The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Optogenetic manipulation of neural activity in freely moving Caenorhabditis elegans
Open accessAbstract
We present an optogenetic illumination system capable of real-time light delivery with high spatial resolution to specified targets in freely moving Caenorhabditis elegans. A tracking microscope records the motion of an unrestrained worm expressing channelrhodopsin-2 or halorhodopsin in specific cell types. Image processing software analyzes the worm's position in each video frame, rapidly estimates the locations of targeted cells and instructs a digital micromirror device to illuminate targeted cells with laser light of the appropriate wavelengths to stimulate or inhibit activity. Because each cell in an unrestrained worm is a rapidly moving target, our system operates at high speed (∼50 frames per second) to provide high spatial resolution (∼30 μm). To test the accuracy, flexibility and utility of our system, we performed optogenetic analyses of the worm motor circuit, egg-laying circuit and mechanosensory circuits that have not been possible with previous methods.
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Cites
The neural circuit for touch sensitivity in Caenorhabditis elegans
Chalfie, M., Sulston, J., White, J., Southgate, E., Thomson, J., & Brenner, S. (1985) · The Journal of Neuroscience · doi:10.1523/jneurosci.05-04-00956.1985 · Cited by 1,093
The structure of the ventral nerve cord of Caenorhabditis elegans
White, J. G., Southgate, E., Thomson, J. N., & Brenner, S. (1976) · Philosophical Transactions of the Royal Society of London. B, Biological Sciences · doi:10.1098/rstb.1976.0086 · Cited by 616
Optogenetic dissection of a behavioural module in the vertebrate spinal cord
Wyart, C., Bene, F. D., Warp, E., Scott, E. K., Trauner, D., Baier, H., & Isacoff, E. Y. (2009) · Nature · doi:10.1038/nature08323 · Cited by 409
Karbowski, J., Schindelman, G., Cronin, C. J., Seah, A., & Sternberg, P. W. (2008) · Journal of Computational Neuroscience · doi:10.1007/s10827-007-0054-6 · Cited by 87
Haspel, G., O'Donovan, M. J., & Hart, A. C. (2010) · The Journal of Neuroscience · doi:10.1523/jneurosci.2244-10.2010 · Cited by 71
Cited by
Optogenetics in neural systems
Yizhar, O., Fenno, L. E., Davidson, T. J., Mogri, M., & Deisseroth, K. (2011) · Neuron · doi:10.1016/j.neuron.2011.06.004 · Cited by 1,881
The development and application of optogenetics
Fenno, L., Yizhar, O., & Deisseroth, K. (2011) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-061010-113817 · Cited by 1,822
Two-photon optogenetic toolbox for fast inhibition, excitation and bistable modulation
Prakash, R., Yizhar, O., Grewe, B., Ramakrishnan, C., Wang, N., Goshen, I., Packer, A. M., Peterka, D. S., Yuste, R., Schnitzer, M. J., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2215 · Cited by 334
Proprioceptive Coupling within Motor Neurons Drives C. elegans Forward Locomotion
Wen, Q., Po, M. D., Hulme, E., Chen, S., Liu, X., Kwok, S. W., Gershow, M., Leifer, A. M., Butler, V., Fang-Yen, C., et al. (2012) · Neuron · doi:10.1016/j.neuron.2012.08.039 · Cited by 268
Self-citationPan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish
Kim, D. H., Kim, J., Marques, J. C., Grama, A., Hildebrand, D. G. C., Gu, W., Li, J. M., & Robson, D. N. (2017) · Nature Methods · doi:10.1038/nmeth.4429 · Cited by 228
Metadata
- Authors
- Leifer, A. M., Fang-Yen, C., Gershow, M., Alkema, M. J., & Samuel, A. D. T.
- Venue
- Nature Methods
- Published
- 2011-01-16
- Volume
- 8
- Issue
- 2
- Pages
- 147-152
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2112357421
- Cited by
- 319
- References
- 5
- FWCI
- 12.86
- Type
- journal article
- Language
- en
- Open access
- green
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