The barley MATE gene, HvAACT1, increases citrate efflux and Al3+ tolerance when expressed in wheat and barley
Open accessAbstract
BACKGROUND AND AIMS: Aluminium is toxic in acid soils because the soluble Al(3+) inhibits root growth. A mechanism of Al(3+) tolerance discovered in many plant species involves the release of organic anions from root apices. The Al(3+)-activated release of citrate from the root apices of Al(3+)-tolerant genotypes of barley is controlled by a MATE gene named HvAACT1 that encodes a citrate transport protein located on the plasma membrane. The aim of this study was to investigate whether expressing HvAACT1 with a constitutive promoter in barley and wheat can increase citrate efflux and Al(3+) tolerance of these important cereal species. METHODS: HvAACT1 was over-expressed in wheat (Triticum aestivum) and barley (Hordeum vulgare) using the maize ubiquitin promoter. Root apices of transgenic and control lines were analysed for HvAACT1 expression and organic acid efflux. The Al(3+) tolerance of transgenic and control lines was assessed in both hydroponic solution and acid soil. KEY RESULTS AND CONCLUSIONS: Increased HvAACT1 expression in both cereal species was associated with increased citrate efflux from root apices and enhanced Al(3+) tolerance, thus demonstrating that biotechnology can complement traditional breeding practices to increase the Al(3+) tolerance of important crop plants.
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Multiple mechanisms confer Al3+ tolerance in barley.
“Zhou et al. (2013) showed that although overexpression of HvAACT1 enhanced plant tolerance to aluminium (Al 3+ ), engineered lines were not as tolerant as the existing cultivar 'Dayton', leading the authors to propose multiple mechanisms conferring Al 3+ tolerance in barley (Table 1).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Zhou et al. (2013)
Overexpression of HvAACT1 enhanced plant tolerance to aluminium (Al3+).
“Zhou et al. (2013) showed that although overexpression of HvAACT1 enhanced plant tolerance to aluminium (Al 3+ ), engineered lines were not as tolerant as the existing cultivar 'Dayton', leading the authors to propose multiple mechanisms conferring Al 3+ tolerance in barley (Table 1).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Zhou et al. (2013)
Engineered lines overexpressing HvAACT1 were not as tolerant as the existing cultivar 'Dayton'.
“Zhou et al. (2013) showed that although overexpression of HvAACT1 enhanced plant tolerance to aluminium (Al 3+ ), engineered lines were not as tolerant as the existing cultivar 'Dayton', leading the authors to propose multiple mechanisms conferring Al 3+ tolerance in barley (Table 1).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Zhou et al. (2013)
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Barley Genes as Tools to Confer Abiotic Stress Tolerance in Crops
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“Zhou et al. (2013) showed that although overexpression of HvAACT1 enhanced plant tolerance to aluminium (Al 3+ ), engineered lines were not as tolerant as the existing cultivar ‘Dayton’, leading the authors to propose mul‐ tiple mechanisms conferring Al 3+ tolerance in barley (Table 1).”
- Authors
- Zhou, G., Delhaize, E., Zhou, M., & Ryan, P. R.
- Affiliations
- University of Tasmania (AU)
- Commonwealth Scientific and Industrial Research Organisation (AU)
- Corresponding author
- Zhou, Meixue
- Venue
- Annals of Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-06-24
- Volume
- 112
- Issue
- 3
- Pages
- 603-612
- ISSN
- 0305-7364 (ISSN-L)
- 1095-8290
- PMC
- PMC3718223
- OpenAlex
- W2129425352
- Cited by
- 127
- References
- 62
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 9 · 2023: 7 · 2022: 10 · 2021: 19 · 2020: 5 · 2019: 7 · 2018: 12 · 2017: 9 · 2016: 13 · 2015: 13 · 2014: 6 · 2013: 1
- FWCI
- 13.26
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Annals of Botany (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- eCite Digital Repository (University of Tasmania) (submittedVersion)
- Figshare (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Aluminum toxicity and tolerance in plants and animals
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Keywords
- MATE transporters, aluminum tolerance, citrate efflux, aluminum toxicity, acid soils, transgenic wheat, transgenic barley, root tip
- MeSH terms
- Aluminum, Hordeum, Carrier Proteins, Plant Proteins, Triticum, Citric Acid, Plants, Genetically Modified
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