Biology of a widespread uncultivated archaeon that contributes to carbon fixation in the subsurface
Open accessAbstract
Subsurface microbial life contributes significantly to biogeochemical cycling, yet it remains largely uncharacterized, especially its archaeal members. This 'microbial dark matter' has been explored by recent studies that were, however, mostly based on DNA sequence information only. Here, we use diverse techniques including ultrastuctural analyses to link genomics to biology for the SM1 Euryarchaeon lineage, an uncultivated group of subsurface archaea. Phylogenomic analyses reveal this lineage to belong to a widespread group of archaea that we propose to classify as a new euryarchaeal order ('Candidatus Altiarchaeales'). The representative, double-membraned species 'Candidatus Altiarchaeum hamiconexum' has an autotrophic metabolism that uses a not-yet-reported Factor420-free reductive acetyl-CoA pathway, confirmed by stable carbon isotopic measurements of archaeal lipids. Our results indicate that this lineage has evolved specific metabolic and structural features like nano-grappling hooks empowering this widely distributed archaeon to predominate anaerobic groundwater, where it may represent an important carbon dioxide sink.
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The Altiarchaea do encode the WLP.
“This is consistent with the notion that the Altiarchaea, which may in fact belong to the DPANN archaea (Dombrowski et al. 2019), do encode the WLP (Probst et al. 2014).”
From The Emergence of Life (2019) · cites this paper as Probst et al. 2014
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Self-citation
- Authors
- Probst, A. J., Weinmaier, T., Raymann, K., Perras, A., Emerson, J. B., Rattei, T., Wanner, G., Klingl, A., Berg, I. A., Yoshinaga, M., Viehweger, B., Hinrichs, K.-U., Thomas, B. C., Meck, S., Auerbach, A. K., Heise, M., Schintlmeister, A., Schmid, M., Wagner, M., Gribaldo, S., Banfield, J. F., & Moissl-Eichinger, C.
- Affiliations
- University of Regensburg (DE)
- University of Vienna (AT)
- Institut Pasteur (FR)
- Medical University of Graz (AT)
- University of California, Berkeley (US)
- Ludwig-Maximilians-Universität München (DE)
- Loewe Center for Synthetic Microbiology (DE)
- University of Freiburg (DE)
- University of Bremen (DE)
- Corresponding author
- Banfield, Jillian F.
- ORCID
- Venue
- Nature Communications
- Published
- 2014-11-26
- Volume
- 5
- Issue
- 1
- Pages
- 5497-5497
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W1975260887
- Cited by
- 135
- References
- 62
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 10 · 2023: 9 · 2022: 14 · 2021: 18 · 2020: 14 · 2019: 12 · 2018: 15 · 2017: 11 · 2016: 8 · 2015: 7
- FWCI
- 7.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion)
- PubMed (publishedVersion)
- University of Regensburg Publication Server (University of Regensburg) (acceptedVersion)
- HAL (submittedVersion, other-oa)
- RePEc: Research Papers in Economics (submittedVersion)
- Open access LMU (Ludwid Maxmilian's Universitat Munchen) (submittedVersion)
- Funders
- U.S. Department of Energy
- Deutsche Forschungsgemeinschaft (BE 4822/3-1)
- Gottfried Wilhelm Leibniz Universität Hannover
- Studienstiftung des Deutschen Volkes
- Leibniz-Gemeinschaft
- Topics
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Physical Sciences › Environmental Science › Environmental Chemistry › Methane Hydrates and Related Phenomena
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- uncultivated archaea, microbial dark matter, phylogenomics, reductive acetyl-CoA pathway, autotrophic metabolism, CO2 fixation, anaerobic groundwater, carbon dioxide sink
- MeSH terms
- Archaea, Carbon, Molecular Sequence Data, Phylogeny, Carbon Cycle, Groundwater
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