Acquisition of aluminium tolerance by modification of a single gene in barley
Open accessAbstract
Originating from the Fertile Crescent in the Middle East, barley has now been cultivated widely on different soil types including acid soils, where aluminium toxicity is a major limiting factor. Here we show that the adaptation of barley to acid soils is achieved by the modification of a single gene (HvAACT1) encoding a citrate transporter. We find that the primary function of this protein is to release citrate from the root pericycle cells to the xylem to facilitate the translocation of iron from roots to shoots. However, a 1-kb insertion in the upstream of the HvAACT1 coding region occurring only in the Al-tolerant accessions, enhances its expression and alters the location of expression to the root tips. The altered HvAACT1 has an important role in detoxifying aluminium by secreting citrate to the rhizosphere. Thus, the insertion of a 1-kb sequence in the HvAACT1 upstream enables barley to adapt to acidic soils.
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Only Fujii et al. (2012) had examined promoter modification.
“Other editing tools, such as transcription activator-like effector nucleases (TALENs) or prime editing, were unrepresented for the abiotic stresses, and only Fujii et al. (2012) had examined promoter modification.”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Fujii et al. (2012)
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Barley Genes as Tools to Confer Abiotic Stress Tolerance in Crops
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“Other editing tools, such as tran‐ scription activator-like effector nucleases (TALENs) or prime editing, were unrepresented for the abiotic stresses, and only Fujii et al. (2012) had examined promoter modification.”
- Authors
- Fujii, M., Yokosho, K., Yamaji, N., Saisho, D., Yamane, M., Takahashi, H., Sato, K., Nakazono, M., & Ma, J. F.
- Affiliations
- Okayama University (JP)
- Institute of Plant Science and Resources, Okayama University (JP)
- Nagoya University (JP)
- Venue
- Nature Communications
- Published
- 2012-03-06
- Volume
- 3
- Issue
- 1
- Pages
- 713-713
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC3316887
- OpenAlex
- W2054792765
- Cited by
- 197
- References
- 38
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 11 · 2023: 12 · 2022: 14 · 2021: 18 · 2020: 15 · 2019: 8 · 2018: 15 · 2017: 15 · 2016: 19 · 2015: 12 · 2014: 13 · 2013: 20 · 2012: 7
- FWCI
- 18.96
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (22119002)
- Ministry of Agriculture, Forestry and Fisheries
- Japan Society for the Promotion of Science (23658008, 21248009)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Aluminum toxicity and tolerance in plants and animals
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Keywords
- aluminum tolerance, barley, citrate transporter, acid soils, citrate secretion, iron transport, gene expression regulation, crop adaptation
- MeSH terms
- Adaptation, Physiological, Aluminum, Hordeum, Base Sequence, Biological Transport, Carrier Proteins, Hydrogen-Ion Concentration, Iron, Molecular Sequence Data, Soil, Mutagenesis, Insertional, Genes, Plant, Sequence Analysis, DNA, Gene Expression Regulation, Plant, Plant Roots, Citric Acid, Plants, Genetically Modified, Xylem, Rhizosphere
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