The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Single-copy insertion of transgenes in Caenorhabditis elegans
Open accessAbstract
At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA. These transgenes are typically overexpressed in somatic cells and silenced in the germline. We have developed a method that inserts a single copy of a transgene into a defined site. Mobilization of a Mos1 transposon generates a double-strand break in noncoding DNA. The break is repaired by copying DNA from an extrachromosomal template into the chromosomal site. Homozygous single-copy insertions can be obtained in less than 2 weeks by injecting approximately 20 worms. We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines.
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Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
piRNAs Can Trigger a Multigenerational Epigenetic Memory in the Germline of C. elegans
Ashe, A., Sapetschnig, A., Weick, E.-M., Mitchell, J., Bagijn, M. P., Cording, A. C., Doebley, A.-L., Goldstein, L. D., Lehrbach, N. J., Le Pen, J., et al. (2012) · Cell · doi:10.1016/j.cell.2012.06.018 · Cited by 754
Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery
Towbin, B. D., González-Aguilera, C., Sack, R., Gaidatzis, D., Kalck, V., Meister, P., Askjaer, P., & Gasser, S. M. (2012) · Cell · doi:10.1016/j.cell.2012.06.051 · Cited by 597
Serotonin and the neuropeptide PDF initiate and extend opposing behavioral states in C. elegans
Flavell, S. W., Pokala, N., Macosko, E. Z., Albrecht, D. R., Larsch, J., & Bargmann, C. I. (2013) · Cell · doi:10.1016/j.cell.2013.08.001 · Cited by 467
MEC-17 is an α-tubulin acetyltransferase
Akella, J. S., Wloga, D., Kim, J., Starostina, N. G., Lyons-Abbott, S., Morrissette, N. S., Dougan, S. T., Kipreos, E. T., & Gaertig, J. (2010) · Nature · doi:10.1038/nature09324 · Cited by 489
Metadata
- Authors
- Frøkjær-Jensen, C., Wayne Davis, M., Hopkins, C. E., Newman, B. J., Thummel, J. M., Olesen, S.-P., Grunnet, M., & Jorgensen, E. M.
- Venue
- Nature Genetics
- Published
- 2008-10-26
- Volume
- 40
- Issue
- 11
- Pages
- 1375-1383
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W2079458003
- Cited by
- 1,163
- References
- 27
- FWCI
- 9.80
- Type
- journal article
- Language
- en
- Open access
- green
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- Frøkjær-Jensen, C., Wayne Davis, M., Hopkins, C. E., Newman, B. J., Thummel, J. M., Olesen, S.-P., Grunnet, M., & Jorgensen, E. M. (2008). Single-copy insertion of transgenes in Caenorhabditis elegans. Nature Genetics. https://doi.org/10.1038/ng.248
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