The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Single-copy insertion of transgenes in Caenorhabditis elegans
Open accessAbstract
At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA. These transgenes are typically overexpressed in somatic cells and silenced in the germline. We have developed a method that inserts a single copy of a transgene into a defined site. Mobilization of a Mos1 transposon generates a double-strand break in noncoding DNA. The break is repaired by copying DNA from an extrachromosomal template into the chromosomal site. Homozygous single-copy insertions can be obtained in less than 2 weeks by injecting approximately 20 worms. We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines.
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Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery
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piRNAs Initiate an Epigenetic Memory of Nonself RNA in the C. elegans Germline
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Metadata
- Authors
- Frøkjær-Jensen, C., Wayne Davis, M., Hopkins, C. E., Newman, B. J., Thummel, J. M., Olesen, S.-P., Grunnet, M., & Jorgensen, E. M.
- Venue
- Nature Genetics
- Published
- 2008-10-26
- Volume
- 40
- Issue
- 11
- Pages
- 1375-1383
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W2079458003
- Cited by
- 1,163
- References
- 27
- FWCI
- 9.80
- Type
- journal article
- Language
- en
- Open access
- green
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