The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Protein localization in electron micrographs using fluorescence nanoscopy
Open accessAbstract
A complete portrait of a cell requires a detailed description of its molecular topography: proteins must be linked to particular organelles. Immunocytochemical electron microscopy can reveal locations of proteins with nanometer resolution but is limited by the quality of fixation, the paucity of antibodies and the inaccessibility of antigens. Here we describe correlative fluorescence electron microscopy for the nanoscopic localization of proteins in electron micrographs. We tagged proteins with the fluorescent proteins Citrine or tdEos and expressed them in Caenorhabditis elegans, fixed the worms and embedded them in plastic. We imaged the tagged proteins from ultrathin sections using stimulated emission depletion (STED) microscopy or photoactivated localization microscopy (PALM). Fluorescence correlated with organelles imaged in electron micrographs from the same sections. We used these methods to localize histones, a mitochondrial protein and a presynaptic dense projection protein in electron micrographs.
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Cites
Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Self-citationPrecise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Micheva, K. D., & Smith, S. J. (2007) · Neuron · doi:10.1016/j.neuron.2007.06.014 · Cited by 787
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Stimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
Visualizing presynaptic function
Kavalali, E. T., & Jorgensen, E. M. (2014) · Nature Neuroscience · doi:10.1038/nn.3578 · Cited by 126
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Wojcik, M., Hauser, M., Li, W., Moon, S., & Xu, K. (2015) · Nature Communications · doi:10.1038/ncomms8384 · Cited by 133
Two Rab2 Interactors Regulate Dense-Core Vesicle Maturation
Ailion, M., Hannemann, M., Dalton, S., Pappas, A., Watanabe, S., Hegermann, J., Liu, Q., Han, H.-F., Gu, M., Goulding, M. Q., et al. (2014) · Neuron · doi:10.1016/j.neuron.2014.02.017 · Cited by 86
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Metadata
- Authors
- Watanabe, S., Punge, A., Hollopeter, G., Willig, K. I., Hobson, R. J., Davis, M. W., Hell, S. W., & Jorgensen, E. M.
- Venue
- Nature Methods
- Published
- 2010-11-21
- Volume
- 8
- Issue
- 1
- Pages
- 80-84
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2118983672
- Cited by
- 351
- References
- 3
- FWCI
- 16.75
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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