The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
Open accessAbstract
Induced pluripotent stem cells (iPSCs) have been generated from somatic cells by transgenic expression of Oct4 (Pou5f1), Sox2, Klf4 and Myc. A major difficulty in the application of this technology for regenerative medicine, however, is the delivery of reprogramming factors. Whereas retroviral transduction increases the risk of tumorigenicity, transient expression methods have considerably lower reprogramming efficiencies. Here we describe an efficient piggyBac transposon–based approach to generate integration-free iPSCs. Transposons carrying 2A peptide–linked reprogramming factors induced reprogramming of mouse embryonic fibroblasts with equivalent efficiencies to retroviral transduction. We removed transposons from these primary iPSCs by re-expressing transposase. Transgene-free iPSCs could be identified by negative selection. piggyBac excised without a footprint, leaving the iPSC genome without any genetic alteration. iPSCs fulfilled all criteria of pluripotency, such as pluripotency gene expression, teratoma formation and contribution to chimeras. piggyBac transposon–based reprogramming may be used to generate therapeutically applicable iPSCs.
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Takahashi, K., & Yamanaka, S. (2006) · Cell · doi:10.1016/j.cell.2006.07.024 · Cited by 26,673
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse
Stadtfeld, M., Maherali, N., Breault, D. T., & Hochedlinger, K. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.02.001 · Cited by 823
Cited by
Koike-Yusa, H., Li, Y., Tan, E.-P., Velasco-Herrera, M. D. C., & Yusa, K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2800 · Cited by 1,064
Self-citationA hyperactive piggyBac transposase for mammalian applications
Yusa, K., Zhou, L., Li, M. A., Bradley, A., & Craig, N. L. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1008322108 · Cited by 770
Self-citationTargeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
Yusa, K., Rashid, S. T., Strick-Marchand, H., Varela, I., Liu, P.-Q., Paschon, D. E., Miranda, E., Ordóñez, A., Hannan, N. R. F., Rouhani, F. J., et al. (2011) · Nature · doi:10.1038/nature10424 · Cited by 639
Self-citationDNA Transposons: Nature and Applications in Genomics
Munoz-Lopez, M., & Garcia-Perez, J. (2010) · Current Genomics · doi:10.2174/138920210790886871 · Cited by 447
Somers, A., Jean, J.-C., Sommer, C. A., Omari, A., Ford, C. C., Mills, J. A., Ying, L., Sommer, A. G., Jean, J. M., Smith, B. W., et al. (2010) · Stem Cells · doi:10.1002/stem.495 · Cited by 423
Metadata
- Authors
- Yusa, K., Rad, R., Takeda, J., & Bradley, A.
- Venue
- Nature Methods
- Published
- 2009-03-31
- Volume
- 6
- Issue
- 5
- Pages
- 363-369
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2021581968
- Cited by
- 620
- References
- 3
- FWCI
- 23.40
- Type
- journal article
- Language
- en
- Open access
- green
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