Evolutionary history of carbon monoxide dehydrogenase/acetyl-CoA synthase, one of the oldest enzymatic complexes
Open accessAbstract
Significance: Before the emergence of oxygenic photosynthesis and the accumulation of oxygen on Earth, life was essentially composed of anaerobic microorganisms. However, very little is known about which metabolisms were present at the time. Anaerobic carbon fixation through the Wood–Ljungdahl pathway is believed to be among the most ancient, and still plays a pivotal role in modern ecosystems. However, its origin and evolutionary history has been disputed. We analysed the distribution and phylogeny of carbon monoxide dehydrogenase/acetyl-CoA synthase, the main enzymatic complex of the pathway in thousands of bacterial and archaeal genomes. We show that this complex was already at work in the last universal common ancestor and has been remarkably conserved in microorganisms over more than 3.5 billion years.
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Anaerobic CO consumption involves water as a substrate and catalysts such as iron sulfide that were probably widespread.
“The Last Universal Common Ancestor was likely capable of anaerobic CO consumption (189), which involves water as a substrate and catalysts such as iron sulfide that were probably widespread”
From The Archean atmosphere (2020) · cites this paper as [189]
The Significance statement says CODH/ACS was at work in the last universal common ancestor, and the discussion identifies CO oxidation as a reaction its CdhA subunit can catalyze. The cited paper does not state that this consumption uses water as a substrate or that iron sulfide catalysts were probably widespread; its mention of an iron–sulfur complex does not establish those claims. (LLM, conf=0.86)
Experimental — accuracy not yet measuredThe Last Universal Common Ancestor was likely capable of anaerobic CO consumption.
“The Last Universal Common Ancestor was likely capable of anaerobic CO consumption (189), which involves water as a substrate and catalysts such as iron sulfide that were probably widespread”
From The Archean atmosphere (2020) · cites this paper as [189]
The Abstract infers that LUCA had a CODH/ACS complex, and the Significance section says the complex was already at work in LUCA. But the Results and Discussion leave its ancestral function unresolved; the paper does not establish that LUCA consumed CO anaerobically. (LLM, conf=0.82)
Experimental — accuracy not yet measuredACS and CODH were previously known to be homologous across the bacterial-archaeal divide based on sequence similarity.
“which were previously known to be homologous across the bacterial–archaeal divide based on sequence similarity [47, 114].”
From Evidence for corrin biosynthesis in the last universal common ancestor (2025) · cites this paper as [114]
The carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) may represent one of the oldest enzymatic complexes.
“In line with this, the reconstruction of the evolutionary history of the key enzyme of the WLP, the carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS), has led to the suggestion that it may represent one of the oldest enzymatic complexes (Adam et al. 2018).”
From The Emergence of Life (2019) · cites this paper as Adam et al. 2018
The potentially deep branching CPR bacteria and DPANN archaea do not encode the WLP.
“While it may be noteworthy that the potentially deep branching CPR bacteria and DPANN archaea do not encode the WLP (Spang et al. 2017;Adam et al. 2018), the placement of these lineages remains to be verified.”
From The Emergence of Life (2019) · cites this paper as Adam et al. 2018
The Results and Discussion describe the distribution of CODH/ACS across bacterial and archaeal lineages, but the passages found do not address whether CPR bacteria or DPANN archaea encode the Wood–Ljungdahl pathway. Searches for those lineage names and for deep-branching lineages lacking CODH/ACS found no passage establishing the attributed absence. (LLM, conf=0.72)
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“4), which were previously known to be homologous across the bacterial – archaeal divide based on sequence similarity [47,114].”
- Authors
- Adam, P. S., Borrel, G., & Gribaldo, S.
- Affiliations
- Institut Pasteur (FR)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2018-01-22
- Volume
- 115
- Issue
- 6
- Pages
- E1166-E1173
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC5819426
- OpenAlex
- W2791594771
- Cited by
- 226
- References
- 61
- Citations by year
- 2026: 27 · 2025: 20 · 2024: 32 · 2023: 39 · 2022: 28 · 2021: 21 · 2020: 21 · 2019: 24 · 2018: 14
- FWCI
- 4.62
- Type
- journal article
- Language
- en
- Update notices
- correction (2018-06-11)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Agence Nationale de la Recherche (ANR-16-CE02-0005-01)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Keywords
- Wood-Ljungdahl pathway, phylogenomics, methanogenic archaea, acetogenic bacteria, horizontal gene transfer, last universal common ancestor
- MeSH terms
- Acetate-CoA Ligase, Aldehyde Oxidoreductases, Archaea, Bacteria, Carbon Monoxide, Biological Evolution, Multienzyme Complexes, Phylogeny, Genome, Bacterial, Genome, Archaeal, Carbon Cycle
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