Cited by 148Microbial Community Ecology and PhysiologyMethane Hydrates and Related PhenomenaGenomics and Phylogenetic Studies

Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface

Open access
Probst, Alexander J., Weinmaier, Thomas, Raymann, Kasie, Perras, Alexandra, Emerson, Joanne B., Rattei, Thomas, Wanner, Gerhard, Klingl, Andreas +14 more·2014·Nature Communications

Abstract

Subsurface microbial life contributes significantly to biogeochemical cycling, yet it remains largely uncharacterized, especially its archaeal members. This 'microbial dark matter' has been explored by recent studies that were, however, mostly based on DNA sequence information only. Here, we use diverse techniques including ultrastuctural analyses to link genomics to biology for the SM1 Euryarchaeon lineage, an uncultivated group of subsurface archaea. Phylogenomic analyses reveal this lineage to belong to a widespread group of archaea that we propose to classify as a new euryarchaeal order ('Candidatus Altiarchaeales'). The representative, double-membraned species 'Candidatus Altiarchaeum hamiconexum' has an autotrophic metabolism that uses a not-yet-reported Factor420-free reductive acetyl-CoA pathway, confirmed by stable carbon isotopic measurements of archaeal lipids. Our results indicate that this lineage has evolved specific metabolic and structural features like nano-grappling hooks empowering this widely distributed archaeon to predominate anaerobic groundwater, where it may represent an important carbon dioxide sink.

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Crossref · 10.1038/ncomms6497

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  • The Altiarchaea do encode the WLP.

    “This is consistent with the notion that the Altiarchaea, which may in fact belong to the DPANN archaea (Dombrowski et al. 2019), do encode the WLP (Probst et al. 2014).”

    From The Emergence of Life (2019) · cites this paper as Probst et al. 2014

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Authors
Probst, A. J., Weinmaier, T., Raymann, K., Perras, A., Emerson, J. B., Rattei, T., Wanner, G., Klingl, A., Berg, I. A., Yoshinaga, M., Viehweger, B., Hinrichs, K.-U., Thomas, B. C., Meck, S., Auerbach, A. K., Heise, M., Schintlmeister, A., Schmid, M., Wagner, M., Gribaldo, S., Banfield, J. F., & Moissl-Eichinger, C.
Affiliations
  • University of Regensburg (DE)
  • University of Vienna (AT)
  • Institut Pasteur (FR)
  • Medical University of Graz (AT)
  • University of California, Berkeley (US)
  • Ludwig-Maximilians-Universität München (DE)
  • Loewe Center for Synthetic Microbiology (DE)
  • University of Freiburg (DE)
  • University of Bremen (DE)
Corresponding author
Banfield, Jillian F.
Venue
Nature Communications
Published
2014-11-26
Volume
5
Issue
1
Pages
5497-5497
ISSN
2041-1723 (ISSN-L)
OpenAlex
W1975260887
Cited by
135
References
62
Citations by year
2026: 6 · 2025: 11 · 2024: 10 · 2023: 9 · 2022: 14 · 2021: 18 · 2020: 14 · 2019: 12 · 2018: 15 · 2017: 11 · 2016: 8 · 2015: 7
FWCI
7.53
Type
journal article
Language
en
Open access
Locations
  • Nature Communications (publishedVersion)
  • PubMed (publishedVersion)
  • University of Regensburg Publication Server (University of Regensburg) (acceptedVersion)
  • HAL (submittedVersion, other-oa)
  • RePEc: Research Papers in Economics (submittedVersion)
  • Open access LMU (Ludwid Maxmilian's Universitat Munchen) (submittedVersion)
Funders
  • U.S. Department of Energy
  • Deutsche Forschungsgemeinschaft (BE 4822/3-1)
  • Gottfried Wilhelm Leibniz Universität Hannover
  • Studienstiftung des Deutschen Volkes
  • Leibniz-Gemeinschaft
Topics
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
  • Physical Sciences › Environmental Science › Environmental Chemistry › Methane Hydrates and Related Phenomena
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
uncultivated archaea, microbial dark matter, phylogenomics, reductive acetyl-CoA pathway, autotrophic metabolism, CO2 fixation, anaerobic groundwater, carbon dioxide sink
MeSH terms
Archaea, Carbon, Molecular Sequence Data, Phylogeny, Carbon Cycle, Groundwater

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