The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Conversion of vascular endothelial cells into multipotent stem-like cells
Open accessAbstract
Mesenchymal stem cells can give rise to several cell types, but varying results depending on isolation methods and tissue source have led to controversies about their usefulness in clinical medicine. Here we show that vascular endothelial cells can transform into multipotent stem-like cells by an activin-like kinase-2 (ALK2) receptor–dependent mechanism. In lesions from individuals with fibrodysplasia ossificans progressiva (FOP), a disease in which heterotopic ossification occurs as a result of activating ALK2 mutations, or from transgenic mice expressing constitutively active ALK2, chondrocytes and osteoblasts expressed endothelial markers. Lineage tracing of heterotopic ossification in mice using a Tie2-Cre construct also suggested an endothelial origin of these cell types. Expression of constitutively active ALK2 in endothelial cells caused endothelial-to-mesenchymal transition and acquisition of a stem cell–like phenotype. Similar results were obtained by treatment of untransfected endothelial cells with the ligands transforming growth factor-β2 (TGF-β2) or bone morphogenetic protein-4 (BMP4) in an ALK2-dependent manner. These stem-like cells could be triggered to differentiate into osteoblasts, chondrocytes or adipocytes. We suggest that conversion of endothelial cells to stem-like cells may provide a new approach to tissue engineering.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The basics of epithelial-mesenchymal transition
Kalluri, R., & Weinberg, R. A. (2009) · Journal of Clinical Investigation · doi:10.1172/jci39104 · Cited by 10,196
Self-citationEpithelial–mesenchymal transitions in tumour progression
Thiery, J. P. (2002) · Nature Reviews Cancer · doi:10.1038/nrc822 · Cited by 6,628
Chamberlain, G., Fox, J., Ashton, B., & Middleton, J. (2007) · Stem Cells · doi:10.1634/stemcells.2007-0197 · Cited by 2,381
Endothelial-to-mesenchymal transition contributes to cardiac fibrosis
Zeisberg, E. M., Tarnavski, O., Zeisberg, M., Dorfman, A. L., McMullen, J. R., Gustafsson, E., Chandraker, A., Yuan, X., Pu, W. T., Roberts, A. B., et al. (2007) · Nature Medicine · doi:10.1038/nm1613 · Cited by 2,222
Self-citationChen, D., Zhao, M., & Mundy, G. R. (2004) · Growth Factors · doi:10.1080/08977190412331279890 · Cited by 1,915
Epithelial–mesenchymal transitions in development and pathologies
Thiery, J. P. (2003) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2003.10.006 · Cited by 1,704
Shore, E. M., Xu, M., Feldman, G. J., Fenstermacher, D. A., Cho, T.-J., Choi, I. H., Connor, J. M., Delai, P., Glaser, D. L., LeMerrer, M., et al. (2006) · Nature Genetics · doi:10.1038/ng1783 · Cited by 1,215
Self-citationDiscovery of Endothelial to Mesenchymal Transition as a Source for Carcinoma-Associated Fibroblasts
Zeisberg, E. M., Potenta, S., Xie, L., Zeisberg, M., & Kalluri, R. (2007) · Cancer Research · doi:10.1158/0008-5472.can-07-3127 · Cited by 1,011
Self-citationFibroblasts in Kidney Fibrosis Emerge via Endothelial-to-Mesenchymal Transition
Zeisberg, E. M., Potenta, S. E., Sugimoto, H., Zeisberg, M., & Kalluri, R. (2008) · Journal of the American Society of Nephrology · doi:10.1681/asn.2008050513 · Cited by 876
Self-citationThe mesenchymal cell, its role in the embryo, and the remarkable signaling mechanisms that create it
Hay, E. D. (2005) · Developmental Dynamics · doi:10.1002/dvdy.20345 · Cited by 641
The role of endothelial-to-mesenchymal transition in cancer progression
Potenta, S., Zeisberg, E., & Kalluri, R. (2008) · British Journal of Cancer · doi:10.1038/sj.bjc.6604662 · Cited by 554
Self-citationThe principles of the Ilizarov method.
Ilizarov, G. A. (1988) · PubMed · Cited by 432
VEGF: an Essential Mediator of Both Angiogenesis and Endochondral Ossification
Dai, J., & Rabie, A. B. M. (2007) · Journal of Dental Research · doi:10.1177/154405910708601006 · Cited by 404
Jinnin, M., Medici, D., Park, L., Limaye, N., Liu, Y., Boscolo, E., Bischoff, J., Vikkula, M., Boye, E., & Olsen, B. R. (2008) · Nature Medicine · doi:10.1038/nm.1877 · Cited by 370
Self-citationArciniegas, E., Frid, M. G., Douglas, I. S., & Stenmark, K. R. (2007) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00378.2006 · Cited by 341
Li, J., Qu, X., & Bertram, J. F. (2009) · The American Journal of Pathology · doi:10.2353/ajpath.2009.090096 · Cited by 316
Amarilio, R., Viukov, S. V., Sharir, A., Eshkar-Oren, I., Johnson, R. S., & Zelzer, E. (2007) · Development · doi:10.1242/dev.008441 · Cited by 313
Identification of Progenitor Cells That Contribute to Heterotopic Skeletogenesis
Lounev, V. Y., Ramachandran, R., Wosczyna, M. N., Yamamoto, M., Maidment, A. D., Shore, E. M., Glaser, D. L., Goldhamer, D. J., & Kaplan, F. S. (2009) · The Journal of Bone and Joint Surgery-American Volume · doi:10.2106/jbjs.h.01177 · Cited by 303
Self-citationClonality and altered behavior of endothelial cells from hemangiomas
Boye, E., Yu, Y., Paranya, G., Mulliken, J. B., Olsen, B. R., & Bischoff, J. (2001) · Journal of Clinical Investigation · doi:10.1172/jci11432 · Cited by 298
Self-citationCamenisch, T. D., Molin, D. G. M., Person, A., Runyan, R. B., Gittenberger-de Groot, A. C., McDonald, J. A., & Klewer, S. E. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0731 · Cited by 279
Boyer, A. S., Ayerinskas, I. I., Vincent, E. B., McKinney, L. A., Weeks, D. L., & Runyan, R. B. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9211 · Cited by 204
Inflammatory Hypoxia: Role of Hypoxia-Inducible Factor
Karhausen, J., Haase, V. H., & Colgan, S. P. (2005) · Cell Cycle · doi:10.4161/cc.4.2.1407 · Cited by 164
Paruchuri, S. M., Yang, J.-H., Aïkawa, E., Melero‐Martin, J. M., Khan, Z. A., Loukogeorgakis, S., Schöen, F. J., & Bischoff, J. E. (2006) · Circulation Research · doi:10.1161/01.res.0000245188.41002.2c · Cited by 161
Goumans, M.-J., van Zonneveld, A. J., & ten Dijke, P. (2008) · Trends in Cardiovascular Medicine · doi:10.1016/j.tcm.2009.01.001 · Cited by 159
Atrioventricular cushion transformation is mediated by ALK2 in the developing mouse heart
Wang, J., Sridurongrit, S., Dudas, M., Thomas, P., Nagy, A., Schneider, M. D., Epstein, J. A., & Kaartinen, V. (2005) · Developmental Biology · doi:10.1016/j.ydbio.2005.07.035 · Cited by 158
Mercado-Pimentel, M. E., & Runyan, R. B. (2007) · Cells Tissues Organs · doi:10.1159/000101315 · Cited by 156
Generation of a mouse with conditionally activated signaling through the BMP receptor, ALK2
Fukuda, T., Scott, G., Komatsu, Y., Araya, R., Kawano, M., Ray, M. K., Yamada, M., & Mishina, Y. M. (2006) · genesis · doi:10.1002/dvg.20201 · Cited by 130
Hematopoietic Stem-Cell Contribution to Ectopic Skeletogenesis
Kaplan, F. S., Glaser, D. L., Shore, E. M., Pignolo, R. J., Xu, M., Zhang, Y., Emerson, S. G., Senitzer, D., & Forman, S. J. (2007) · Journal of Bone and Joint Surgery · doi:10.2106/00004623-200702000-00017 · Cited by 129
Self-citationAzhar, M., Runyan, R. B., Gard, C., Sanford, L. P., Miller, M. L., Andringa, A., Pawlowski, S. A., Rajan, S., & Doetschman, T. (2009) · Developmental Dynamics · doi:10.1002/dvdy.21854 · Cited by 128
Shore, E. M., & Kaplan, F. S. (2008) · Bone · doi:10.1016/j.bone.2008.05.013 · Cited by 122
Self-citationKizu, A., Medici, D., & Kalluri, R. (2009) · American Journal Of Pathology · doi:10.2353/ajpath.2009.090698 · Cited by 103
Self-citationLee, J. G., & Kay, E. P. (2006) · Experimental Eye Research · doi:10.1016/j.exer.2006.04.007 · Cited by 89
Skeletal metamorphosis in fibrodysplasia ossificans progressiva (FOP)
Kaplan, F. S., Shen, Q., Lounev, V. Y., et al. (2008) · Journal of Bone and Mineral Metabolism · doi:10.1007/s00774-008-0879-8 · Cited by 82
Self-citationActivin receptor-like kinase 2 can mediate atrioventricular cushion transformation.
Lai, Y.-T., Beason, K., Brames, G. P., Desgrosellier, J. S., Clegett, M. C., Shaw, M. V., Brown, C. B., & Barnett, J. (2000) · Developmental Biology · doi:10.1006/dbio.2000.9698 · Cited by 64
Okagawa, H., Markwald, R. R., & Sugi, Y. (2007) · Developmental Biology · doi:10.1016/j.ydbio.2007.03.015 · Cited by 35
Cited by
Molecular mechanisms of epithelial–mesenchymal transition
Lamouille, S., Xu, J., & Derynck, R. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3758 · Cited by 8,091
Signaling mechanisms of the epithelial-mesenchymal transition
Gonzalez, D. M., & Medici, D. (2014) · Science Signaling · doi:10.1126/scisignal.2005189 · Cited by 1,721
Self-citationWu, M., Chen, G., & Li, Y.-P. (2016) · Bone Research · doi:10.1038/boneres.2016.9 · Cited by 1,553
FGF/FGFR signaling in health and disease
Xie, Y., Su, N., Yang, J., Tan, Q., Huang, S., Jin, M., Ni, Z., Zhang, B., Zhang, D., Luo, F., et al. (2020) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-020-00222-7 · Cited by 904
The Ins and Outs of the Epithelial to Mesenchymal Transition in Health and Disease
Nieto, M. A. (2011) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-092910-154036 · Cited by 720
Metadata
- Authors
- Medici, D., Shore, E. M., Lounev, V. Y., Kaplan, F. S., Kalluri, R., & Olsen, B. R.
- Venue
- Nature Medicine
- Published
- 2010-11-21
- Volume
- 16
- Issue
- 12
- Pages
- 1400-1406
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W4248259718
- Cited by
- 678
- References
- 35
- FWCI
- 37.54
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
