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High-efficiency labeling of sialylated glycoproteins on living cells
Open accessAbstract
We describe a simple method for efficiently labeling cell-surface sialic acid–containing glycans on living animal cells. The method uses mild periodate oxidation to generate an aldehyde on sialic acids, followed by aniline-catalyzed oxime ligation with a suitable tag. Aniline catalysis dramatically accelerates oxime ligation, allowing use of low concentrations of aminooxy-biotin at neutral pH to label the majority of cell-surface sialylated glycoproteins while maintaining high cell viability.
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Cited by
Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality
Sletten, E. M., & Bertozzi, C. R. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200900942 · Cited by 3,165
BODIPY-based probes for the fluorescence imaging of biomolecules in living cells
Kowada, T., Maeda, H., & Kikuchi, K. (2015) · Chemical Society Reviews · doi:10.1039/c5cs00030k · Cited by 1,381
Oximes and Hydrazones in Bioconjugation: Mechanism and Catalysis
Kölmel, D. K., & Kool, E. T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00090 · Cited by 699
Advances in the Biology and Chemistry of Sialic Acids
Chen, X., & Varki, A. (2010) · ACS Chemical Biology · doi:10.1021/cb900266r · Cited by 543
Algar, W. R., Prasuhn, D. E., Stewart, M. H., Jennings, T. L., Blanco-Canosa, J. B., Dawson, P. E., & Medintz, I. L. (2011) · Bioconjugate Chemistry · doi:10.1021/bc200065z · Cited by 502
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Metadata
- Authors
- Zeng, Y., Ramya, T. N. C., Dirksen, A., Dawson, P. E., & Paulson, J. C.
- Venue
- Nature Methods
- Published
- 2009-02-22
- Volume
- 6
- Issue
- 3
- Pages
- 207-209
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2065829276
- Cited by
- 430
- References
- 0
- FWCI
- 10.44
- Type
- journal article
- Language
- en
- Open access
- green
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- Zeng, Y., Ramya, T. N. C., Dirksen, A., Dawson, P. E., & Paulson, J. C. (2009). High-efficiency labeling of sialylated glycoproteins on living cells. Nature Methods. https://doi.org/10.1038/nmeth.1305
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