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High-efficiency labeling of sialylated glycoproteins on living cells
Open accessAbstract
We describe a simple method for efficiently labeling cell-surface sialic acid–containing glycans on living animal cells. The method uses mild periodate oxidation to generate an aldehyde on sialic acids, followed by aniline-catalyzed oxime ligation with a suitable tag. Aniline catalysis dramatically accelerates oxime ligation, allowing use of low concentrations of aminooxy-biotin at neutral pH to label the majority of cell-surface sialylated glycoproteins while maintaining high cell viability.
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Metadata
- Authors
- Zeng, Y., Ramya, T. N. C., Dirksen, A., Dawson, P. E., & Paulson, J. C.
- Venue
- Nature Methods
- Published
- 2009-02-22
- Volume
- 6
- Issue
- 3
- Pages
- 207-209
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2065829276
- Cited by
- 430
- References
- 0
- FWCI
- 10.44
- Type
- journal article
- Language
- en
- Open access
- green
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