The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Fast, single-molecule localization that achieves theoretically minimum uncertainty
Open accessAbstract
We describe an iterative algorithm that converges to the maximum likelihood estimate of the position and intensity of a single fluorophore. Our technique efficiently computes and achieves the Cramér-Rao lower bound, an essential tool for parameter estimation. An implementation of the algorithm on graphics processing unit hardware achieved more than 105 combined fits and Cramér-Rao lower bound calculations per second, enabling real-time data analysis for super-resolution imaging and other applications.
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Citations
Cites
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Cited by
Super-resolution microscopy with DNA-PAINT
Schnitzbauer, J., Strauss, M. T., Schlichthaerle, T., Schueder, F., & Jungmann, R. (2017) · Nature Protocols · doi:10.1038/nprot.2017.024 · Cited by 1,047
Galectin-3 Coordinates a Cellular System for Lysosomal Repair and Removal
Jia, J., Claude-Taupin, A., Gu, Y., Choi, S. W., Peters, R., Bissa, B., Mudd, M. H., Allers, L., Pallikkuth, S., Lidke, K. A., et al. (2020) · Developmental Cell · doi:10.1016/j.devcel.2019.10.025 · Cited by 434
Self-citationQuantitative evaluation of software packages for single-molecule localization microscopy
Sage, D., Kirshner, H., Pengo, T., Stuurman, N., Min, J., Manley, S., & Unser, M. (2015) · Nature Methods · doi:10.1038/nmeth.3442 · Cited by 396
Subburaj, Y., Cosentino, K., Axmann, M., Pedrueza-Villalmanzo, E., Hermann, E., Bleicken, S., Spatz, J., & García-Sáez, A. J. (2015) · Nature Communications · doi:10.1038/ncomms9042 · Cited by 181
Single-molecule spectroscopy and imaging over the decades
Moerner, W. E., Shechtman, Y., & Wang, Q. (2015) · Faraday Discussions · doi:10.1039/c5fd00149h · Cited by 103
Metadata
- Authors
- Smith, C. S., Joseph, N., Rieger, B., & Lidke, K. A.
- Venue
- Nature Methods
- Published
- 2010-04-04
- Volume
- 7
- Issue
- 5
- Pages
- 373-375
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2075030758
- Cited by
- 522
- References
- 1
- FWCI
- 20.75
- Type
- journal article
- Language
- en
- Open access
- green
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- Smith, C. S., Joseph, N., Rieger, B., & Lidke, K. A. (2010). Fast, single-molecule localization that achieves theoretically minimum uncertainty. Nature Methods. https://doi.org/10.1038/nmeth.1449
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