The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Fast, high-contrast imaging of animal development with scanned light sheet–based structured-illumination microscopy
Open accessAbstract
Recording light-microscopy images of large, nontransparent specimens, such as developing multicellular organisms, is complicated by decreased contrast resulting from light scattering. Early zebrafish development can be captured by standard light-sheet microscopy, but new imaging strategies are required to obtain high-quality data of late development or of less transparent organisms. We combined digital scanned laser light-sheet fluorescence microscopy with incoherent structured-illumination microscopy (DSLM-SI) and created structured-illumination patterns with continuously adjustable frequencies. Our method discriminates the specimen-related scattered background from signal fluorescence, thereby removing out-of-focus light and optimizing the contrast of in-focus structures. DSLM-SI provides rapid control of the illumination pattern, exceptional imaging quality and high imaging speeds. We performed long-term imaging of zebrafish development for 58 h and fast multiple-view imaging of early Drosophila melanogaster development. We reconstructed cell positions over time from the Drosophila DSLM-SI data and created a fly digital embryo.
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Bayesian-Based Iterative Method of Image Restoration*
Richardson, W. H. (1972) · Journal of the Optical Society of America · doi:10.1364/josa.62.000055 · Cited by 4,160
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Chung, K., & Deisseroth, K. (2013) · Nature Methods · doi:10.1038/nmeth.2481 · Cited by 747
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Olarte, O. E., Andilla, J., Gualda, E. J., & Loza-Alvarez, P. (2018) · Advances in Optics and Photonics · doi:10.1364/aop.10.000111 · Cited by 298
Metadata
- Authors
- Keller, P. J., Schmidt, A. D., Santella, A., Khairy, K., Bao, Z., Wittbrodt, J., & Stelzer, E. H. K.
- Venue
- Nature Methods
- Published
- 2010-07-04
- Volume
- 7
- Issue
- 8
- Pages
- 637-642
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2050721997
- Cited by
- 528
- References
- 2
- FWCI
- 23.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Keller, P. J., Schmidt, A. D., Santella, A., Khairy, K., Bao, Z., Wittbrodt, J., & Stelzer, E. H. K. (2010). Fast, high-contrast imaging of animal development with scanned light sheet–based structured-illumination microscopy. Nature Methods. https://doi.org/10.1038/nmeth.1476
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