The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Tandem array–based expression screens identify host mRNA targets of virus-encoded microRNAs
Open accessAbstract
MicroRNAs (miRNAs) are short noncoding RNAs of cellular and viral origin that post-transcriptionally regulate gene expression through imperfect base pairing to their mRNA targets. Because the recognition sequences of miRNAs for their targets are short and may be discontinuous, bioinformatic prediction of targets is difficult. Here we present an approach to the experimental identification of the mRNA targets of miRNAs encoded by the Kaposi's sarcoma–associated herpesvirus (KSHV). KSHV encodes 17 miRNAs, derived from 12 pre-miRNAs expressed from a single locus during viral latency. We conducted multiple screens that examine small changes in transcript abundance under different conditions of miRNA expression or inhibition and then searched the identified transcripts for seed sequence matches. Using this strategy, we identified BCLAF1, encoding Bcl2-associated factor, as a target for miR-K5, and further analysis revealed that several other KSHV miRNAs also target this gene product. Our results support that this type of expression profiling provides a potentially general approach to the identification of miRNA targets.
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Cites
Silencing of microRNAs in vivo with ‘antagomirs’
Krützfeldt, J., Rajewsky, N., Braich, R., Rajeev, K. G., Tuschl, T., Manoharan, M., & Stoffel, M. (2005) · Nature · doi:10.1038/nature04303 · Cited by 3,949
A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing
Landgraf, P., Rusu, M., Sheridan, R., Sewer, A., Iovino, N., Aravin, A., Pfeffer, S., Rice, A., Kamphorst, A. O., Landthaler, M., et al. (2007) · Cell · doi:10.1016/j.cell.2007.04.040 · Cited by 3,626
The impact of microRNAs on protein output
Baek, D., Villén, J., Shin, C., Camargo, F. D., Gygi, S. P., & Bartel, D. P. (2008) · Nature · doi:10.1038/nature07242 · Cited by 3,592
Widespread changes in protein synthesis induced by microRNAs
Selbach, M., Schwanhäusser, B., Thierfelder, N., Fang, Z., Khanin, R., & Rajewsky, N. (2008) · Nature · doi:10.1038/nature07228 · Cited by 3,429 · cited 1×
“Typically, the array results showed only small (less than twofold) changes in mRNA concentrations, which is consistent with previous studie”
Requirement of bic/microRNA-155 for Normal Immune Function
Rodriguez, A., Vigorito, E., Clare, S., Warren, M. V., Couttet, P., Soond, D. R., van Dongen, S., Grocock, R. J., Das, P. P., Miska, E. A., et al. (2007) · Science · doi:10.1126/science.1139253 · Cited by 1,833
Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs
Giraldez, A. J., Mishima, Y., Rihel, J., Grocock, R. J., Van Dongen, S., Inoue, K., Enright, A. J., & Schier, A. F. (2006) · Science · doi:10.1126/science.1122689 · Cited by 1,647
Sequence-specific inhibition of small RNA function
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Cited by
Practical Aspects of microRNA Target Prediction
M. Witkos, T., Koscianska, E., & J. Krzyzosiak, W. (2011) · Current Molecular Medicine · doi:10.2174/156652411794859250 · Cited by 466
Virus-Encoded microRNAs: An Overview and a Look to the Future
Kincaid, R. P., & Sullivan, C. S. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1003018 · Cited by 396
Self-citationGay, L. A., Sethuraman, S., Thomas, M., Turner, P. C., & Renne, R. (2018) · Journal of Virology · doi:10.1128/jvi.02138-17 · Cited by 57
Exploiting the Therapeutic Potential of MicroRNAs in Viral Diseases: Expectations and Limitations
Hemida, M. G., Ye, X., Thair, S., & Yang, D. (2010) · Molecular Diagnosis & Therapy · doi:10.1007/bf03256383 · Cited by 32
Metadata
- Authors
- Ziegelbauer, J. M., Sullivan, C. S., & Ganem, D.
- Venue
- Nature Genetics
- Published
- 2008-12-21
- Volume
- 41
- Issue
- 1
- Pages
- 130-134
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W2050461547
- Cited by
- 201
- References
- 7
- FWCI
- 7.18
- Type
- journal article
- Language
- en
- Open access
- green
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