Cited by 222T-cell and B-cell ImmunologyGenomics and Chromatin DynamicsDevelopmental Biology and Gene Regulation

Transcription factor EBF1 is essential for the maintenance of B cell identity and prevention of alternative fates in committed cells

Nechanitzky, Robert, Akbas, Duygu, Scherer, Stefanie, Györy, Ildiko, Hoyler, Thomas, Ramamoorthy, Senthilkumar, Diefenbach, Andreas, Grosschedl, Rudolf·2013·Nature Immunology

Abstract

The transcription factors EBF1 and Pax5 have been linked to activation of the B cell lineage program and irreversible loss of alternative lineage potential (commitment), respectively. Here we conditionally deleted Ebf1 in committed pro-B cells after transfer into alymphoid mice. We found that those cells converted into innate lymphoid cells (ILCs) and T cells with variable-diversity-joining (VDJ) rearrangements of loci encoding both B cell and T cell antigen receptors. As intermediates in lineage conversion, Ebf1-deficient CD19(+) cells expressing Pax5 and transcriptional regulators of the ILC and T cell fates were detectable. In particular, genes encoding the transcription factors Id2 and TCF-1 were bound and repressed by EBF1. Thus, both EBF1 and Pax5 are required for B lineage commitment by repressing distinct and common determinants of alternative cell fates.

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Crossref · 10.1038/ni.2641

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Authors
Nechanitzky, R., Akbas, D., Scherer, S., Györy, I., Hoyler, T., Ramamoorthy, S., Diefenbach, A., & Grosschedl, R.
Affiliations
  • Max Planck Institute of Immunobiology and Epigenetics (DE)
  • Max Planck Society (DE)
  • University of Freiburg (DE)
Corresponding author
Grosschedl, Rudolf
Venue
Nature Immunology
Published
2013-06-30
Volume
14
Issue
8
Pages
867-875
ISSN
  • 1529-2908 (ISSN-L)
  • 1529-2916
OpenAlex
W2064993419
Cited by
218
References
50
Citations by year
2026: 13 · 2025: 22 · 2024: 19 · 2023: 12 · 2022: 19 · 2021: 14 · 2020: 19 · 2019: 10 · 2018: 14 · 2017: 17 · 2016: 20 · 2015: 17 · 2014: 18 · 2013: 1
FWCI
10.30
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Digital Library (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
EBF1, B cell identity, lineage commitment, pro-B cells, innate lymphoid cells, T cell fate, PAX5, Id2, TCF1, VDJ rearrangement, conditional gene deletion, transcriptional repression
MeSH terms
Animals, B-Lymphocytes, Cell Differentiation, DNA, Gene Expression Regulation, Receptors, Antigen, T-Cell, T-Lymphocytes, Trans-Activators, Polymerase Chain Reaction, Mice, Knockout, Cell Lineage, Adoptive Transfer, Oligonucleotide Array Sequence Analysis, Lymphopoiesis, Mice, V(D)J Recombination

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