, Y., Matsuda, T., Kobayashi, K., Hashimoto, M., Matsu-ura, T., Miyawaki, A., Michikawa, T., Mikoshiba, K., & Nagai, T. (2010) · Nature Methods · doi:10.1038/nmeth.1488 · Cited by 338
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Cited by
Phototoxicity in live fluorescence microscopy, and how to avoid it
Icha, J., Weber, M., Waters, J. C., & Norden, C. (2017) · BioEssays · doi:10.1002/bies.201700003 · Cited by 622
Applications of bioluminescence in biotechnology and beyond
Syed, A. J., & Anderson, J. C. (2021) · Chemical Society Reviews · doi:10.1039/d0cs01492c · Cited by 341
Acid-Tolerant Monomeric GFP from Olindias formosa
Shinoda, H., Ma, Y., Nakashima, R., Sakurai, K., Matsuda, T., & Nagai, T. (2018) · Cell Chemical Biology · doi:10.1016/j.chembiol.2017.12.005 · Cited by 93
Self-citationOxygen imaging of hypoxic pockets in the mouse cerebral cortex
Beinlich, F. R. M., Asiminas, A., Untiet, V., Bojarowska, Z., Plá, V., Sigurdsson, B., Timmel, V., Gehrig, L., Graber, M. H., Hirase, H., et al. (2024) · Science · doi:10.1126/science.adn1011 · Cited by 82
Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life
Jensen, G. C., Janis, M. K., Nguyen, H. N., David, O. W., & Zastrow, M. L. (2024) · ACS Sensors · doi:10.1021/acssensors.3c02695 · Cited by 25
Metadata
- Authors
- Suzuki, K., Kimura, T., Shinoda, H., Bai, G., Daniels, M. J., Arai, Y., Nakano, M., & Nagai, T.
- Venue
- Nature Communications
- Published
- 2016-12-14
- Volume
- 7
- Issue
- 1
- Pages
- 13718-13718
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2565319794
- Cited by
- 255
- References
- 4
- FWCI
- 8.88
- Type
- journal article
- Language
- en
- Open access
- gold
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- Suzuki, K., Kimura, T., Shinoda, H., Bai, G., Daniels, M. J., Arai, Y., Nakano, M., & Nagai, T. (2016). Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nature Communications. https://doi.org/10.1038/ncomms13718
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