Cited by 179Virus-based gene therapy researchCancer Research and TreatmentsTelomeres, Telomerase, and Senescence

In vivo imaging of lymph node metastasis with telomerase-specific replication-selective adenovirus

Kishimoto, Hiroyuki, Kojima, Toru, Watanabe, Yuichi, Kagawa, Shunsuke, Fujiwara, Toshiya, Uno, Futoshi, Teraishi, Fuminori, Kyo, Satoru +5 more·2006·Nature Medicine

Abstract

Currently available methods for detection of tumors in vivo such as computed tomography and magnetic resonance imaging are not specific for tumors. Here we describe a new approach for visualizing tumors whose fluorescence can be detected using telomerase-specific replication-competent adenovirus expressing green fluorescent protein (GFP) (OBP-401). OBP-401 contains the replication cassette, in which the human telomerase reverse transcriptase (hTERT) promoter drives expression of E1 genes, and the GFP gene for monitoring viral replication. When OBP-401 was intratumorally injected into HT29 tumors orthotopically implanted into the rectum in BALB/c nu/nu mice, para-aortic lymph node metastasis could be visualized at laparotomy under a three-chip color cooled charged-coupled device camera. Our results indicate that OBP-401 causes viral spread into the regional lymphatic area and selectively replicates in neoplastic lesions, resulting in GFP expression in metastatic lymph nodes. This technology is adaptable to detect lymph node metastasis in vivo as a preclinical model of surgical navigation.

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Crossref · 10.1038/nm1404

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Authors
Kishimoto, H., Kojima, T., Watanabe, Y., Kagawa, S., Fujiwara, T., Uno, F., Teraishi, F., Kyo, S., Mizuguchi, H., Hashimoto, Y., Urata, Y., Tanaka, N., & Fujiwara, T.
Affiliations
  • Okayama University (JP)
  • Kanazawa University (JP)
  • National Institute of Biomedical Innovation (JP)
  • Oncolys BioPharma (Japan) (JP)
Corresponding author
Fujiwara, Toshiyoshi
Venue
Nature Medicine
Published
2006-10
Volume
12
Issue
10
Pages
1213-1219
ISSN
  • 1078-8956 (ISSN-L)
  • 1546-170X
OpenAlex
W2144584725
Cited by
179
References
29
Citations by year
2026: 3 · 2025: 3 · 2024: 2 · 2023: 6 · 2022: 4 · 2021: 14 · 2020: 8 · 2019: 4 · 2018: 4 · 2017: 7 · 2016: 6 · 2015: 12 · 2014: 7 · 2013: 15 · 2012: 17
FWCI
6.26
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Medicine (publishedVersion)
  • PubMed (publishedVersion)
  • Okayama University Scientific Achievement Repository (Okayama University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
Keywords
lymph node metastasis, in vivo tumor imaging, telomerase, human telomerase reverse transcriptase, replication-competent adenovirus, green fluorescent protein, intratumoral injection, colorectal cancer, surgical navigation, nude mice
MeSH terms
Adenoviridae, Animals, Aorta, Disease Models, Animal, Female, Humans, Lymphatic Metastasis, Mice, Inbred BALB C, Mice, Nude, Neoplasm Metastasis, Neoplasm Transplantation, Microscopy, Video, Telomerase, Green Fluorescent Proteins, Mice

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