The DOI matches its Crossref record.
The role of the transcriptional regulator Ptf1a in converting intestinal to pancreatic progenitors
Abstract
Pancreas development begins with the formation of buds at specific sites in the embryonic foregut endoderm. We used recombination-based lineage tracing in vivo to show that Ptf1a (also known as PTF1-p48) is expressed at these early stages in the progenitors of pancreatic ducts, exocrine and endocrine cells, rather than being an exocrine-specific gene as previously described. Moreover, inactivation of Ptf1a switches the character of pancreatic progenitors such that their progeny proliferate in and adopt the normal fates of duodenal epithelium, including its stem-cell compartment. Consistent with the proposal that Ptf1a supports the specification of precursors of all three pancreatic cell types, transgene-based expression of Pdx1, a gene essential to pancreas formation, from Ptf1a cis-regulatory sequences restores pancreas tissue to Pdx1-null mice that otherwise lack mature exocrine and endocrine cells because of an early arrest in organogenesis. These experiments provide evidence that Ptf1a expression is specifically connected to the acquisition of pancreatic fate by undifferentiated foregut endoderm.
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“Specific recombination in epithelial cells of the pancreas was confirmed by immunohistochemical stainings for tdRFP in Ptf1aCre/+ [35] x LSL-tdRFPKI/KI [55, 56] mice (Additional file 1: Fig. S3d) [56].”
From TBX3 is dynamically expressed in pancreatic organogenesis and fine-tunes regeneration (2023) · cites this paper as [35]
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Items 601–700 of 916
Cohen, A. S., Li, J., Hight, M. R., McKinley, E. T., Fu, A., Payne, A. C., Liu, Y., Zhang, D., Xie, Q., Bai, M., et al. (2020) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-20-1214 · Cited by 25
“Ptf1a plays an essential role in mammalian pancreatic development and differentiation (45).”
BackgroundVeite-Schmahl, M. J., Joesten, W. C., & Kennedy, M. (2017) · British Journal of Cancer · doi:10.1038/bjc.2017.216 · Cited by 16
“…restricted to the pancreas by placing the Cre-recombinase expression under the control of a promoter expressed specifically in the pancreas, in this case Ptf1a, originally developed by Chris Wright group (Kawaguchi et al, 2002), that is named Ptf1a-Cre (Hingorani et al, 2003; Tuveson et al, 2004).”
BackgroundHippo signaling pathway in liver and pancreas: the potential drug target for tumor therapy
Kong, D.-L., Zhao, Y.-C., Men, T., & Teng, C.-B. (2015) · Journal of drug targeting (Print) · doi:10.3109/1061186x.2014.983522 · Cited by 16
“During pancreas development, all exocrine and endocrine cells are derived from a same population of multipotent progenitors [78,79].”
BackgroundDistinct enhancers of ptf1a mediate specification and expansion of ventral pancreas in zebrafish
Pashos, E. E., Park, J. T., Leach, S. D., & Fisher, S. (2013) · Developmental Biology · doi:10.1016/j.ydbio.2013.07.011 · Cited by 13
Vincent, R. K., Treff, N., Budde, M. L., Kastenberg, Z. J., & Odorico, J. (2006) · Stem Cells and Development · doi:10.1089/scd.2006.15.953 · Cited by 16
Forced expression of PDX-1 induces insulin production in intestinal epithelia.
Koizumi, M., Nagai, K., Kida, A., Kami, K., Ito, D., Fujimoto, K., Kawaguchi, Y., Gittes, G., & Doi, R. (2006) · Surgery · doi:10.1016/j.jss.2005.11.257 · Cited by 16
Self-citationWalter, K., Tiwary, K., Trajkovic-Arsic, M., Hidalgo-Sastre, A., Dierichs, L., Liffers, S. T., Gu, J., Gout, J., Schulte, L.-A., Münch, J., et al. (2019) · Stem Cells International · doi:10.1155/2019/8475389 · Cited by 16
“Briefly, PDAC tumors were resected from Kras ;Trp53;Ptf1a;LSL-tdRFP;Slug-YFP (KP CRS) mice expressing an oncogenic Kras mutation [11], a conditional loss of Trp53 [12], an R26-LSL-tdRFP [13] a Cre recombinase under the control of a Ptf1a promoter [14], and a Slug-YFP reporter system [10].”
MethodPaganos, P., Ronchi, P., Carl, J., Mizzon, G., Martinez, P., Benvenuto, G., & Arnone, M. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.991664 · Cited by 15
“, 2008), leads to pancreatic agenesis (Ahlgren et al., 1996; Kawaguchi et al., 2002; Marty-Santos and Cleaver, 2016).”
Endocrine Pancreas Development and Dysfunction Through the Lens of Single-Cell RNA-Sequencing
Szlachcic, W. J., Ziojla, N., Kizewska, D. K., Kempa, M., & Borowiak, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.629212 · Cited by 19
“The expression of pancreatic and duodenal homeobox 1 (PDX1) (Offield et al., 1996; Burlison et al., 2008), SRYbox transcription factor 9 (SOX9) (Lynn et al., 2007), and pancreas associated transcription factor 1A (PTF1A) (Krapp et al., 1998; Kawaguchi et al., 2002; Burlison et al., 2008) marks the multipotent pancreat…”
BackgroundDiabetes Caused by Elastase-Cre-Mediated Pdx1 Inactivation in Mice
Kodama, S., Nakano, Y., Hirata, K., Furuyama, K., Horiguchi, M., Kuhara, T., Masui, T., Kawaguchi, M., Gannon, M., Wright, C., et al. (2016) · Scientific Reports · doi:10.1038/srep21211 · Cited by 15
Self-citationGreen, N., Nicholls, Z., Heath, P., Cooper-Knock, J., Corfe, B., MacNeil, S., & Bury, J. (2014) · International journal of experimental pathology (Print) · doi:10.1111/iep.12083 · Cited by 15
Nair, G. G., Vincent, R. K., & Odorico, J. S. (2013) · Stem Cells · doi:10.1002/stem.1616 · Cited by 13
“This study also suggested that PTF1a is involved in fate choice decisions between duodenum and pancreas [5].”
InfluentialElimination of Von Hippel-Lindau Function Perturbs Pancreas Endocrine Homeostasis in Mice
Puri, S., García-Núñez, A., Hebrok, M., & Cano, D. A. (2013) · PLoS ONE · doi:10.1371/journal.pone.0072213 · Cited by 12
“Cre expression in the Ptf1a-Cre line [13] is low in endocrine cells, but robust in acinar cells and a subset of ducts (Figure S2A) [14], thus providing a useful tool to inactivate Vhlh predominantly in the exocrine compartment.”
BackgroundDelaspre, F., Massumi, M., Salido, M., Soria, B., Ravassard, P., Savatier, P., & Skoudy, A. (2013) · PLoS ONE · doi:10.1371/journal.pone.0054243 · Cited by 14
“In addition, Ptf1a is a bHLH protein essential for pancreatic formation and in its absence pancreatic progenitors assume an intestinal fate [21,22].”
BackgroundYang, Y.-K., Lei, D., An, Y., Zhang, Z., Lang, Y., Tai, S., Guo, F., & Teng, C. (2012) · FEBS Letters · doi:10.1016/j.febslet.2012.01.015 · Cited by 14
“5) [1,2], and its expression is required to commit foregut endoderm to the pancreatic fate [1,3].”
BackgroundPromoting ectopic pancreatic fates: pancreas development and future diabetes therapies
Pearl, E. J., & Horb, M. E. (2008) · Clinical Genetics · doi:10.1111/j.1399-0004.2008.01081.x · Cited by 15
“Third, progenitors lacking Ptf1a expression instead become intestinal (43).”
BackgroundPancreas transcription factor 1α expression is regulated in pancreatitis
Molero, X., Adell, T., Skoudy, A., Padilla, M. A., Gómez, J. A., Chalaux, E., Malagelada, J., & Real, F. X. (2007) · European Journal of Clinical Investigation · doi:10.1111/j.1365-2362.2007.01856.x · Cited by 16
Molecular mechanisms of β-cell dysfunction and death in monogenic forms of diabetes
Caballero, L. S., Gorgogietas, V. A., Arroyo, M. N., & Igoillo‐Esteve, M. (2021) · International review of cytology · doi:10.1016/bs.ircmb.2021.02.005 · Cited by 16
Mona, B., Villarreal, J., Savage, T. K., Kollipara, R. K., Boisvert, B. E., & Johnson, J. E. (2020) · Genes & Development · doi:10.1101/gad.332577.119 · Cited by 14
“…neural development where Ptf1a is only transiently expressed, whereas there was no phenotype detected in the pancreas where Ptf1a expression is maintained 630 GENES & DEVELOPMENT throughout adulthood, and where it is required for acinar cell maintenance (Kawaguchi et al. 2002; Hoang et al. 2016).”
BackgroundResultInfluentialARID1A loss in pancreas leads to islet developmental defect and metabolic disturbance
Kuo, T.-L., Cheng, K.-H., Chen, L.-T., & Hung, W. (2022) · iScience · doi:10.1016/j.isci.2022.105881 · Cited by 14
“However, Cre activity is extremely low or undetectable in the islet cells of Ptf1a-Cre mice.(14,15) In line with the finding, all three studies showed that the expression of Arid1a is retained in the islets of the mice.”
ResultArid1a loss potentiates pancreatic β-cell regeneration through activation of EGF signaling
Celen, C., Chuang, J.-C., Shen, S., Li, L., Maggiore, G., Jia, Y., Luo, X., Moore, A., Wang, Y., Otto, J. E., et al. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.111581 · Cited by 20
Erener, S., Ellis, C. E., Ramzy, A., Glavas, M. M., O’Dwyer, S. M., Pereira, S., Wang, T., Pang, J., Bruin, J. E., Riedel, M. J., et al. (2021) · Cell Reports Medicine · doi:10.1016/j.xcrm.2021.100434 · Cited by 16
Li, J., Wei, T., Zhang, J., & Liang, T. (2021) · Cancers · doi:10.3390/cancers13215296 · Cited by 14
Molecular identification of sixty-three amacrine cell types completes a mouse retinal cell atlasPreprint
Yan, W.-J., Laboulaye, M. A., Tran, N. M., Whitney, I. E., Benhar, I., & Sanes, J. (2020) · bioRxiv · doi:10.1101/2020.03.10.985770 · Cited by 14
Evidence of a developmental origin for β-cell heterogeneity using a dual lineage-tracing technology
Chen, C., Shiota, C., Agostinelli, G. M., Ridley, D., Jiang, Y., Ma, J., Prasadan, K., Xiao, X.-W., & Gittes, G. (2019) · Development · doi:10.1242/dev.164913 · Cited by 14
“In the fate determination and organogenesis of the mouse pancreas, Pdx1 (Gao et al., 2014; Kawaguchi et al., 2002; Kushner et al., 2002; Yang et al., 2011) and Ptf1a (Afelik et al.”
BackgroundResultInfluentialWei, W., Liu, B., Jiang, H., Jin, K., & Xiang, M. (2018) · Molecular Neurobiology · doi:10.1007/s12035-018-1186-6 · Cited by 15
Sano, M., Driscoll, D. R., DeJesus-Monge, W. E., Quattrochi, B., Appleman, V. A., Ou, J., Zhu, L. J., Yoshida, N., Yamazaki, S., Takayama, T., et al. (2016) · Neoplasia · doi:10.1016/j.neo.2016.11.004 · Cited by 14
“Genetically Engineered Mice The Elastase-tva, LSL-Kras, and Ptf1a-cre mouse strains have been previously described [7,46,47].”
BackgroundNukaya, D., Minami, K., Hoshikawa, R., Yokoi, N., & Seino, S. (2015) · Genes to Cells · doi:10.1111/gtc.12227 · Cited by 14
“In addition, Ptf1a, another important transcription factor for pancreas development (Kawaguchi et al. 2002; Zaret © 2015 The Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd Genes to Cells (2015) 20, 367–381”
BackgroundA Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell
Terasaki, M., Inoue, T., Murase, W., Kubota, A., Kojima, H., Kojoma, M., Ohta, T., Maeda, H., Miyashita, K., Mutoh, M., et al. (2021) · Cancer Genomics & Proteomics · doi:10.21873/cgp.20248 · Cited by 14
Animal Models of Pancreas Development, Developmental Disorders, and Disease
Lorberbaum, D. S., Docherty, F. M., & Sussel, L. (2020) · Advances in experimental medicine and biology · doi:10.1007/978-981-15-2389-2_3 · Cited by 18
The NOTCH Pathway in β-Cell Growth and Differentiation
Bar, Y., & Efrat, S. (2014) · Vitamins and hormones · doi:10.1016/b978-0-12-800174-5.00015-6 · Cited by 16
Kawai, M., Fukuda, A., Ikeda, M., Iimori, K., Mizukoshi, K., Iwane, K., Yamakawa, G., Omatsu, M., Namikawa, M., Sono, M., et al. (2025) · Journal of Clinical Investigation · doi:10.1172/jci177533 · Cited by 13
“To track the fate of the mutated pancreatic epithelial cells, we generated Ptf1a Cre ; LSL-Kras G12D ; Trp53 f/f ; Pbrm1 f/f , LSL-Rosa td–tomato ( KP –/– CPb –/– Tomato ) mice and compared them with the control Ptf1a Cre ; LSL-Kras G12D Trp53 f/f ; LSL-Rosa td–tomato ( KP –/– CTomato ) mice (Supple-mental Figure 5G).”
InfluentialDewa, K., Arimura, N., Kakegawa, W., Itoh, M., Adachi, T., Miyashita, S., Inoue, Y., Hizawa, K., Hori, K., Honjoya, N., et al. (2024) · Nature Communications · doi:10.1038/s41467-023-44579-z · Cited by 11
“Purkinje cell-speci fi c depletion of Dscam ( Pcp2 Cre -cKO ) reduced the number of vGluT2-positive CF synapses from proximal to distal areas, to a similar degree as that of Ptf1a Cre -cKO , in which Dscam was deleted in Purkinje cells and most cell types, except for the granule cells 63 (Fig.”
Self-citationCobo, I., Paliwal, S., Bodas, C., Felipe‐Abrio, I., Melià-Alomà, J., Torres-Sanchez, A., de Villarreal, J. M., Malumbres, M., Garcı́a, F., Millán, I., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-39291-x · Cited by 19
Self-citationShilleh, A. H., & Russ, H. A. (2023) · Cells · doi:10.3390/cells12050698 · Cited by 16
Models of pancreatic ductal adenocarcinoma
Dennaoui, R., Shrestha, H., & Wagner, K. (2021) · Cancer and Metastasis Reviews · doi:10.1007/s10555-021-09989-9 · Cited by 19
Sebae, G. K. E., Malatos, J., Cone, M., Rhee, S., Angelo, J. R., Mager, J., & Tremblay, K. D. (2018) · Development · doi:10.1242/dev.168658 · Cited by 13
“One reason these relationships have not been uncovered is because much of the retrospective fate mapping performed in the pancreas has relied on genetic techniques that are restricted to already specified pancreatic cells (Gu et al., 2002; Kawaguchi et al., 2002; Kopinke et al., 2011; Larsen et al., 2017; Offield et a…”
BackgroundMa, L.-X., & Saiyin, H. (2017) · PLoS ONE · doi:10.1371/journal.pone.0176844 · Cited by 13
Ebbers, L., Runge, K., & Nothwang, H. G. (2016) · Cell and Tissue Research · doi:10.1007/s00441-016-2401-2 · Cited by 15
Recent advances in stem cell research for the treatment of diabetes.
Noguchi, H. (2009) · World Journal of Stem Cells · doi:10.4252/wjsc.v1.i1.36 · Cited by 13
Generation and characterization of human insulin-releasing cell lines
Labriola, L., Peters, M. G., Krogh, K., Stigliano, I., Terra, L. F., Buchanan, C., Machado, M. C., Joffé, E. B. D. D. K., Puricelli, L., & Sogayar, M. (2009) · BMC Cell Biology · doi:10.1186/1471-2121-10-49 · Cited by 13
“The following primary antibodies were used: rabbit polyclonal anti-hInsulin (ICN, Aurora, OH), rabbit polyclonal anti-homeobox domain transcription factor Pdx1 [38] (kindly donated by Dr. C.V. Wright, Department of Cell Biology, School of Medicine, Vanderbilt University), rabbit polyclonal antihGlucagon (Zymed Laborat…”
MethodRetractedRovira, M., Jané‐Valbuena, J., Marchand, M., Savatier, P., Real, F. X., & Skoudy, A. (2007) · Cloning and Stem Cells · doi:10.1089/clo.2006.0064 · Cited by 10
“5 (Kawaguchi et al., 2002; Krappe et al., 1998), after Pdx1 (Offield et al., 1996).”
InfluentialPYY in the Expanding Pancreatic Epithelium
Liu, G., Dabernat, S., Kritzik, M. R., Kayali, A. G., Zhang, Y.-Q., & Sarvetnick, N. (2006) · Endocrine · doi:10.1385/endo:30:1:103 · Cited by 12
Bort, R., & Zaret, K. (2002) · Nature Genetics · doi:10.1038/ng0902-85 · Cited by 13
Circulating microRNAs in Diabetes Progression: Discovery, Validation, and Research Translation
Farr, R. J., Joglekar, M. V., & Hardikar, A. A. (2015) · Proceedings of the Fourth International Symposium on Polarization Phenomena in Nuclear Reactions · doi:10.1007/978-3-0348-0955-9_10 · Cited by 12
Cheung, E. C., Strathdee, D., Stevenson, D., Coomes, J., Blyth, K., & Vousden, K. H. (2024) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2416076121 · Cited by 12
Li, A. L., Sugiura, K., Nishiwaki, N., Suzuki, K., Sadeghian, D., Zhao, J., Maitra, A., Falvo, D., Chandwani, R., Pitarresi, J. R., et al. (2024) · Developmental Cell · doi:10.1016/j.devcel.2024.07.021 · Cited by 12
Deletion of Histone Methyltransferase G9a Suppresses Mutant Kras-driven Pancreatic Carcinogenesis
Kato, H., Tateishi, K., Fujiwara, H., Ijichi, H., Yamamoto, K., Nakatsuka, T., Kakiuchi, M., Sano, M., Kudo, Y., Hayakawa, Y., et al. (2020) · Cancer Genomics & Proteomics · doi:10.21873/cgp.20224 · Cited by 16
Cobo, I., Iglesias, M., Flández, M., Verbeke, C. S., del Pozo, N., Llorente, M., Lawlor, R. T., Luchini, C., Rusev, B. C., Scarpa, A., et al. (2020) · The Journal of Pathology · doi:10.1002/path.5570 · Cited by 14
Mallya, K., Haridas, D., Seshacharyulu, P., Pothuraju, R., Junker, W. M., Krishn, S., Muniyan, S., Vengoji, R., Batra, S., & Rachagani, S. (2020) · Biology Open · doi:10.1242/bio.052878 · Cited by 12
“Lineage tracing with Pdx1-Cre or Ptf1a-Cre (Kawaguchi et al., 2002) Cre-driver lines marks all pancreatic cell types, as both of these transcription factors are expressed in pancreatic multipotent cells (PMC) (Magnuson and Osipovich, 2013).”
InfluentialLoss of GATA4 causes ectopic pancreas in the stomach
Rodríguez-Seguel, E., Villamayor, L., Arroyo, N., de Andrés, M. P., Real, F., Martín, F., Cano, D., & Rojas, A. (2019) · Journal of Pathology · doi:10.1002/path.5378 · Cited by 12
“To determine whether loss of GATA4 specifically in pancreatic progenitors leads to the formation of ectopic pancreas in a wild-type stomach, we crossed Gata4 floxedmicewithPtf1a-Cremice [38].”
BackgroundHashemitabar, M., & Heidari, E. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.27736 · Cited by 12
“The PDX1 and PTF1A, are expressed with the onset of dorsal and ventral pancreatic bud formation (Kawaguchi et al., 2002; Offield et al., 1996).”
BackgroundOrchard, P., White, J. S., Thomas, P. E., Mychalowych, A., Kiseleva, A., Hensley, J., Allen, B. L., Parker, S., & Keegan, C. (2018) · bioRxiv · doi:10.1093/hmg/ddy378 · Cited by 12
“PTF1 drives initial pancreatic organogenesis as well as the specification of GABAergic neurons in the NT (13,15,16).”
BackgroundSeymour, P. A., Collin, C. A., Egeskov-Madsen, A., Jørgensen, M. C., Shimojo, H., Imayoshi, I., de Lichtenberg, K. D., Kopan, R., Kageyama, R., & Serup, P. (2018) · bioRxiv · doi:10.1101/336529 · Cited by 12
“, 2012), this lack of dysmorphogenesis in the early Mib1ΔPtf1a pancreas is consistent with Ptf1aCre activity in MPCs being mosaic, as previously reported (Kawaguchi et al., 2002; Seymour et al., 2012).”
BackgroundResultInfluentialProx1-Heterozygosis Sensitizes the Pancreas to Oncogenic Kras-Induced Neoplastic Transformation
Drosos, Y., Neale, G., Ye, J., Paul, L., Kuliyev, E., Maitra, A., Means, A., Washington, M., Rehg, J., Finkelstein, D. B., et al. (2016) · Neoplasia · doi:10.1016/j.neo.2016.02.002 · Cited by 12
“We investigated the expression of Prox1 in ADMs using immunostaining methods and pancreata of 2 different mouse models: wild-type mice injected with caerulein, and LSL-KrasG12D;Ptf1a+/cre mice (hereafter named KC mice).”
MethodInfluentialXenopus as a model system for studying pancreatic development and diabetes
Kofent, J., & Spagnoli, F. M. (2016) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2016.01.005 · Cited by 13
Kondratyeva, L. G., Vinogradova, T. V., Чернов, И. П., & Sverdlov, E. D. (2015) · Russian Journal of Genetics · doi:10.1134/s1022795415100099 · Cited by 11
Islet Cells Serve as Cells of Origin of Pancreatic Gastrin-Positive Endocrine Tumors
Bonnavion, R., Teinturier, R., Jaafar, R., Ripoche, D., Leteurtre, E., Chen, Y.-J., Rehfeld, J., Lépinasse, F., Hervieu, V., Pattou, F., et al. (2015) · Molecular and Cellular Biology · doi:10.1128/mcb.00302-15 · Cited by 12
“In order to determine if NPG cells were derived from pancreatic progenitors instead of a result of the migration of gut progenitors or gut differentiated cells, we performed lineage-tracing experiments with Ptf1a-Cre -R26 mice, since during development Ptf1a-expressing cells give rise only to pancreatic acinar, ductal…”
MethodThe septum transversum mesenchyme induces gall bladder development
Saito, Y., Kojima, T., & Takahashi, N. (2013) · Biology Open · doi:10.1242/bio.20135348 · Cited by 12
“…the gall bladder and the ventral pancreas, or between the ventral pancreas and the duodenum occurs in Sox17-, Hes1-, or Ptf1a-deficient mice (Kawaguchi et al., 2002; Spence et al., 2009; Sumazaki et al., 2004), indicating that these genes function cell-autonomously in the region-specific…”
BackgroundInfluentialDevelopment of the Pancreas and Related Structures
Lewis, B., & Mao, J. (2009) · doi:10.1002/9781119188421.ch1 · Cited by 12
In Vivo Regeneration of Insulin-Producing β-Cells
Jun, H. (2010) · Advances in experimental medicine and biology · doi:10.1007/978-90-481-3271-3_27 · Cited by 16
Development of the Endocrine and Exocrine Pancreas
Murtaugh, L., Cassiano, J., & Jean-PaulDeLa, O. (2008) · Springer eBooks · doi:10.1007/978-0-387-69252-4_8 · Cited by 12
“The strongest possible evidence for misspecification is to find affected cells adopting alternate fates, and studies of Ptf1a ( 30 ) and Hes1 ( 31 , 32 ) knockouts are among the few that irrefutably demonstrate this.”
BackgroundTransgenic models of pancreatic cancer
Lowy, A. (2003) · International Journal of Gastrointestinal Cancer · doi:10.1385/ijgc:33:1:71 · Cited by 12
“Similarly, the Ptf1a (PTF-1-p48) transcription factor has also been shown to direct development of all three pancreatic cell types and may prove useful in developing novel models ( 22 ).”
BackgroundDefinitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells
Zhang, Y., Yi, Y., Xiao, X., Hu, L., Xu, J.-Q., Zheng, D.-J., Koc, H. C., Chan, U., Meng, Y., Lu, L.-G., et al. (2023) · Communications Biology · doi:10.1038/s42003-023-04810-5 · Cited by 11
Differentiation Potential of Mesenchymal Stem Cells into Pancreatic β-Cells
Aydın, S., Sağraç, D., & Şahin, F. (2019) · Advances in experimental medicine and biology · doi:10.1007/5584_2019_476 · Cited by 14
Azzopardi, S., Pang, S., Klimstra, D., & Du, Y. (2016) · Neoplasia · doi:10.1016/j.neo.2016.08.003 · Cited by 11
“p48 is expressed slightly later than Pdx1 and is required to commit cells to a pancreatic fate [40].”
BackgroundPancreatic regeneration: basic research and gene regulation
Okita, K., Mizuguchi, T., Ota, S., Ishii, M., Nishidate, T., Ueki, T., Meguro, M., Kimura, Y., Tanimizu, N., Ichinohe, N., et al. (2015) · Surgery Today · doi:10.1007/s00595-015-1215-2 · Cited by 13
“, whether they differentiate into exocrine or endocrine cells) [46].”
BackgroundLin, C.-L. V., & Vuguin, P. M. (2012) · Hormone Research in Paediatrics · doi:10.1159/000337219 · Cited by 14
Kawaguchi, Y., Takaori, K., & Uemoto, S. (2011) · Journal of hepato-biliary-pancreatic sciences · doi:10.1007/s00534-010-0307-z · Cited by 11
“pdx-1 null mice, suggesting that ptf1a/pdx-1 double-positive cells mark pancreatic progenitors that can differentiate to all the cell types of the pancreas [12].”
BackgroundSelf-citationThe role of parathyroid hormone-related protein (PTHrP) in the pathophysiology of diabetes mellitus.
Legakis, I. (2009) · Mini-Reviews in Medical Chemistry · doi:10.2174/138955709788452766 · Cited by 11
Current methods in mouse models of pancreatic cancer.
Mazur, P., Herner, A., Neff, F., & Siveke, J. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2297-0_9 · Cited by 11
Experimental Metastasis: Modeling and Analysis
Malek, A. (2013) · Springer Netherlands · doi:10.1007/978-94-007-7835-1 · Cited by 11
“, p48/Pft-1 is a transcription factor during embryological development expressed by pancreatic precursor cells both of endocrine and exocrine lineages [25].”
BackgroundPancreatic β-cell remodeling in health and aging: Lessons from rodents and humans
Darwish, R., Alcibahy, Y., Dhawan, S., Butler, A. E., & Moin, A. S. M. (2025) · Ageing Research Reviews · doi:10.1016/j.arr.2025.102815 · Cited by 11
Shank2 identifies a subset of glycinergic neurons involved in altered nociception in an autism model
Heuvel, F. O., Alami, N. O., Aousji, O., Pogatzki-Zahn, E. M., Zahn, P. K., Wilhelm, H., Deshpande, D., Khatamsaz, E., Catanese, A., Woelfle, S., et al. (2023) · Molecular Autism · doi:10.1186/s13229-023-00552-7 · Cited by 24
“Vesicular GABA transporter (VGAT)-Cre x tdTomato-ROSA26 [25], pancreas associated transcription factor 1a (Ptf1a)Cre x tdTomato-ROSA26 [26], paired related homeobox protein-like 1 (Prrxl1)-Cre x tdTomato-ROSA26 (also known as DRG11); [27] and vesicular glutamate transporter 2 (vGluT2)-CRE x tdTomato-ROSA26 [25] double…”
BackgroundElliott, K. L., Iskusnykh, I., Chizhikov, V., & Fritzsch, B. (2023) · Neuroscience Letters · doi:10.1016/j.neulet.2023.137244 · Cited by 10
Cheon, C. K., Lee, Y. J., Yoo, S., Lee, J. H., Lee, J. E., Kim, H. J., Choi, I. J., Choi, Y., Lee, S., & Yoon, J. Y. (2020) · Journal of Pediatric Endocrinology and Metabolism · doi:10.1515/jpem-2020-0336 · Cited by 12
“The PTF1A gene encodes a basic helix-loop-helix protein of 48 kDa that is a sequencespecific DNA-binding subunit of PTF1 and is essential for pancreas formation during organogenesis [18].”
BackgroundHirata, K., Kodama, S., Nakano, Y., Minaki-Nakagawa, Y., Aoyama, Y., Sakikubo, M., Goto, T., Yoshida, M., Masui, T., Yamamoto, T., et al. (2019) · Scientific Reports · doi:10.1038/s41598-018-38062-9 · Cited by 10
Self-citationDevelopment of the pancreas in medaka, Oryzias latipes, from embryo to adult
Otsuka, T., Tsukahara, T., & Takeda, H. (2015) · Development, Growth and Differentiation · doi:10.1111/dgd.12237 · Cited by 10
“Ptf1a is known to participate not only in exocrine differentiation but also in early pancreatic specification (Krapp et al. 1998; Kawaguchi et al. 2002).”
BackgroundPax4 and Arx Represent Crucial Regulators of the Development of the Endocrine Pancreas
Courtney, M., Rabe, T. I., Collombat, P., & Mansouri, A. (2014) · doi:10.1155/2014/981569 · Cited by 10
RBP-J promotes the maturation of neuronal progenitors
Komine, O., Nagaoka, M., Hiraoka, Y., Hoshino, M., Kawaguchi, Y., Pear, W. S., & Tanaka, K. (2011) · Developmental Biology · doi:10.1016/j.ydbio.2011.03.020 · Cited by 10
“previously (Bajenaru et al., 2002; Kawaguchi et al., 2002), and these were used to drive Cre recombinase expression in the CNS of mice.”
MethodSelf-citationVincent, R. K., & Odorico, J. (2009) · Differentiation; research in biological diversity · doi:10.1016/j.diff.2009.03.006 · Cited by 10
Development of the pancreas and pancreatic cancer.
Lewis, B. (2006) · Endocrinology and metabolism clinics of North America (Print) · doi:10.1016/j.ecl.2006.02.003 · Cited by 10
An amylase/cre transgene marks the whole endoderm but the primordia of liver and ventral pancreas
Kockel, L., Strom, A., Delacour, A., Népote, V., Hagenbüchle, O., Wellauer, P. K., & Herrera, P. L. (2006) · genesis · doi:10.1002/dvg.20206 · Cited by 9
“On the other hand, these results are reminiscent of those obtained using a Ptf1a-p48-Cre tagger mouse in which, in addition to acini, a high proportion of ductal and islet cells were labeled (Kawaguchi et al., 2002).”
The Missing Link: Cre Pigs for Cancer Research
Kalla, D., Flisikowski, K., Yang, K., Sangüesa, L. B., Kurome, M., Kessler, B., Zakhartchenko, V., Wolf, E., Lickert, H., Saur, D., et al. (2021) · Frontiers in Oncology · doi:10.3389/fonc.2021.755746 · Cited by 9
Negrón-Piñeiro, L. J., Wu, Y., & Gregorio, A. D. (2020) · Genes · doi:10.3390/genes11111262 · Cited by 9
Weng, C., Lin, Y., & Cheng, K. (2019) · Journal of Clinical Medicine · doi:10.3390/jcm8091369 · Cited by 12
Mishra, A. K., Emamgholi, F., Erlangga, Z., Hartleben, B., Unger, K., Wolff, K., Teichmann, U., Kessel, M., Woller, N., Kühnel, F., et al. (2019) · Carcinogenesis · doi:10.1093/carcin/bgz108 · Cited by 9
The zinc finger transcription factor PW1/PEG3 restrains murine beta cell cycling
Sojoodi, M., Stradiot, L., Tanaka, K., Heremans, Y., Leuckx, G., Besson, V., Staels, W., de Casteele, M. V., Marazzi, G., Sassoon, D., et al. (2016) · Diabetologia · doi:10.1007/s00125-016-3954-z · Cited by 9
“2c, upper panels), and is essential for pancreas specification and function in both mice and humans [15].”
BackgroundHashemitabar, M., Allahbakhshi, E., Tabande, M. R., Orazizadeh, M., Dehbashi, F., Azandeh, S., Engali, K. A., & Nejad, D. (2015) · doi:10.4236/jbise.2015.811074 · Cited by 9
“Pdx-1 is a transcription factor that expressed in early stages of development of pancreatic progenitor cells [13].”
BackgroundUnderstanding Pancreas Development for β-Cell Repair and Replacement Therapies
Raducanu, A., & Lickert, H. (2012) · Current Diabetes Reports · doi:10.1007/s11892-012-0301-8 · Cited by 10
“Ptf1a is essential in maintaining the growth of the undifferentiated MPCs [55, 56] and later for exocrine-lineage specification [57].”
InfluentialIdentification of embryonic pancreatic genes using Xenopus DNA microarrays
Hayata, T., Blitz, I. L., Iwata, N., & Cho, K. W. Y. (2009) · Developmental Dynamics · doi:10.1002/dvdy.21868 · Cited by 7
“The homeodomain-containing transcription factors such as HLXB9, PDX1, PTF1A participate in pancreatic bud formation and growth (Ahlgren et al., 1996; Offield et al., 1996; Li et al., 1999; Harrison et al., 1999; Kim et al., 2002, reviewed in Schwitzgebel, 2001, Kawaguchi et al., 2002 ).”
BackgroundAnatomy, Physiology, and Embryology of the Pancreas
Fusco, J. C., El-Gohary, Y., & Gittes, G. (2019) · Shackelford's Surgery of the Alimentary Tract, 2 Volume Set · doi:10.1016/b978-0-323-40232-3.00090-x · Cited by 9
Goetz, J. J., Farris, C., Chowdhury, R., & Trimarchi, J. M. (2014) · International review of cytology · doi:10.1016/b978-0-12-800097-7.00007-5 · Cited by 9
“linked to cell-fate acquisition in the developing pancreas (Kawaguchi et al., 2002).”
BackgroundThe role of endothelial cells in pancreatic islet development, transplantation and culture
Geng, A., Yuan, S., Yu, Q. C., & Zeng, Y. A. (2025) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2025.1558137 · Cited by 7
van der Heijden, M. E., Brown, A. M., Kizek, D. J., & Sillitoe, R. V. (2023) · eLife · doi:10.7554/elife.91483 · Cited by 33
“…as L7 Cre ) ( Lewis et al., 2004 ), Slc32a1 fl (also known as Vgat fl ; JAX:012897) ( Tong et al., 2008 ), Ptf1a Cre (gift from Dr. Chris Wright) ( Kawaguchi et al., 2002 ), Slc17a6 fl (also known as Vglut2 fl ; JAX:012898) ( Tong et al., 2007 ), Pdx1 Cre (gift from Dr. Qingchun Tong) ( Gu et…”
Heterogeneity of Islet Cells during Embryogenesis and Differentiation
Sasaki, S., & Miyatsuka, T. (2023) · Diabetes & Metabolism Journal · doi:10.4093/dmj.2022.0324 · Cited by 8
“The expression of pancreatic and duodenal homeobox 1 (PDX1) [21,22], SRY-box transcription factor 9 (SOX9) [23,24], and pancreas associated transcription factor 1A (PTF1A) [22,25, 26] labels the multipotent common progenitors (CPs) within the pancreatic buds.”
BackgroundGoenka, V., Borkar, T., Desai, A., & Das, R. K. (2020) · Journal of Diabetes & Metabolic Disorders · doi:10.1007/s40200-020-00647-5 · Cited by 9
Pancreas and gallbladder agenesis in a newborn with semilobar holoprosencephaly, a case report
Hilbrands, R., Keymolen, K., Michotte, A., Marichal, M., Cools, F., Goossens, A., Veld, P. I. '., Schepper, J. D., Hattersley, A. T., & Heimberg, H. (2017) · BMC Medical Genetics · doi:10.1186/s12881-017-0419-2 · Cited by 12
Park, Y.-J., Koh, J., Kwon, J., Park, Y.-S., Yang, L., & Cha, S. (2017) · PLoS ONE · doi:10.1371/journal.pone.0169677 · Cited by 8
The Chromatin Modifier MSK1/2 Suppresses Endocrine Cell Fates during Mouse Pancreatic Development
Bhat, N., Park, J., Zoghbi, H., Arthur, J. S., & Zaret, K. (2016) · PLoS ONE · doi:10.1371/journal.pone.0166703 · Cited by 8
Metadata
- Authors
- Kawaguchi, Y., Cooper, B., Gannon, M., Ray, M., MacDonald, R. J., & Wright, C. V. E.
- Affiliations
- Vanderbilt University (US)
- The University of Texas Southwestern Medical Center (US)
- Corresponding author
- Wright, Christopher V.E.
- Venue
- Nature Genetics
- Published
- 2002-08-19
- Volume
- 32
- Issue
- 1
- Pages
- 128-134
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W1993055783
- Cited by
- 1,007
- References
- 26
- Citations by year
- 2026: 18 · 2025: 30 · 2024: 18 · 2023: 39 · 2022: 23 · 2021: 39 · 2020: 39 · 2019: 44 · 2018: 43 · 2017: 44 · 2016: 44 · 2015: 62 · 2014: 54 · 2013: 65 · 2012: 68
- FWCI
- 35.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
- Keywords
- Ptf1a, pancreatic development, foregut endoderm, pancreatic progenitors, duodenal epithelium, pancreatic stem cells, PDX1, cis-regulatory sequences, lineage tracing, organogenesis
- MeSH terms
- Animals, Cell Differentiation, Duodenum, Gene Expression Regulation, Pancreas, Transcription Factors, Trans-Activators, Homeodomain Proteins, Cell Lineage, Animals, Genetically Modified, Mice
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