Afferent lymph-derived T cells and DCs use different chemokine receptor CCR7-dependent routes for entry into the lymph node and intranodal migration
Open accessAbstract
Little is known about the molecular mechanisms that determine the entry into the lymph node and intranodal positioning of lymph-derived cells. By injecting cells directly into afferent lymph vessels of popliteal lymph nodes, we demonstrate that lymph-derived T cells entered lymph-node parenchyma mainly from peripheral medullary sinuses, whereas dendritic cells (DCs) transmigrated through the floor of the subcapsular sinus on the afferent side. Transmigrating DCs induced local changes that allowed the concomitant entry of T cells at these sites. Signals mediated by the chemokine receptor CCR7 were absolutely required for the directional migration of both DCs and T cells into the T cell zone but were dispensable for the parenchymal entry of lymph-derived T cells and dendrite probing of DCs. Our findings provide insight into the molecular and structural requirements for the entry into lymph nodes and intranodal migration of lymph-derived cells of the immune system.
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Larger cells such as DCs, macrophages, and activated T cells gain access to the lymph node from the SCS.
“By contrast, larger cells such as DCs, macrophages, and activated T cells gain access to the lymph node from the SCS (92–94).”
From Transport and Immune Functions of the Lymphatic System. (2025) · cites this paper as [92]
Small resting T cells pass from the SCS to medullary lymphatic sinuses and gain access to the lymph node at this site.
“From the SCS, small resting T cells pass to medullary lymphatic sinuses and gain access to the lymph node at this site (92–94).”
From Transport and Immune Functions of the Lymphatic System. (2025) · cites this paper as [92]
Transmigration of DCs and activated T cells across the SCS into the lymph node parenchyma is dependent on CCR7.
“Transmigration of DCs and activated T cells across the SCS into the lymph node parenchyma is also dependent on CCR7 (92, 94).”
From Transport and Immune Functions of the Lymphatic System. (2025) · cites this paper as [92]
Entry of DCs and activated T cells from the SCS to the lymph node appears to be independent of integrins even under inflammatory conditions.
“However, in contrast to their entry into initial LVs, entry of DCs and activated T cells from the SCS to the lymph node appears to be independent of integrins even under inflammatory conditions (92, 94).”
From Transport and Immune Functions of the Lymphatic System. (2025) · cites this paper as [92]
After entry into the lymph node, access of all immunocytes to their respective locations depends upon CCR7 and CCL21.
“After entry into the lymph node, access of all immunocytes to their respective locations depends upon CCR7 and CCL21 (92, 94).”
From Transport and Immune Functions of the Lymphatic System. (2025) · cites this paper as [92]
Entry of resting T cells from the medullary sinuses to the lymph node parenchyma is independent of CCR7.
“In contrast, entry of resting T cells from the medullary sinuses, to which they gain access based upon their small size alone, to the lymph node parenchyma is independent of CCR7 (92).”
From Transport and Immune Functions of the Lymphatic System. (2025) · cites this paper as [92]
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- Authors
- Braun, A., Worbs, T., Moschovakis, G. L., Halle, S., Hoffmann, K., Bölter, J., Münk, A., & Förster, R.
- Affiliations
- Medizinische Hochschule Hannover (DE)
- Corresponding author
- Förster, Reinhold
- Venue
- Nature Immunology
- Published
- 2011-08-14
- Volume
- 12
- Issue
- 9
- Pages
- 879-887
- ISSN
- 1529-2908 (ISSN-L)
- 1529-2916
- OpenAlex
- W2145172700
- Cited by
- 337
- References
- 0
- Citations by year
- 2026: 10 · 2025: 18 · 2024: 16 · 2023: 24 · 2022: 23 · 2021: 29 · 2020: 21 · 2019: 21 · 2018: 24 · 2017: 21 · 2016: 29 · 2015: 18 · 2014: 27 · 2013: 23 · 2012: 23
- FWCI
- 12.89
- Type
- journal article
- Language
- en
- Open access
- green
- hal.science
- Licence
- other-oa
- www.springer.com/tdm (tdm)
- Locations
- Nature Immunology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Trinity's Access to Research Output (TARA) (Trinity College Dublin) (submittedVersion)
- Funders
- Medizinischen Hochschule Hannover
- Topics
- Health Sciences › Medicine › Oncology › Lymphatic System and Diseases
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- afferent lymph, dendritic cells, T cells, CCR7, chemokine signaling, lymphatic vessels, subcapsular sinus, T cell zone, lymphocyte homing
- MeSH terms
- Animals, Dendritic Cells, Flow Cytometry, Humans, Injections, Intralymphatic, Lymph, Lymph Nodes, Mice, Inbred BALB C, CD4-Positive T-Lymphocytes, Mice, Knockout, Chemokines, CC, Lymphatic Vessels, Mice, Receptors, CCR7, Transcellular Cell Migration, Transendothelial and Transepithelial Migration
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