The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Concerted peptide trimming by human ERAP1 and ERAP2 aminopeptidase complexes in the endoplasmic reticulum
Abstract
The generation of many HLA class I peptides entails a final trimming step in the endoplasmic reticulum that, in humans, is accomplished by two 'candidate' aminopeptidases. We show here that one of these, ERAP1, was unable to remove several N-terminal amino acids that were trimmed efficiently by the second enzyme, ERAP2. This trimming of a longer peptide required the concerted action of both ERAP1 and ERAP2, both for in vitro digestion and in vivo for cellular antigen presentation. ERAP1 and ERAP2 localized together in vivo and associated physically in complexes that were most likely heterodimeric. Thus, the human endoplasmic reticulum is equipped with a pair of trimming aminopeptidases that have complementary functions in HLA class I peptide presentation.
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“Inspired by models and algorithms developed for detecting contamination in DNA sequencing18, demuxlet is fast, accurate, scalable, and compatible with standard input formats17, 19, 20.”
From Multiplexed droplet single-cell RNA-sequencing using natural genetic variation (2018) · cites this paper as [20]
Citations
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ERAAP customizes peptides for MHC class I molecules in the endoplasmic reticulum
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An IFN-γ–induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I–presented peptides
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“…those in the major histocompatibility complex (MHC) class I antigen presentation pathway including ERAP2 (P 3.57×10 −32 , meta-analysis), encoding an aminopeptidase known to cleave viral peptides 34 , and HLA-C (P 1.74×10 −29 , meta-analysis), which encodes the MHC class I heavy chain (Fig.”
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Metadata
- Authors
- Saveanu, L., Carroll, O., Lindo, V., Del Val, M., Lopez, D., Lepelletier, Y., Greer, F., Schomburg, L., Fruci, D., Niedermann, G., & van Endert, P. M.
- Affiliations
- Inserm (FR)
- Centre National de la Recherche Scientifique (FR)
- Charité – Universitätsmedizin Berlin (DE)
- Max Planck Society (DE)
- Venue
- Nature Immunology
- Published
- 2005-05-22
- Volume
- 6
- Issue
- 7
- Pages
- 689-697
- ISSN
- 1529-2908 (ISSN-L)
- 1529-2916
- OpenAlex
- W1981338942
- Cited by
- 481
- References
- 35
- Citations by year
- 2026: 8 · 2025: 18 · 2024: 18 · 2023: 31 · 2022: 20 · 2021: 23 · 2020: 28 · 2019: 30 · 2018: 25 · 2017: 25 · 2016: 15 · 2015: 26 · 2014: 25 · 2013: 31 · 2012: 27
- FWCI
- 7.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Immunology (publishedVersion)
- PubMed (publishedVersion)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Funders
- Comunidad de Madrid
- European Commission
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Peptidase Inhibition and Analysis
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- ERAP1, ERAP2, aminopeptidase, peptide trimming, HLA class I, endoplasmic reticulum, antigen presentation
- MeSH terms
- Aminopeptidases, Epitopes, Endoplasmic Reticulum, HeLa Cells, Humans, Microsomes, Peptide Fragments, RNA, Immunoblotting, Histocompatibility Antigens Class I, Minor Histocompatibility Antigens, Antigen Presentation, Reverse Transcriptase Polymerase Chain Reaction, RNA Interference
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