The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Concerted peptide trimming by human ERAP1 and ERAP2 aminopeptidase complexes in the endoplasmic reticulum
Abstract
The generation of many HLA class I peptides entails a final trimming step in the endoplasmic reticulum that, in humans, is accomplished by two 'candidate' aminopeptidases. We show here that one of these, ERAP1, was unable to remove several N-terminal amino acids that were trimmed efficiently by the second enzyme, ERAP2. This trimming of a longer peptide required the concerted action of both ERAP1 and ERAP2, both for in vitro digestion and in vivo for cellular antigen presentation. ERAP1 and ERAP2 localized together in vivo and associated physically in complexes that were most likely heterodimeric. Thus, the human endoplasmic reticulum is equipped with a pair of trimming aminopeptidases that have complementary functions in HLA class I peptide presentation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Inspired by models and algorithms developed for detecting contamination in DNA sequencing18, demuxlet is fast, accurate, scalable, and compatible with standard input formats17, 19, 20.”
From Multiplexed droplet single-cell RNA-sequencing using natural genetic variation (2018) · cites this paper as [20]
Citations
Cites
Wessel, D., & Flügge, U. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90782-6 · Cited by 3,997
Genome sequence of the Brown Norway rat yields insights into mammalian evolution
Gibbs, R. A., Weinstock, G. M., Metzker, M. L., Muzny, D. M., Sodergren, E. J., Scherer, S. E., Scott, G., Steffen, D. L., Worley, K. C., Burch, P. E., et al. (2004) · Nature · doi:10.1038/nature02426 · Cited by 2,170
DEGRADATION OF CELL PROTEINS AND THE GENERATION OF MHC CLASS I-PRESENTED PEPTIDES
Rock, K. L., & Goldberg, A. L. (1999) · Annual Review of Immunology · doi:10.1146/annurev.immunol.17.1.739 · Cited by 923
ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum
Serwold, T. F., Gonzãlez, F., Kim, J. S., Jacob, R. J., & Shastri, N. (2002) · Nature · doi:10.1038/nature01074 · Cited by 631
A viral inhibitor of peptide transporters for antigen presentation
Früh, K., Ahn, K., Djaballah, H., Sempé, P., Endert, P. V., Tampé, R., Peterson, P. A., & Yang, Y. M. (1995) · Nature · doi:10.1038/375415a0 · Cited by 601
An IFN-γ–induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I–presented peptides
Šarić, T., Chang, S., Hattori, A., York, I. A., Markant, S. L., Rock, K. L., Tsujimoto, M., & Goldberg, A. L. (2002) · Nature Immunology · doi:10.1038/ni859 · Cited by 532
York, I. A., Chang, S., Šarić, T., Keys, J. A., Favreau, J., Goldberg, A. L., & Rock, K. L. (2002) · Nature Immunology · doi:10.1038/ni860 · Cited by 514
Current Protocols in Cell Biology
Bonifacino, J. S., Dasso, M., Harford, J. B., Lippincott‐Schwartz, J., & Yamada, K. M. (2001) · John Wiley & Sons, Inc. eBooks · doi:10.1002/0471143030 · Cited by 509
Producing Nature’s Gene-Chips: The Generation of Peptides for Display by MHC Class I Molecules
Shastri, N., Schwab, S. R., & Serwold, T. F. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.100301.064819 · Cited by 304
Endert, P. V., Tampé, R., Meyer, T. H., Tisch, R., Bach, J., & McDevitt, H. O. (1994) · Immunity · doi:10.1016/1074-7613(94)90091-4 · Cited by 287
Post-proteasomal antigen processing for major histocompatibility complex class I presentation
Rock, K. L., York, I. A., & Goldberg, A. L. (2004) · Nature Immunology · doi:10.1038/ni1089 · Cited by 280
Reits, E. A., Neijssen, J., Herberts, C. A., Benckhuijsen, W. E., Janssen, L., Drijfhout, J. W., & Neefjes, J. (2004) · Immunity · doi:10.1016/s1074-7613(04)00074-3 · Cited by 261
Identification of ARTS-1 as a novel TNFR1-binding protein that promotes TNFR1 ectodomain shedding
Cui, X., Hawari, F. I., Alsaaty, S., Lawrence, M. G., Combs, C. A., Geng, W., Rouhani, F. N., Miskinis, D., & Levine, S. J. (2002) · Journal of Clinical Investigation · doi:10.1172/jci13847 · Cited by 205
Recognition principle of the TAP transporter disclosed by combinatorial peptide libraries
Uebel, S. F. W., Kraas, W., Kienle, S., Wiesmüller, K., Jung, G., & Tampé, R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.17.8976 · Cited by 201
Human Leukocyte-derived Arginine Aminopeptidase
Tanioka, T., Hattori, A., Masuda, S., Nomura, Y., Nakayama, H., Mizutani, S., & Tsujimoto, M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305076200 · Cited by 194
Rowe, M., Khanna, R., Jacob, C., Argaet, V. P., Kelly, A. P., Powis, S. H., Belich, M. P., Groom‐Carter, D., Lee, S., Burrows, S. R., et al. (1995) · European Journal of Immunology · doi:10.1002/eji.1830250536 · Cited by 193
ER aminopeptidases generate a unique pool of peptides for MHC class I molecules
Serwold, T. F., Gaw, S. L., & Shastri, N. (2001) · Nature Immunology · doi:10.1038/89800 · Cited by 192
The peptide-binding motif for the human transporter associated with antigen processing.
Endert, P. V., Riganelli, D., Greco, G., Fleischhauer, K., Sidney, J., Sette, A., & Bach, J. (1995) · The Journal of Experimental Medicine · doi:10.1084/jem.182.6.1883 · Cited by 181
Identification of ARTS-1 as a novel TNFR1-binding protein that promotes TNFR1 ectodomain shedding
Cui, X., Hawari, F. I., Alsaaty, S., Lawrence, M. G., Combs, C. A., Geng, W., Rouhani, F. N., Miskinis, D., & Levine, S. J. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0213847 · Cited by 172
Neisig, A., Roelse, J., Sijts, A. J. A. M., OSSENDORP, F. A., Feltkamp, M. C. W., Kast, W. M., Melief, C. J. M., & Neefjes, J. (1995) · The Journal of Immunology · doi:10.4049/jimmunol.154.3.1273 · Cited by 157
York, I. A., Mo, A. X. Y., Lemerise, K., Zeng, W., Shen, Y., Abraham, C. R., Šarić, T., Goldberg, A. L., & Rock, K. L. (2003) · Immunity · doi:10.1016/s1074-7613(03)00058-x · Cited by 155
Koopmann, J., Albring, J. C., Huter, E. N., Bulbuc, N., Spee, P., Neefjes, J., Hämmerling, G. J., & Momburg, F. (2000) · Immunity · doi:10.1016/s1074-7613(00)00013-3 · Cited by 153
Roelse, J., Grommé, M., Momburg, F., Hämmerling, G. J., & Neefjes, J. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.180.5.1591 · Cited by 153
Šarić, T., Beninga, J., Graef, C. I., Akopian, T. N., Rock, K. L., & Goldberg, A. L. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m105517200 · Cited by 144
Hattori, A., Matsumoto, H., Mizutani, S., & Tsujimoto, M. (1999) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a022371 · Cited by 113
Matsumoto, H., Rogi, T., Yamashiro, K., Kodama, S., Tsuruoka, N., Hattori, A., Takio, K., Mizutani, S., & Tsujimoto, M. (2000) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2000.00949.x · Cited by 109
Hattori, A., Kitatani, K., Matsumoto, H., Miyazawa, S., Rogi, T., Tsuruoka, N., Mizutani, S., Natori, Y., & Tsujimoto, M. (2000) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a022812 · Cited by 106
Daniel, S., Brusić, V., Caillat‐Zucman, S., Petrovsky, N., Harrison, L. C., Riganelli, D., Sinigaglia, F., Gallazzi, F., Hammer, J., & Endert, P. V. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.161.2.617 · Cited by 97
Lauvau, G. S., Kakimi, K., Niedermann, G., Ostankovitch, M., Yotnda, P., Firat, H., Chisari, F. V., & Endert, P. V. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.190.9.1227 · Cited by 91
Self-citationFruci, D., Niedermann, G., Butler, R. H., & Endert, P. V. (2001) · Immunity · doi:10.1016/s1074-7613(01)00203-5 · Cited by 75
Self-citationMolecular characterization of a puromycin‐insensitive leucyl‐specific aminopeptidase, PILS‐AP
Schomburg, L., Kollmus, H., Friedrichsen, S., & Bauer, K. (2000) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2000.01348.x · Cited by 65
Self-citationBrouwenstijn, N., Serwold, T., & Shastri, N. (2001) · Immunity · doi:10.1016/s1074-7613(01)00174-1 · Cited by 61
A chaperone-assisted high yield system for the production of HLA-DR4 tetramers in insect cells
Fourneau, J., Cohen, H., & Endert, P. V. (2004) · Journal of Immunological Methods · doi:10.1016/j.jim.2003.11.011 · Cited by 30
Samino, Y., López, D., Guil, S., de León, P., & Val, M. D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m305343200 · Cited by 17
Determination of Molecular Size by Zonal Sedimentation Analysis on Sucrose Density Gradients
Marks, M. S. (1998) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0503s00 · Cited by 14
Cited by
Sorted by citations
Franke, A., McGovern, D. P. B., Barrett, J. C., Wang, K., Radford-Smith, G. L., Ahmad, T., Lees, C. W., Balschun, T., Lee, J., Roberts, R., et al. (2010) · Nature Genetics · doi:10.1038/ng.717 · Cited by 2,520
Towards a systems understanding of MHC class I and MHC class II antigen presentation
Neefjes, J., Jongsma, M. L. M., Paul, P., & Bakke, O. (2011) · Nature Reviews Immunology · doi:10.1038/nri3084 · Cited by 2,044
Pathways of Antigen Processing
Blum, J. S., Wearsch, P. A., & Cresswell, P. (2013) · Annual Review of Immunology · doi:10.1146/annurev-immunol-032712-095910 · Cited by 1,635
Association scan of 14,500 nonsynonymous SNPs in four diseases identifies autoimmunity variants
Wellcome Trust Case Control Consortium, & The Australo-Anglo-American Spondylitis Consortium (TASC) (2007) · Nature Genetics · doi:10.1038/ng.2007.17 · Cited by 1,374
Multiplexed droplet single-cell RNA-sequencing using natural genetic variation
Kang, H. M., Subramaniam, M., Targ, S., Nguyen, M., Maliskova, L., McCarthy, E., Wan, E., Wong, S., Byrnes, L., Lanata, C. M., et al. (2018) · Nature Biotechnology · doi:10.1038/nbt.4042 · Cited by 1,101
“…those in the major histocompatibility complex (MHC) class I antigen presentation pathway including ERAP2 (P 3.57×10 −32 , meta-analysis), encoding an aminopeptidase known to cleave viral peptides 34 , and HLA-C (P 1.74×10 −29 , meta-analysis), which encodes the MHC class I heavy chain (Fig.”
BackgroundInfluential
Metadata
- Authors
- Saveanu, L., Carroll, O., Lindo, V., Del Val, M., Lopez, D., Lepelletier, Y., Greer, F., Schomburg, L., Fruci, D., Niedermann, G., & van Endert, P. M.
- Affiliations
- Inserm (FR)
- Centre National de la Recherche Scientifique (FR)
- Charité – Universitätsmedizin Berlin (DE)
- Max Planck Society (DE)
- Venue
- Nature Immunology
- Published
- 2005-05-22
- Volume
- 6
- Issue
- 7
- Pages
- 689-697
- ISSN
- 1529-2908 (ISSN-L)
- 1529-2916
- OpenAlex
- W1981338942
- Cited by
- 481
- References
- 35
- Citations by year
- 2026: 8 · 2025: 18 · 2024: 18 · 2023: 31 · 2022: 20 · 2021: 23 · 2020: 28 · 2019: 30 · 2018: 25 · 2017: 25 · 2016: 15 · 2015: 26 · 2014: 25 · 2013: 31 · 2012: 27
- FWCI
- 7.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Immunology (publishedVersion)
- PubMed (publishedVersion)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Funders
- Comunidad de Madrid
- European Commission
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Peptidase Inhibition and Analysis
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- ERAP1, ERAP2, aminopeptidase, peptide trimming, HLA class I, endoplasmic reticulum, antigen presentation
- MeSH terms
- Aminopeptidases, Epitopes, Endoplasmic Reticulum, HeLa Cells, Humans, Microsomes, Peptide Fragments, RNA, Immunoblotting, Histocompatibility Antigens Class I, Minor Histocompatibility Antigens, Antigen Presentation, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
