The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
A hyperactive piggyBac transposase for mammalian applications
Open accessAbstract
DNA transposons have been widely used for transgenesis and insertional mutagenesis in various organisms. Among the transposons active in mammalian cells, the moth-derived transposon piggyBac is most promising with its highly efficient transposition, large cargo capacity, and precise repair of the donor site. Here we report the generation of a hyperactive piggyBac transposase. The active transposition of piggyBac in multiple organisms allowed us to screen a transposase mutant library in yeast for hyperactive mutants and then to test candidates in mouse ES cells. We isolated 18 hyperactive mutants in yeast, among which five were also hyperactive in mammalian cells. By combining all mutations, a total of 7 aa substitutions, into a single reading frame, we generated a unique hyperactive piggyBac transposase with 17-fold and ninefold increases in excision and integration, respectively. We showed its applicability by demonstrating an increased efficiency of generation of transgene-free mouse induced pluripotent stem cells. We also analyzed whether this hyperactive piggyBac transposase affects the genomic integrity of the host cells. The frequency of footprints left by the hyperactive piggyBac transposase was as low as WT transposase (~1%) and we found no evidence that the expression of the transposase affects genomic integrity. This hyperactive piggyBac transposase expands the utility of the piggyBac transposon for applications in mammalian genetics and gene therapy.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Bonner, W. M., Redon, C. E., Dickey, J. S., Nakamura, A. J., Sedelnikova, O. A., Solier, S., & Pommier, Y. (2008) · Nature reviews. Cancer · doi:10.1038/nrc2523 · Cited by 1,623
Ivics, Z., Hackett, P. B., Plasterk, R. H., & Izsvák, Z. (1997) · Cell · doi:10.1016/s0092-8674(00)80436-5 · Cited by 1,430
Cytological detection of mutagen–carcinogen exposure by sister chromatid exchange
Perry, P., & Evans, H. J. (1975) · Nature · doi:10.1038/258121a0 · Cited by 1,308
Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
Ding, S., Wu, X., Li, G., Han, M., Zhuang, Y., & Xu, T. (2005) · Cell · doi:10.1016/j.cell.2005.07.013 · Cited by 1,009
Mumberg, D., Muller, R., & Funk, M. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.25.5767 · Cited by 955
Mátés, L., Chuah, M. K. L., Belay, E., Jerchow, B., Manoj, N., Acosta-Sanchez, A., Grzela, D. P., Schmitt, A., Becker, K., Matrai, J., et al. (2009) · Nature Genetics · doi:10.1038/ng.343 · Cited by 948
P-element-mediated enhancer detection: a versatile method to study development in Drosophila.
Bellen, H. J., O'Kane, C. J., Wilson, C., Grossniklaus, U., Pearson, R. K., & Gehring, W. J. (1989) · Genes & Development · doi:10.1101/gad.3.9.1288 · Cited by 674
Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
Yusa, K., Rad, R., Takeda, J., & Bradley, A. (2009) · Nature Methods · doi:10.1038/nmeth.1323 · Cited by 620
Self-citationMammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system
Dupuy, A. J., Akagi, K., Largaespada, D. A., Copeland, N. G., & Jenkins, N. A. (2005) · Nature · doi:10.1038/nature03691 · Cited by 482
Spradling, A. C., Stern, D. M., Kiss, I., Roote, J., Laverty, T., & Rubin, G. M. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.24.10824 · Cited by 468
Cited by
Kosicki, M., Tomberg, K., & Bradley, A. (2018) · Nature Biotechnology · doi:10.1038/nbt.4192 · Cited by 1,883
Self-citationSun, F., Zeng, J., Jing, M., Zhou, J., Feng, J., Owen, S. F., Luo, Y., Li, F., Wang, H., Yamaguchi, T., et al. (2018) · Cell · doi:10.1016/j.cell.2018.06.042 · Cited by 837
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells
Yusa, K., Rashid, S. T., Strick-Marchand, H., Varela, I., Liu, P.-Q., Paschon, D. E., Miranda, E., Ordóñez, A., Hannan, N. R. F., Rouhani, F. J., et al. (2011) · Nature · doi:10.1038/nature10424 · Cited by 639
Self-citationA genetically encoded fluorescent sensor for rapid and specific in vivo detection of norepinephrine
Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A., Wu, Z., Wu, H., et al. (2019) · Neuron · doi:10.1016/j.neuron.2019.02.037 · Cited by 545
Next-generation GRAB sensors for monitoring dopaminergic activity in vivo
Sun, F., Zhou, J., Dai, B., Qian, T., Zeng, J., Li, X., Zhuo, Y., Zhang, Y., Wang, Y., Qian, C., et al. (2020) · Nature Methods · doi:10.1038/s41592-020-00981-9 · Cited by 490
Metadata
- Authors
- Yusa, K., Zhou, L., Li, M. A., Bradley, A., & Craig, N. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-01-04
- Volume
- 108
- Issue
- 4
- Pages
- 1531-1536
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2158771952
- Cited by
- 770
- References
- 40
- FWCI
- 10.93
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A hyperactive piggyBac transposase for mammalian applications. https://app.cochat.ai/papers/10.1073/pnas.1008322108
- The paper
- Yusa, K., Zhou, L., Li, M. A., Bradley, A., & Craig, N. L. (2011). A hyperactive piggyBac transposase for mammalian applications. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1008322108
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
